Modification de métabolisme des ARN induites par l'adrénaline dans les cellules animales

Decallonne, Jacques R.; Briquet, Michel; Lambert, Richard; Wiaux, A. L.

doi:10.1007/bf02144519

Experientia

1967

DOI: 10.1007/bf02144519

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Modification de métabolisme des ARN induites par l'adrénaline dans les cellules animales

Jacques R. Decallonne

Michel Briquet

Richard Lambert

et al.

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1968

1977

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Cited by 6 publications

(4 citation statements)

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confidence: 95%

“…However, it has already been reported (Decallonne, Briquet & Wiaux, 1967) that adrenaline induced some cytoplasmic modifications in rat liver cells with respect to the turnover of both mRNA 16S and rRNA 29S and 18S.We tried to establish differences between the normal incorporation rate of orotic acid into DNA and nuclear RNA and the rate of incorporation into liver tissue treated with [14C]adrenaline in order to find the hormonal site of action.Rat liver slices, weighing about 500 mg., were incubated with [14C]orotic acid hydrate (sp.act. 3\m=.\2 mc/mm).…”

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confidence: 96%

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Incorporation of [14c]adrenaline Into the Nuclei of Rat Liver Cells

Decallonne¹,

Leblus²,

Wiaux³

1968

Journal of Endocrinology

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A direct relationship between a hormone and a template like DNA or nuclear RNA in rat liver nuclei has, so far, not been established. However, it has already been reported (Decallonne, Briquet & Wiaux, 1967) that adrenaline induced some cytoplasmic modifications in rat liver cells with respect to the turnover of both mRNA 16S and rRNA 29S and 18S.We tried to establish differences between the normal incorporation rate of orotic acid into DNA and nuclear RNA and the rate of incorporation into liver tissue treated with [14C]adrenaline in order to find the hormonal site of action.Rat liver slices, weighing about 500 mg., were incubated with [14C]orotic acid hydrate (sp.act. 3\m=.\2 mc/mm). Each portion of liver was suspended in 10 ml. Ringer solution (5. 10\ m=-\ 3 m-tris-HCl buffer of pH 7\m=.\4 at 38\ m=. \ 2\ s=deg\ , kept under O2:CO2 (95 % :5 %) and 0\m=.\1 \g=m\c[14C]orotic acid/100 mg. liver was added at intervals of 10 min. for 10-90 min. The nuclei were obtained from the cells by the method of Roodyn (1962).Each 500 mg. portion of liver was first washed with 5.10-3 M-tris-HCl buffer at pH 7-4 and then homogenized in 6 ml. of a mixture of saccharose : CaCl2 (0-25 M : 0-0018 m) cooled to 4°. The homogenate was then suspended in 6 ml. of a saccharose : CaCl2 solution (0-34 m : 0-0018 m) and centrifugated for 10 min. at 700 g. The resulting pellet was re-centrifugated twice or three times under the same conditions. By this procedure an efficiency of about 50-70 % with respect to the nuclei and with very little contamination was obtained. The nuclei were then broken down with 9 ml. of a 5 % aqueous solution of sodium dodecyl sulphate (SDS)/100 ml. Microscopic examination showed that this treatment for 3 min. at 37°affected practically all nuclei. This was also controlled by u.v. spectrophotometry at 265 rap. The amount of DNA extracted was used as a basis for the incorporation calculations. The result of the DNA deter¬ minations (300 ±15 pg. DNA/100mg. liver) agrees with that of Paoletti (1963). The incorporation of [14C]orotic acid was expressed as counts/min./100/¿g. DNA. An increase from 50 + 10 counts/min. at 10 min. to 5850 ± 10 counts/min. at 90 min. was found with an average background of about 10 counts/min. When adrenaline (0-5 10~3 mg./100 mg.) was added to the incubation medium, together with orotic acid, there was an inhibition of about 95 % in orotic acid incorporation into the nuclei. This pronounced inhibition took place as soon as the hormone was added to the incubate. Since adrenaline inhibits the incorporation of this common precursor of DNA and RNA into the nucleus, it seems likely that adrenaline itself does not enter the cell nuclei but remains in the cytoplasmic fraction where the inhibition takes place.

