Modification of a SLA titanium surface with calcium-containing nanosheets and its effects on osteoblast behavior

Abstract

The aim of this study was to present a procedure to prepare a calcium-containing nanosheets-modified sandblasted and acid etched (SLA) titanium surface and explore its effects on osteoblast behavior.

Biocorrosion of pure and SLA titanium surfaces in the presence of Porphyromonas gingivalis and its effects on osteoblast behavior

“…36 Changes in the characteristics of titanium surfaces can directly inuence cell adhesion behavior. 37,38 In this study, the decreased adherent MC3T3-E1 cells and poor cell spreading on the Ti-pg and SLA-pg surfaces were observed. This result conrmed that pure and SLA titanium surfaces pre-corroded by P. gingivalis negatively affected the adhesion and spreading abilities of osteoblasts.…”

“…Runx2 is a vital transcription factor for osteoblast differentiation which expressed in the early stage of osteogenic differentiation. 38 As a mediating marker for osteogenic differentiation, OPN is mainly related to osteoblast maturation in the relatively early stage. 39 The western blotting data clearly showed declined expressions of Runx2 and OPN on the two titanium surfaces.…”

“…[43][44][45] In our previous study, it was also conrmed that nanosheets modied titanium surface, which revealed superhydrophilicity, could dramatically enhance the cell adhesion and spreading. 38 Furthermore, preferential growth and matrix mineralization of osteoblasts have been observed on hydrophilic substrates. 40 As the rst stage of interactions between cell and biomaterial, initial cell adhesion plays a key role in regulating the subsequent cell growth and differentiation.…”

Applications of Nanosheets in Frontier Cellular Research

“…Functionalized nanosheets were also synthesized onto traditional medical materials for improving cell culture. For example, when calcium-containing nanosheets were synthesized onto a sandblasted and acid-etched (SLA) titanium surface, a sustained release of Ca 2+ ions was measured, and adhesion/spreading of MC3T3-E1 cells was upregulated [ 34 ]. Coating of flower-like calcium phosphate nanosheets onto a titanium surface can also promote cell adhesion and spreading due to its wettability [ 35 ].…”

The Effects of Combined Micron-Scale Surface and Different Nanoscale Features on Cell Response

“…erefore, many researchers try to obtain surface implants with micropits by sandblasting-acidetching (SLA) and then use the methods of anodic oxidation [16], double-etching [17], or synthesized nanosheet [18] to prepare nanopores on micro pits, forming a mixed morphology of micron and nano to further enhance their biological activity.…”