Modification of aflatoxin B<sub>1</sub>and ochratoxin A toxicokinetics in rats administered a yeast cell wall preparation

Firmin, Stéphane; Gandia, Peggy; Morgavi, Diego P.; Houin, G; Jouany, J. P.; Bertin, G.; Boudra, Hamid

doi:10.1080/19440041003801174

Cited by 30 publications

(17 citation statements)

References 37 publications

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“…Considering the half‐life (T 1/2 ) of AFB 1 which is about 64 h (Firmin et al . ) and to avoid any loss of AFB 1 , blood sample of rats in all groups was taken on days 18, 19 and 20. Rats were anesthetized using diethyl ether, and blood sample was taken by cardiac puncture from the artery vessel.…”

Section: Methodsmentioning

confidence: 99%

Reduction of aflatoxin level in aflatoxin-induced rats by the activity of probioticLactobacillus caseistrain Shirota

et al. 2013

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Aims: Aflatoxin B 1 (AFB 1 ) is considered as the most toxic food contaminant, and microorganisms, especially bacteria, have been studied for their potential to reduce the bioavailability of mycotoxins including aflatoxins. Therefore, this research investigated the efficacy of oral administration of Lactobacillus casei Shirota (LcS) in aflatoxin-induced rats. Methods and Results: Sprague Dawley rats were divided into three groups of untreated control, the group induced with AFB 1 only, and the group given probiotic in addition to AFB 1 . In the group induced with AFB 1 only, food intake and body weight were reduced significantly. The liver and kidney enzymes were significantly enhanced in both groups induced with AFB 1 , but they were lower in the group given LcS. AFB 1 was detected from all serum samples except for untreated control group's samples. Blood serum level of AFB 1 in the group induced with AFB 1 only was significantly higher than the group which received probiotic as a treatment (P < 0Á05), and there was no significant difference between the control group and the group treated with probiotic. Conclusions: LcS supplementation could improve the adverse effect of AFB 1 induction on rats' body weight, plasma biochemical parameters and also could reduce the level of AFB 1 in blood serum. Significance and Impact of the Study: This study's outcomes contribute to better understanding of the potential of probiotic to reduce the bioavailability ofAFB 1 . Moreover, it can open an opportunity for future investigations to study the efficacy of oral supplementation of probiotic LcS in reducing aflatoxin level in human.

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Section: Methodsmentioning

confidence: 99%

Reduction of aflatoxin level in aflatoxin-induced rats by the activity of probioticLactobacillus caseistrain Shirota

et al. 2013

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“…Organic adsorbents such as yeast cell wall (YCW) both in vitro and in vivo have shown much larger sorption capabilities across a wider spectrum of mycotoxins (Binder 2007;Huwig et al 2001;Jouany 2007;Yiannikouris et al 2004Yiannikouris et al , 2006Yiannikouris et al , 2013Karaman et al 2005;Kabak et al 2006;Kabak and Dobson 2009;Shetty and Jespersen 2006;Shetty et al 2007;Ringot et al 2007;Sabater-Vilar et al 2007;Korosteleva et al 2007;Matur et al 2010;Firmin et al 2010Firmin et al , 2011Joannis-Cassan et al 2011). Saccharomyces cerevisiae occurs as part of natural microbial population in food fermentation and as starter cultures in the food and beverage industries.…”

Section: Introductionmentioning

confidence: 99%

Development of an in vitro method for the prediction of mycotoxin binding on yeast-based products: case of aflatoxin B1, zearalenone and ochratoxin A

Faucet-Marquis

Joannis-Cassan

Hadjeba-Medjdoub

et al. 2014

Appl Microbiol Biotechnol

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To date, no official method is available to accurately define the binding capacity of binders. The goal is to define general in vitro parameters (equilibrium time, pH, mycotoxin/binder ratio) for the determination of binding efficacy, which can be used to calculate the relevant equilibrium adsorption constants. For this purpose, aflatoxin B1 (AFB1), zearalenone (ZEA) or ochratoxin A (OTA) were incubated with one yeast cell wall in pH 3, pH 5 or pH 7 buffers. The percentage of adsorption was recorded by quantitation of remaining mycotoxins in the supernatant and amount of mycotoxin adsorbed on the residue. The incubation of yeast cell wall in the presence of mycotoxins solved in buffer, lead to unexpected high adsorption percentage when the analysis was based only on remaining mycotoxins in the supernatant. The decrease of mycotoxins in the supernatant was not correlated to the amount of mycotoxins found in the residue. For this reason we modified the conditions of incubation. Yeast cell wall (5 mg) was pre-incubated in buffer (990 μl) at 37 °C during 5 min and then 10 μl of an alcoholic solution of mycotoxin (concentration 100 times higher than the final concentration required in the test tube) were added. After incubation, the solution was centrifuged, and the amount of mycotoxins were analysed both in the supernatant and in the residue. A plateau of binding was reached after 15 min of incubation whatever the mycotoxins and the concentrations tested. The adsorption of ZEA was better at pH 5 (75 %), versus 60 % at pH 3 and 7. OTA was only significantly adsorbed at pH 3 (50 %). Depending on the pH, the adsorptions of OTA or ZEA were increased or decreased when they were together, indicative of a cooperative effect.

