Applications of Nanosheets in Frontier Cellular Research

Huang, Wenjing; Sunami, Yuta; Kimura, Hiroshi; Zhang, Sheng

doi:10.3390/nano8070519

Cited by 27 publications

(18 citation statements)

References 124 publications

(175 reference statements)

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“…The induced alteration of cells’ proliferative potential was demanded as one of the most possible modes of many 2D materials’ action. − According to our best knowledge, there is still a lack of fundamental information on the influence of MXenes on cellular cycle and DNA synthesis. To face this issue, we carried out studies on cellular death type, which also confirmed the results of MTT assay (time- and dose-dependent cytotoxicity of examined nanostructures).…”

Section: Results and Discussionmentioning

confidence: 99%

Juggling Surface Charges of 2D Niobium Carbide MXenes for a Reactive Oxygen Species Scavenging and Effective Targeting of the Malignant Melanoma Cell Cycle into Programmed Cell Death

Jastrzębska

Szuplewska

Rozmysłowska-Wojciechowska

et al. 2020

ACS Sustainable Chem. Eng.

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There are several problems that need to be overcome to enable smooth and sustainable clinical translation of the MXene materials, including hard controllable surface chemistry. In this study, we show for the first time that, by using surface modification with poly-l-lysine (PLL), it is possible to completely invert the highly negative surface charge of the 2D niobium carbide MXenes (viz., Nb2C and Nb4C3) toward a highly positive value. Switching the surface charge of MXenes results in obtaining important biological effects in vitro such as targeting of malignant cells and inducing cell cycle arrest at the G0/G1 phase and triggering apoptosis, i.e., programmed cell deaththe most desirable effect for designing anticancer nanodrugs, which should directly target the cells’ physiology instead of generating direct toxicity. In addition, both 2D Nb2C/PLL and Nb4C3/PLL MXenes showed significant adjustment of their biocompatibilities in relation to normal skin cells. The obtained results suggest that Nb-MXenes can be used as scavengers for the reactive oxygen species (ROS). This study formulates an important step toward further development of MXene-based nanotherapies and strategies for cancer cell elimination in relation to standard therapeutic procedures currently being developed for these materials.

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Section: Results and Discussionmentioning

confidence: 99%

Juggling Surface Charges of 2D Niobium Carbide MXenes for a Reactive Oxygen Species Scavenging and Effective Targeting of the Malignant Melanoma Cell Cycle into Programmed Cell Death

Jastrzębska

Szuplewska

Rozmysłowska-Wojciechowska

et al. 2020

ACS Sustainable Chem. Eng.

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“…The enhanced cytotoxic and apoptotic activity of the biogenic sulfur can be attributed to the many advantages of the 2D nanosheet morphology such as the large specific surface area for cell adhesion and internalization, abundance of sulfur atoms in ultrathin nanosheets, high capability of release of sulfur atoms, high polarized charges, and convenient attachment of different functional groups. 30,31 A study by Loutfy et al on the cytotoxic effect of graphene oxide nanosheets toward HepG2 cancer cells has shown that the cytotoxic effect was due to the strong interaction with the phospholipid layer leading to internalization into mitochondria, nucleus, and cytoplasm, causing membrane integrity loss and damage. 62 Further, SNP-B exhibited higher cytotoxic effects against cancer cell lines while showing minimum toxicity to normal cells compared to SNP-C.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Studies have proved that cells can internalize nanosheets, resulting in a higher interaction between the substrate and cells . Internalization and cellular distribution will have a direct impact on the cytotoxicity of the nanosheets . In the present study, sulfur nanosheets have shown a higher cytotoxicity compared to sulfur nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

Krishnappa

Chandraprabha

et al. 2021

ACS Omega

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Two-dimensional nanostructures have gained tremendous interest in the field of biomedical applications and cancer activity in particular. Although sulfur is known for its wide range of biological activities, its potentiality in two-dimensional forms as an antitumor agent is hitherto unexplored. To address the current deficient knowledge on nano-sulfur as an antitumor agent, we report the synthesis of nano-sulfur sheets/particles and their cytotoxic, apoptotic activity against human carcinoma cell lines. In vitro cytotoxic effects of biogenic nanosheets (SNP-B) and chemogenic nanoparticles (SNP-C) were assessed against human lung carcinoma (A549), human epidermoid carcinoma (A431), human promyelocytic leukaemia (HL60) and human lung fibroblast (IMR90) cell lines. Cell cycle analysis, apoptotic study, and caspase-3 expression studies were carried out to understand the mechanism of cytotoxic activity of nano-sulfur. The MTT assay indicated a dose-dependent decrease in viability of all the cell lines treated with nano-sulfur, with SNP-B being more toxic compared to SNP-C. The apoptotic study and cell cycle analysis indicated cell cycle arrest followed by apoptosis-induced cell death. The caspase-3 expression study indicated that nano-sulfur induces apoptosis by the activation of caspase through the mitochondrial pathway. Apart from this, a lower cytotoxicity was observed in IMR90 cell lines treated with SNP-B , indicating a higher specificity of synthesized nanosheets towards cancer cells. Taken all together, this work highlights the potentiality of sulfur nanosheets in inducing cytotoxicity and apoptotic activity, and the impact of morphology as a critical determinant on the cytotoxic response on various cell lines.

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“…In the last decade, nanosheets have been incorporated into a broad range of biosensor applications. [283] The high SA/V ratio allows for increased interaction between the sensor surface and analytes of interest. [284] Additionally, the 2D confinement of electrons in these materials can yield unique optical and electrical properties.…”

Section: Nanosheetsmentioning

confidence: 99%

Advances in Biosensors and Diagnostic Technologies Using Nanostructures and Nanomaterials

Welch

Powell

Clevinger

et al. 2021

Adv Funct Materials

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Nanoscale materials have unique properties that make them especially useful for biomedical diagnostic applications. Recent developments in nanoengineering have resulted in increasing use of nanostructures in biosensors. Various types of 0D, 1D, 2D, and 3D nanostructures have been used to improve biosensor sensitivity, selectivity, limit of detection, and time to result, among other metrics. These nanostructures have been integrated into electrochemical, optical, and other biosensors for this purpose. Here, the most recent advances in the use of nanostructured materials in biosensors are described. This includes a discussion of nanoparticles, nanorods, nanofibers, nanopillars, nanowires, nanosheets, indented nanopatterns (nanoholes and nanoslits), nanogaps, nanochannels, nanopores, nanofunctionalized surfaces, and complex hierarchical structures and their unique advantages and applications in biosensors. Clinical applications of these nanobiosensors are also highlighted along with a discussion of the future directions for these materials in diagnostics.

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Applications of Nanosheets in Frontier Cellular Research

Cited by 27 publications

References 124 publications

Juggling Surface Charges of 2D Niobium Carbide MXenes for a Reactive Oxygen Species Scavenging and Effective Targeting of the Malignant Melanoma Cell Cycle into Programmed Cell Death

Juggling Surface Charges of 2D Niobium Carbide MXenes for a Reactive Oxygen Species Scavenging and Effective Targeting of the Malignant Melanoma Cell Cycle into Programmed Cell Death

Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

Advances in Biosensors and Diagnostic Technologies Using Nanostructures and Nanomaterials

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