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mentioning

confidence: 95%

mentioning

confidence: 96%

Incorporation of [14c]adrenaline Into the Nuclei of Rat Liver Cells

Decallonne¹,

Leblus²,

Wiaux³

1968

Journal of Endocrinology

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“…While the role of catecholamines in carbohydrate metabolism is well known (Sutherland & Robinson, 1966) we have little knowledge of the relationship between these hormones and the metabolism of proteins, in particular the biosynthesis of nucleic acids (Decallonne, Briquet, Lambert & Wiaux, 1967) Since noradrenaline (NA) appears to exert some regulatory control on cell function (Iversen, 1967), we have studied the effect of this chemical mediator on the metabolism of RNA and polyamines (spermine and spermidine). Previous work has demonstrated the existence of a close relationship between these components; in particular, aliphatic polyamines were shown to enhance the synthesis of both nuclear and cytoplasmic nucleic acids in chick embryos when the content of spermine and spermidine was experimentally increased (Moruzzi, Barbiroli & Caldarera, 1968 To demonstrate the effect of NA on the synthesis of polyamines and RNA we used chick embryos on the 8th day of incubation; the hormone was injected into the air space.…”

mentioning

confidence: 99%

The Effect of Noradrenaline on Polyamine and Rna Synthesis in the Chick Embryo

Caldarera¹,

Giorgi²,

Casti³

1970

Journal of Endocrinology

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While the role of catecholamines in carbohydrate metabolism is well known (Sutherland & Robinson, 1966) we have little knowledge of the relationship between these hormones and the metabolism of proteins, in particular the biosynthesis of nucleic acids (Decallonne, Briquet, Lambert & Wiaux, 1967) Since noradrenaline (NA) appears to exert some regulatory control on cell function (Iversen, 1967), we have studied the effect of this chemical mediator on the metabolism of RNA and polyamines (spermine and spermidine). Previous work has demonstrated the existence of a close relationship between these components; in particular, aliphatic polyamines were shown to enhance the synthesis of both nuclear and cytoplasmic nucleic acids in chick embryos when the content of spermine and spermidine was experimentally increased (Moruzzi, Barbiroli & Caldarera, 1968). 15 30 45 60 Minutes after hormone treatment Spermidine Spermine 15 30 45 60 Minutes after hormone treatmentFig. 2 Fig. 1Fig. 1. Effect of noradrenaline on incorporation of [3H]formate into RNA of chick embryos. Each point is the average of three experiments. The vertical lines indicate the s.E. -, Control; A-A-treatment.Fig. 2. Effect of noradrenaline on incorporation of [14C]putrescine into spermine and spermidine of chick embryos. Each point is the average of three experiments. The vertical lines indicate the s.E. -#, Control; A-A. treatment.To demonstrate the effect of NA on the synthesis of polyamines and RNA we used chick embryos on the 8th day of incubation; the hormone was injected into the air space.[3H]Formate (25 \g=m\c) was injected 5 hr. before hormone treatment as an RNA

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“…This in turn should reflect changes at the level of nucleic acid metabolism. Evidence exists for a stimulatory action by catecholamines on precursor incorporation into proteins (Mallov, 1973(Mallov, , 1976Kallfelt et al, 1976), RNA and polyamines (Decalonne et al, 1967;Caldarera et al, 1970Caldarera et al, , 1971.…”

mentioning

confidence: 99%

Modifications of major aspects of myocardial ribonucleic acid metabolism as a response to noradrenaline. Behaviour of polyadenylate polymerase and ribonucleic acid polymerase, acetylation of histones and rate of synthesis of polyamines

Casti¹,

Corti²,

Reali³

et al. 1977

Biochemical Journal

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Noradrenaline added to perfused rabbit heart previously perfused with labelled precursors causes, after 2.5 and 5.0 min, a general increase of specific radioactivity or RNA in subcellular fractions, but no augmentation of acetylation of F2a2 and F2a1 histone fractions and no stimulation of DNA-dependent RNA polymerase activities. Synthesis of spermidine and spermine is enhanced at 10.0 min of treatment, when there is also a fall in specific radioactivity of RNA. The cytoplasmic Mn2+-stimulated polyadenylate polymerase activity is strongly enhanced 30s to 2.5 min after injection of noradrenaline or of dibutyryl cyclic AMP. Both the cyclic nucleotide and noradrenaline have no influence in vitro on the polyadenylate polymerase reaction.

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Modification de métabolisme des ARN induites par l'adrénaline dans les cellules animales

Cited by 6 publications

References 5 publications

Incorporation of [14c]adrenaline Into the Nuclei of Rat Liver Cells

Incorporation of [14c]adrenaline Into the Nuclei of Rat Liver Cells

The Effect of Noradrenaline on Polyamine and Rna Synthesis in the Chick Embryo

Modifications of major aspects of myocardial ribonucleic acid metabolism as a response to noradrenaline. Behaviour of polyadenylate polymerase and ribonucleic acid polymerase, acetylation of histones and rate of synthesis of polyamines

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