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“…The inclusion of adsorbent materials in animal feed is perhaps the most economically viable means of achieving this "final-stage-control" of low-level or unaccounted for contamination [20]. Such supplementation can lead to the sequestration or adsorption of mycotoxins, thereby limiting the concentrations available to the animal until being excreted back into the environment [21,22] and/or promoting overall gut health, offsetting some of the related deleterious toxic effects [23].…”

Section: Introductionmentioning

confidence: 99%

Efficient Aflatoxin B1 Sequestration by Yeast Cell Wall Extract and Hydrated Sodium Calcium Aluminosilicate Evaluated Using a Multimodal In-Vitro and Ex-Vivo Methodology

Yiannikouris

Apajalahti

Kettunen

et al. 2021

Toxins

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In this work, adsorption of the carcinogenic mycotoxin aflatoxin B1 (AFB1) by two sequestrants—a yeast cell wall-based adsorbent (YCW) and a hydrated sodium calcium aluminosilicate (HSCAS)—was studied across four laboratory models: (1) an in vitro model from a reference method was employed to quantify the sorption capabilities of both sequestrants under buffer conditions at two pH values using liquid chromatography with fluorescence detection (LC-FLD); (2) in a second in vitro model, the influence of the upper gastrointestinal environment on the mycotoxin sorption capacity of the same two sequestrants was studied using a chronic AFB1 level commonly encountered in the field (10 µg/L and in the presence of feed); (3) the third model used a novel ex vivo approach to measure the absorption of 3H-labelled AFB1 in the intestinal tissue and the ability of the sequestrants to offset this process; and (4) a second previously developed ex vivo model readapted to AFB1 was used to measure the transfer of 3H-labelled AFB1 through live intestinal tissue, and the influence of sequestrants on its bioavailability by means of an Ussing chamber system. Despite some sorption effects caused by the feed itself studied in the second model, both in vitro models established that the adsorption capacity of both YCW and HSCAS is promoted at a low acidic pH. Ex vivo Models 3 and 4 showed that the same tested material formed a protective barrier on the epithelial mucosa and that they significantly reduced the transfer of AFB1 through live intestinal tissue. The results indicate that, by reducing the transmembrane transfer rate and reducing over 60% of the concentration of free AFB1, both products are able to significantly limit the bioavailability of AFB1. Moreover, there were limited differences between YCW and HSCAS in their sorption capacities. The inclusion of YCW in the dietary ration could have a positive influence in reducing AFB1′s physiological bioavailability.

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Modification of aflatoxin B₁and ochratoxin A toxicokinetics in rats administered a yeast cell wall preparation

Cited by 30 publications

References 37 publications

Reduction of aflatoxin level in aflatoxin-induced rats by the activity of probioticLactobacillus caseistrain Shirota

Reduction of aflatoxin level in aflatoxin-induced rats by the activity of probioticLactobacillus caseistrain Shirota

Development of an in vitro method for the prediction of mycotoxin binding on yeast-based products: case of aflatoxin B1, zearalenone and ochratoxin A

Efficient Aflatoxin B1 Sequestration by Yeast Cell Wall Extract and Hydrated Sodium Calcium Aluminosilicate Evaluated Using a Multimodal In-Vitro and Ex-Vivo Methodology

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Modification of aflatoxin B1and ochratoxin A toxicokinetics in rats administered a yeast cell wall preparation

Cited by 30 publications

References 37 publications

Reduction of aflatoxin level in aflatoxin-induced rats by the activity of probioticLactobacillus caseistrain Shirota

Reduction of aflatoxin level in aflatoxin-induced rats by the activity of probioticLactobacillus caseistrain Shirota

Development of an in vitro method for the prediction of mycotoxin binding on yeast-based products: case of aflatoxin B1, zearalenone and ochratoxin A

Efficient Aflatoxin B1 Sequestration by Yeast Cell Wall Extract and Hydrated Sodium Calcium Aluminosilicate Evaluated Using a Multimodal In-Vitro and Ex-Vivo Methodology

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Modification of aflatoxin B₁and ochratoxin A toxicokinetics in rats administered a yeast cell wall preparation