Modern Methods for Angiogenesis Assessment in Clinical Practice

Мавликеев, М. О.; Titova, Angelina; Gudz, D.O.; Деев, Р. В.

doi:10.23888/nmj20171110-123

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…However, fluorescent visualization of the vascular bed is not a label-free method. The fluorescein emits in the visible range, therefore, it is impossible to explore the deep-sited pathological foci, and the indocyanine green interacts with the blood proteins and has a low fluorescence yield [29][30][31][32].…”

Section: Methods Of Blood Flow Assessmentmentioning

confidence: 99%

Current Methods for the Assessment of Oxygen Status and Biotissue Microcirculation Condition: Diffuse Optical Spectroscopy (Review)

Бесчастнов¹,

Ryabkov²,

Pavlenko³

et al. 2018

Sovrem Tehnol Med

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The problem of studying the oxygen status and biotissue microcirculation is of special interest for many directions in medical science since one of the causes of hypoxia development as a typical pathophysiological process is a microcirculatory "failure" associated with the impairment of normal anatomy of the capillary wall, changes in the rheological blood properties, acceleration or slowdown of the blood flow. Current imaging techniques enable the researchers to study the processes of biosystem vital activity at various levels: from organs and tissues to the substance molecular composition. Methods of functional bioimaging implemented into clinical practice provide the opportunity of watching online the processes of substance movement in the body, monitoring blood flow parameters, assessing hypoxia level, characterizing metabolism in greater detail, and, at the same time, correcting timely pathological conditions. The main advantages and disadvantages of bioimaging examination methods such as BOLD functional magnetic resonance tomography, positron emission tomography, optical imaging, laser Doppler flowmetry, and transcutaneous oximetry are considered in the present review. Special attention is paid to diffuse optical spectroscopy as a noninvasive method of lifetime study of substance content in biotissues. The principle of diffuse optical spectroscopy is based on the ability of tissue chromophores (oxyhemoglobin, deoxyhemoglobin, fatty acids, collagen) to absorb diffusely scattered light of a definite wavelength. Their concentrations are calculated with the allowance for the absorption coefficients of chromophores. Diffuse optical spectroscopy is being introduced in clinical practice to diagnose the degree of tumor malignization, evaluate vascularization in reconstructive operations, diagnose hypoxic tissue conditions, monitor intraoperatively blood flow parameters, measure hypoxia levels in diabetes mellitus. It provides the possibility to define and make clear indications to skin plastic surgery and, conceivably, to develop new methods of skin plasty.

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Section: Methods Of Blood Flow Assessmentmentioning

confidence: 99%

Current Methods for the Assessment of Oxygen Status and Biotissue Microcirculation Condition: Diffuse Optical Spectroscopy (Review)

Бесчастнов¹,

Ryabkov²,

Pavlenko³

et al. 2018

Sovrem Tehnol Med

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“…An effective way to close such chronic wounds is free skin plastic surgery using a split flap [5]. It is known that successful engrafting of an autologous transplant requires active angiogenesis [6][7][8]. Under the normal conditions, a skin graft placed in the recipient wound is nutrient-supplied by diffusion and the early signs of vascularization are observed on day 5 after surgery [9].…”

Section: Introductionmentioning

confidence: 99%

Hypoxic Preconditioning of the Skin Graft Donor Area: Clinical and Morphological Assessment

Orlinskaya¹,

Davydenko²,

Bagryantsev³

et al. 2018

Sovrem Tehnol Med

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The aim of the study was to evaluate the method of hypoxic preconditioning of the donor area using clinical and morphological assessment of the autologous dermal transplant (skin graft). Materials and Methods. The transplants were prepared from the skin donor area after hypoxic preconditioning. Reparative regeneration, skin graft angiogenesis and the cosmetic status of the donor area wound upon healing were studied. To this end, five patients with diabetes-associated soft tissue wounds underwent classic free skin plastic surgery (control group), and five patients underwent the similar operation preceded by hypoxic preconditioning of the donor zone (study group). On day 5 after the surgery, morphological and immunohistochemical examination of the skin transplant was performed; on day 14, the area of engraftment was evaluated. Results. On day 14, the area of engraftment of the autologous transplant in the control group was 56 [52; 64]%, and in the study group-91 [84; 95]% (p=0.007). The healing of the donor area wounds in the control group was detected on day 18 [16; 21], in the study group-on day 14 [12; 17] (p=0.002). In the intravital histological staining of the biopsy specimen, cell-enriched skin fragments were found in the study group, whereas only cell-depleted skin fragments were observed in the control group. Upon the double sequential immunohistochemical staining with antibodies to Ki-67 and CD31 antigens, increased proliferation of endothelial cells of the dermis was noted in the study group, with the proliferation index of 29 [22; 33] versus 17 [13; 24]% in the control group (p=0.011). The increased levels of antibodies to VEGF and Fli-1 in the study group also pointed to the active angiogenesis in these patients. Conclusion. The results of the intravital histological and immunohistochemical study indicate that hypoxic preconditioning of the donor area can serve an effective tool of increasing the reparative regeneration and angiogenesis in the skin graft.

