The Effects of Two Nitric Oxide Donors in Acute Inflammation in Rats Experimental data

Abstract

We aimed to investigate the effects of two nitric oxide donors in acute inflammation in rats. The experiment was carried out on white Wistar rats, randomly distributed in 4 groups of 5 animals each; the substances were administered intraperitoneally as follows: Group 1 (SS): saline solution 0.1mL/100 g body weight (control); Group 2 (IND): indometacin 150 mg/kg body weight; Group 3 (NEB): nebivolol 1 mg/kg body weight; Group 4 (GSNO): S-nitroso-glutathione 1 mg/kg body weight. An experimental model of acute hind paw inflammation with carrageenan was used for the researches. The influence of the nitric oxide donors on blood parameters, specific inflammatory and immune markers was evaluated 24 h, respectively 72 hours after the injection of irritant agent. The experimental protocol was implemented according to the recommendations of our University Committee for Research and Ethical Issues. The administration of nitric oxide donors nebivolol and S-nitroso-glutathione was accompanied by a substantial diminution of paw edema, as well as by an important decrease in the percent of lymphocytes, a reduction of interleukin 6 and tumor necrosis factor alpha values. The effects of nebivolol were more accentuated than of S-nitroso-glutathione, but less intense than of indomethacin in the experiment. The treatment with nebivolol and S-nitroso-glutathione produced anti-inflammatory effects on local acute inflammation in the carrageenan-induced paw edema test in rats.

Bisphosphonates Influence and Pain Assessment in Mobilization of Patients with Fragility Fracture of the Pelvis

“…Some studies indicate the mechanism of reactive oxygen species as a possible cause of bone fragility [19][20][21][22], other consider homocysteine as a possible cause for the disease [23][24][25][26]. Many literature data show that homocysteine levels are directly linked to oxidative mechanims and both may be involved in bone remodeling [27,28,29,30].…”

The Influence of Nitric Oxid Donors Nebivolol and S-Nitrosoglutathion of the Oxidatives Stress and Liver Function in Rats

“…For the study, the animals were randomly allocated into 4 groups of 5 rats each, treated intraperitoneal, as follows: group 1 (Control) received saline solution 0.1 mL/100 g body weight; group 2 (IND) received indomethacin 150 mg/kg body weight (kbw); groups 3 (NEB) and 4 (GSNO) received nebivolol 1 mg/kbw, respectively S-nitrosoglutathione 1 mg/kbw. Indomethacin (IND) (a methylated indole derivative from the acetic acid group of the nonsteroidal anti-inflammatory drugs) was used as a positive control substance, with demonstrated anti-inflammatory activity in this experimental model of acute inflammation in rats [12,13].…”

The Role of Phytosterols in Nonalcoholic Fatty Liver Disease

“…Desmosterol (C 27 H 44 O) along with endothelial nitric oxide synthase is a major regulator of inflammation with a role in maintaining mitochondrial function in the liver. The decline in nitric oxide synthase activity has been shown to be correlated with the progression of NAFLD [ 37 , 38 ]. Elevated desmosterol levels have been associated with liver inflammation.…”