Moderate-intensity, premeal cycling blunts postprandial increases in monocyte cell surface CD18 and CD11a and endothelial microparticles following a high-fat meal in young adults

Abstract

<jats:p>High-fat meals promote transient increases in proatherogenic factors, implicating the postprandial state in cardiovascular disease (CVD) progression. Although low-grade inflammation is associated with CVD, little research has assessed postprandial inflammation. Because of its anti-inflammatory properties, premeal exercise may counteract postprandial inflammation. The purpose of this study was to determine postprandial alterations in monocytes and circulating markers of endothelial stress and inflammation following a high-fat meal in young adults with or without premeal cycle exercise. Each subject completed two trials and was randomized to rest or cycle at a moderate intensity prior to eating a high-fat meal. Flow cytometry was used to assess monocyte cell surface receptor expression and concentration of endothelial microparticles (EMP). Plasma cytokines were assessed using Luminex MagPix. Statistical analysis was completed using separate linear mixed models analyses with first-order autoregressive (AR(1)) heterogeneous covariance structure. Significance was set at P ≤ 0.05. Percentage increases in classic monocyte CD11a and CD18 were greater overall in the postprandial period in the meal-only condition compared with the meal + exercise condition (P &lt; 0.05). EMP concentration was 47% greater 3 h after the meal compared with premeal values in the meal-only condition (P &lt; 0.05); no significant increase was observed in the meal + exercise condition. Premeal cycling blunted postprandial increases in EMP and CD11a and CD18. Acute, moderate-intensity exercise may help counteract possibly deleterious postprandial monocyte and endothelial cell activation.</jats:p>

Dynamic microvesicle release and clearance within the cardiovascular system: triggers and mechanisms

“…Exercise before consumption of the high-fat meal also altered the release of MVs although with different effects between studies. Harrison et al [80] noted that the rise in endothelial MVs was not attenuated by exercise before the meal, despite a considerable reduction in postprandial lipaemia [80], whereas another study found that prior moderate intensity cycling can blunt the postprandial increases in endothelial MVs [84].…”

Endurance Capacity Is Not Correlated with Endothelial Function in Male University Students

“…It has been reported that exercise reduced the level of circulating EMPs as a new mechanism of protecting endothelial function. A single bout of prior endurance activity partially prevented the increase of circulating EMPs after a fat meal [16], [17], in parallel with the reduction of reactive oxygen species production in circulating CD31+ cells. But Sossdorf reported the increment of the circulating microparticles delayed to return to the baseline level after 2 hours of the exercise in sedentary individuals, compared with those trained controls, which might contribute to an exercise-induced increase of hemostatic potential [31].…”

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function <em>In Vitro</em>

“…Our area of research focuses on a study of how exercise and dietary habits influence immune system health and function 6,[8][9][10][18][19][20] . Such objectives have significant practical implications for a variety of areas of human health.…”

“…Over the past ten years, our laboratory, among others, has been keenly focused on the effect of various nutritional and exercise treatments patterns on innate immune function [6][7][8][9] . The method demonstrated in this manuscript has practical implications within the field of clinical immunology.…”