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Diagnosis of Postoperative Pelvic Adhesions in Women With Chronic Pelvic Pain on the Background of Undifferentiated Connective Tissue Dysplasia

Репина¹,

Dmitrieva²

2018

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Кафедра акушерства и гинекологии, Рязанский государственный медицинский университет им. акад. и.п. павлова, Рязань, Российская Федерация 2 Кафедра математики, физики и медицинской информатики, Рязанский государственный медицинский университет им. акад. и.п. павлова, Рязань, Российская Федерация Цель: оптимизировать диагностическую точность послеоперационного спаечного процесса на фоне недифференцированной дисплазии соединительной ткани (нДСТ) у пациенток с хронической тазовой болью. Материал и методы: исследование проведено на базе ГБУ РО «Областной клинический перинатальный центр», в ходе которого проанализировано 60 пациенток с диагнозом трубно-перитонеальное бесплодие. Пациентки разделены на 2 группы на основании модифицированной балльной шкалы Т.И. Кадуриной. Проведены исследование генофенотипических предикторов путём анализа выраженности нДСТ методом профилирования фенотипических маркёров по модифицированной шкале Т.И. Кадуриной; изучение полиморфизма генов VEGF 634 [rs2010963] и IL6-174 [rs1800795] и оценка выраженности спаечного процесса согласно классификационной системе Американского общества фертильности, генетической предрасположенности к развитию спаечного процесса методом генотипирования ацетилирования и интенсивности боли по визуально-аналоговой шкале. Результаты: к группе высокого риска развития тазовых перитонеальных спаек (ТПС) отнесены пациентки с выраженностью баллов нДСТ, равной 10 и более по модифицированной шкале Т.И. Кадуриной. Диагностика предрасположенности к спаечному процессу методами определения типа ацетилирования и УЗИ позволяет предположить наличие спаек в малом тазу до операции у 76,66% и 65% обследованных соответственно. Молекулярно-генетическими предикторами нДСТ являются C/C IL6-174 аллель C и C/G VEGFA 634 аллель G. Интенсивность хронической тазовой боли при спаечном процессе зависит от стадии последнего. Для прогнозирования риска развития ТПС следует использовать разработанную математическую модель на основании P = 1/(1+еЂ(-z)) и независимых предикторов: миопия, пролапс митрального клапана, IL6-174 [rs1800795], VEGFA 634 [rs2010963] и фенотип ацетилирования. Заключение: применение метода бинарной логистической регрессии на основании клинико-анамнестических, инструментальных и молекулярно-генетических данных даёт возможность определить вероятность развития ТПС на фоне недифференцированной ДСТ по формуле: P = 1/(1+еЂ(-z)). Ключевые слова: тазовые перитонеальные спайки, хроническая тазовая боль, ацетилирование, бинарная логистическая регрессия. Для цитирования: Бен Салха М, Репина НБ, Дмитриева МН. Методы диагностики послеоперационного спаечного процесса в малом тазу у женщин с хронической тазовой болью на фоне недифференцированной дисплазии соединительной ткани.

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Modern Methods for Angiogenesis Assessment in Clinical Practice

Cited by 3 publications

References 4 publications

Current Methods for the Assessment of Oxygen Status and Biotissue Microcirculation Condition: Diffuse Optical Spectroscopy (Review)

Current Methods for the Assessment of Oxygen Status and Biotissue Microcirculation Condition: Diffuse Optical Spectroscopy (Review)

Hypoxic Preconditioning of the Skin Graft Donor Area: Clinical and Morphological Assessment

Diagnosis of Postoperative Pelvic Adhesions in Women With Chronic Pelvic Pain on the Background of Undifferentiated Connective Tissue Dysplasia

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