Models of Inflammation: Carrageenan Air Pouch

Duarte, Djane B.; Vasko, Michael R.; Fehrenbacher, Jill C.

doi:10.1002/0471141755.ph0506s72

Cited by 41 publications

(27 citation statements)

References 28 publications

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“…In our experimental conditions the intraplantar injection of 1% carrageenan solution developed a biphasic response: an early inflammatory reaction and neurogenic pain, lasting for 6 h, and a second late phase (inflammator y hyperalgesia), with a peak intensity at 72 h and which progressively diminished after approximately 96 h [33,34]. The positive control drug IND determined a significant reduction in local inflammator y process after intraperitoneal administration, its effects being concordant with the reported literature data regarding the effects of this non-steroidal anti-inflammator y drug in this experimental model in rodents [12,13,35,36].…”

Section: Resultssupporting

confidence: 83%

“…The degree of local inflammatory edema and its duration was assessed by using a plethysmograph (PanLab Apparatus). We measured the posterior paw volume according to the following scheme: before the induction of edema (initial volume at moment zero), at 24 h and 3 days after the inflammation was developed [13,14]. The results were expressed as percentage of reduction in inflammation, compared to initial volume in control animals.…”

Section: Experimental Part Materials and Methodsmentioning

confidence: 99%

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The Effects of Two Nitric Oxide Donors in Acute Inflammation in Rats Experimental data

Buca¹,

Mititelu-Tarțău²,

Rezuş³

et al. 2018

Rev. Chim.

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We aimed to investigate the effects of two nitric oxide donors in acute inflammation in rats. The experiment was carried out on white Wistar rats, randomly distributed in 4 groups of 5 animals each; the substances were administered intraperitoneally as follows: Group 1 (SS): saline solution 0.1mL/100 g body weight (control); Group 2 (IND): indometacin 150 mg/kg body weight; Group 3 (NEB): nebivolol 1 mg/kg body weight; Group 4 (GSNO): S-nitroso-glutathione 1 mg/kg body weight. An experimental model of acute hind paw inflammation with carrageenan was used for the researches. The influence of the nitric oxide donors on blood parameters, specific inflammatory and immune markers was evaluated 24 h, respectively 72 hours after the injection of irritant agent. The experimental protocol was implemented according to the recommendations of our University Committee for Research and Ethical Issues. The administration of nitric oxide donors nebivolol and S-nitroso-glutathione was accompanied by a substantial diminution of paw edema, as well as by an important decrease in the percent of lymphocytes, a reduction of interleukin 6 and tumor necrosis factor alpha values. The effects of nebivolol were more accentuated than of S-nitroso-glutathione, but less intense than of indomethacin in the experiment. The treatment with nebivolol and S-nitroso-glutathione produced anti-inflammatory effects on local acute inflammation in the carrageenan-induced paw edema test in rats.

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Section: Resultssupporting

confidence: 83%

Section: Experimental Part Materials and Methodsmentioning

confidence: 99%

The Effects of Two Nitric Oxide Donors in Acute Inflammation in Rats Experimental data

Buca¹,

Mititelu-Tarțău²,

Rezuş³

et al. 2018

Rev. Chim.

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“…Whether this is also the case in vivo remains to be elucidated. The observation that injected carrageenan causes inflammation [ 28 , 29 ] could even be explored as a potential adjuvant. Studies analyzing the attachment mechanisms of HPVs have shown that on epithelial cells, HPVs use heparan sulfate proteoglycans (HSPGs) as primary attachment factors [ 30 – 32 ].…”

Section: Discussionmentioning

confidence: 99%

Non-human papillomaviruses for gene delivery in vitro and in vivo

et al. 2018

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Papillomavirus capsids are known to have the ability to package DNA plasmids and deliver them both in vitro and in vivo. Of all known papillomavirus types, human papillomaviruses (HPVs) are by far the most intensely studied. Although HPVs work well as gene transfer vectors, their use is limited as most individuals are exposed to this virus either through a HPV vaccination or natural infection. To circumvent these constraints, we produced pseudovirions (PsVs) of ten non-human papillomavirus types and tested their transduction efficiencies in vitro. PsVs based on Macaca fascicularis papillomavirus-11 and Puma concolor papillomavirus-1 were further tested in vivo. Intramuscular transduction by PsVs led to months-long expression of a reporter plasmid, indicating that PsVs have potential as gene delivery vectors.

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“…These findings suggest that the complex effectively released constituents capable of interfering in the production of chemical mediators, such as vasoactive amines and prostaglandins, responsible for vasodilation and the formation of inflammatory exudates [ 76 ]. A recent study by Martins et al (2017) showed that the systemic administration of same C. rhamnifolioides essential oil at doses of 25, 50, 100, and 200 mg/kg resulted in a significant reduction of carrageenan and dextran-induced edema [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Physicochemical Characterization of the Croton rhamnifolioides Essential Oil Inclusion Complex in β-Cyclodextrin

Martins

Wanderley

Alcântara

et al. 2020

Biology

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Croton rhamnifolioides is used in popular medicine for the treatment of inflammatory diseases. The objective of this study was to characterize and evaluate the anti-inflammatory effect of C. rhamnifolioides essential oil complexed in β-cyclodextrin (COEFC). The physicochemical characterization of the complexes was performed using different physical methods. The anti-inflammatory activity was evaluated in vivo by ear edema, paw edema, cotton pellet-induced granuloma, and vascular permeability by Evans blue extravasation. The mechanism of action was validated by molecular docking of the major constituent into the cyclooxygenase-2 (COX-2 enzyme). All doses of the COEFC reduced acute paw edema induced by carrageenan and dextran, as well as vascular permeability. Our results suggest the lowest effective dose of all samples inhibited the response induced by histamine or arachidonic acid as well as the granuloma formation. The complexation process showed that the pharmacological effects were maintained, however, showing similar results using much lower doses. The results demonstrated an involvement of the inhibition of pathways dependent on eicosanoids and histamine. Complexation of β-cyclodextrin/Essential oil (β-CD/EO) may present an important tool in the study of new compounds for the development of anti-inflammatory drugs.

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Models of Inflammation: Carrageenan Air Pouch

Cited by 41 publications

References 28 publications

The Effects of Two Nitric Oxide Donors in Acute Inflammation in Rats Experimental data

The Effects of Two Nitric Oxide Donors in Acute Inflammation in Rats Experimental data

Non-human papillomaviruses for gene delivery in vitro and in vivo

Anti-Inflammatory and Physicochemical Characterization of the Croton rhamnifolioides Essential Oil Inclusion Complex in β-Cyclodextrin

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