Models for the self-assembly of basement membrane.

Abstract

Basement membranes contain a number of intrinsic macromolecular components which are unique to these structures and which cooperatively assemble into specific heteropolymeric matrices. Type IV collagen triple helical monomers bind together at their amino-terminal, carboxy-terminal, and lateral domains to form a lattice-like array. Laminin, in a two-step process, binds to itself at its terminal globular domains to form polymers and also binds collagen at two I This work was supported by a Career Development Award from the Juvenile Diabetes Foundation (PDY), Hull Endowment Fund (ECT), NIH training grant GM-07562 (ASC), and NIH grant AM-30556 (HF). 2 Presented in part at the symposium on the BASEMENT MEMBRANE FUNCTION AND MORPHOGENESIS, May 5, 1985, as part of the program at the annual meeting of the Histochemical Society, held in Crystal City,

Heparanase and a Synthetic Peptide of Heparan Sulfate-interacting Protein Recognize Common Sites on Cell Surface and Extracellular Matrix Heparan Sulfate

“…HIP is a recently described cell surface HP/HS-binding protein (25,26) and contains a peptide motif that is capable of discriminating between secreted, cell surface, and ECM forms of HS. 2 Therefore, it was considered that heparanase and HIP peptide might recognize similar motifs in HS. As shown in Fig.…”

“…During the formation of metastases, migrating cells are confronted by natural tissue barriers, such as connective tissue stroma and basal lamina (2,3). The ability of malignant cells to penetrate these barriers depends upon the presence of enzymes capable of degrading extracellular matrix (ECM) 1 components (1)(2)(3)(4)(5). For these reasons, considerable effort has been focused on the study of tissue-degradative enzymes produced and secreted by metastatic tumor cells as well as normal cells of the tissue being invaded.…”

“…Furthermore, HIP also appears to bind the anticoagulantly active species of HP efficiently and with high affinity. 2 HP octasaccharides, but not hexasaccharides, are large enough to bind HIP with high affinity. Thus, HIP appears to recognize a motif that at least overlaps the anticoagulant motif in HP and HS chains.…”

“…We previously reported heparanase degradation of cell surface HS subpopulations of the human adenocarcinoma cell line RL95. In the present study, heparanase activity was examined on RL95 cell surface HS subpopulations in the presence of a synthetic peptide (CRPKAKAKAKAK-DQTK) of heparin/heparan sulfate-interacting protein (HIP; Liu, S., Smith, S. E., Julian, J., Rohde Metastasis occurs via a sequential and complex series of interactions between tumor cells and normal host cells and tissues (1,2). During the formation of metastases, migrating cells are confronted by natural tissue barriers, such as connective tissue stroma and basal lamina (2,3).…”

“…In the present study, heparanase activity was examined on RL95 cell surface HS subpopulations in the presence of a synthetic peptide (CRPKAKAKAKAK-DQTK) of heparin/heparan sulfate-interacting protein (HIP; Liu, S., Smith, S. E., Julian, J., Rohde Metastasis occurs via a sequential and complex series of interactions between tumor cells and normal host cells and tissues (1,2). During the formation of metastases, migrating cells are confronted by natural tissue barriers, such as connective tissue stroma and basal lamina (2,3). The ability of malignant cells to penetrate these barriers depends upon the presence of enzymes capable of degrading extracellular matrix (ECM) 1 components (1)(2)(3)(4)(5).…”