Modern on-site tool for monitoring contamination of halal meat with products from five non-halal animals using multiplex polymerase chain reaction coupled with DNA strip

Denyingyhot, Anat; Srinulgray, Theerarak; Mahamad, Pornpimol; Ruangprach, Arnakorn; Sa-I, Suwainah; Saerae, Tuanyasmin; Vesaratchavest, Mongkol; Dahlan, Winai; Keeratipibul, Suwimon

doi:10.1016/j.foodcont.2021.108540

Cited by 21 publications

(17 citation statements)

References 50 publications

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“…The labeled amplicons bind to the antibodies fixed on the DNA strips, which allow red staining indicating the presence or absence of a target. Products were visible within 15 min, and 100% detection accuracy was found for beef meatball samples containing 10% and 1% of nonhalal targets (Denyingyhot et al., 2022). With the LFI method, multiplexing or viewing results simultaneously on a single strip can be challenging; however, Denyingyhot et al.…”

Section: Dna‐based Methods For Game Species Identificationmentioning

confidence: 99%

“…The DNA strips function similarly to the LFI strips. Denyingyhot et al (2022) developed this approach as a rapid on-site technique for detecting five nonhalal meat species (monkeys, dogs, rats, pigs, and cats) in halalcertified foods. In this method, the products of biotinlabeled primers are hybridized to a probe.…”

Section: 34mentioning

confidence: 99%

“…The labeled amplicons bind to the antibodies fixed on the DNA strips, which allow red staining indicating the presence or absence of a target. Products were visible within 15 min, and 100% detection accuracy was found for beef meatball samples containing 10% and 1% of nonhalal targets (Denyingyhot et al, 2022). With the LFI method, multiplexing or viewing results simultaneously on a single strip can be challenging; however, Denyingyhot et al (2022) were able to depict the outcome of an mPCR on a single DNA strip by varying the tagged primers on their strip.…”

Section: 34mentioning

confidence: 99%

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A systematic review of DNA‐based methods in authentication of game and less common meat species

Adenuga

Montowska

2023

Comp Rev Food Sci Food Safe

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Despite the numerous studies on food safety and authenticity, especially for meat and meat products, not enough studies have been conducted focusing exclusively on game species and other unusual meat animals. As a result of the European horse scandal, the horse is currently the target of many meat authenticity studies. With this review, we aim to present various DNA-based methods that have been used by researchers to identify, detect, and quantify game, uncommon meat animals, and wildlife species. Polymerase chain reaction (PCR) is considered the standard method for DNA analysis in meat authenticity testing. However, in this paper, we present several other methods that may or may not involve the PCR technique. For this purpose, we systematically reviewed 131 articles selected according to various criteria such as target animal species, method of analysis, year of publication, and so forth. The result of our study shows the most studied game and uncommon meat species, PCR-and non-PCR-based methods for game meat analysis, and DNA-based methods in wildlife conservation. With this study, researchers can find detailed information about frequent game species used as adulterants for regular meat products and the DNA-based techniques to identify them.

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Section: Dna‐based Methods For Game Species Identificationmentioning

confidence: 99%

Section: 34mentioning

confidence: 99%

“…The labeled amplicons bind to the antibodies fixed on the DNA strips, which allow red staining indicating the presence or absence of a target. Products were visible within 15 min, and 100% detection accuracy was found for beef meatball samples containing 10% and 1% of nonhalal targets (Denyingyhot et al, 2022). With the LFI method, multiplexing or viewing results simultaneously on a single strip can be challenging; however, Denyingyhot et al (2022) were able to depict the outcome of an mPCR on a single DNA strip by varying the tagged primers on their strip.…”

Section: 34mentioning

confidence: 99%

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A systematic review of DNA‐based methods in authentication of game and less common meat species

Adenuga

Montowska

2023

Comp Rev Food Sci Food Safe

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“…In addition, the regulation of halal status is indeed important to assure the quality and standard of halal meat products [ 28 ]. This can be accomplished through an official certification and placement of an official logo on the products as a mean to provide security to consumers [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ].…”

Section: Halal Meat Productsmentioning

confidence: 99%

The Application of Chemometrics in Metabolomic and Lipidomic Analysis Data Presentation for Halal Authentication of Meat Products

et al. 2022

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The halal status of meat products is an important factor being considered by many parties, especially Muslims. Analytical methods that have good specificity for the authentication of halal meat products are important as quality assurance to consumers. Metabolomic and lipidomic are two useful strategies in distinguishing halal and non-halal meat. Metabolomic and lipidomic analysis produce a large amount of data, thus chemometrics are needed to interpret and simplify the analytical data to ease understanding. This review explored the published literature indexed in PubMed, Scopus, and Google Scholar on the application of chemometrics as a tool in handling the large amount of data generated from metabolomic and lipidomic studies specifically in the halal authentication of meat products. The type of chemometric methods used is described and the efficiency of time in distinguishing the halal and non-halal meat products using chemometrics methods such as PCA, HCA, PLS-DA, and OPLS-DA is discussed.

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“…Adulteration through the addition of non-halal ingredients is a major concern for Muslims. Every Muslim is forbidden from using non-halal ingredients or eating non-halal food (Denyingyhot et al, 2022;Halimi et al, 2021). Meanwhile, it is not easy to distinguish non-halal mixed meat from beef, so developing a reliable detection technique for mixing ingredients needs is necessary (Prachugsorn et al, 2022;Saputra et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Design and Performance Test of Specific Primers to Detect Bovine DNA Fragments using Multiplex PCR Technique for Halal Authentification

Kusnadi

Hernandi

Al-Awwaly

et al. 2022

Indonesian Journal of Halal Research

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Adulterating meat products with several species, including non-halal species, is often found in commercial products. Therefore, non-halal ingredients are a major source of concern for Muslims. Multiplex polymerase chain reaction (PCR) with multiple primers can detect contamination of meat components from non-halal species in a single reaction process, making it more effective and efficient. Multiplex PCR is a PCR technique that uses multiple primers and DNA samples in one reaction to amplify multiple target regions. In this study, a pair of species-specific primers encoding the Cytochrome c oxidase subunit I (CO1) gene were designed to amplify bovine DNA, tested for specificity, and applied in multiplex PCR technique together with D-loop primers for pigs, Cyt-b for rats, and 12S rRNA for dogs. The CO1 primers, along with D-loop primers for porcine, Cyt-b primers for rats, and 12S rRNA primers for dogs, can be used to detect specific bovine DNA with a size of 279 bp and sequence similarity of 96%.

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Modern on-site tool for monitoring contamination of halal meat with products from five non-halal animals using multiplex polymerase chain reaction coupled with DNA strip

Cited by 21 publications

References 50 publications

A systematic review of DNA‐based methods in authentication of game and less common meat species

A systematic review of DNA‐based methods in authentication of game and less common meat species

The Application of Chemometrics in Metabolomic and Lipidomic Analysis Data Presentation for Halal Authentication of Meat Products

Design and Performance Test of Specific Primers to Detect Bovine DNA Fragments using Multiplex PCR Technique for Halal Authentification

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