Modelling the effects of post-FESS middle turbinate synechiae on sinonasal physiology: A computational fluid dynamics study

Khatri, Hershil; Salati, Hana; Wong, Eugene; Bradshaw, Kimberley; Inthavong, Kiao; Sacks, Raymond; Singh, Narinder

doi:10.1016/j.anl.2023.04.003

Cited by 4 publications

(4 citation statements)

References 37 publications

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“…The use of 3D printing emerges as a rapid and efficient means to replicate nasal structures, facilitating experimental tests to evaluate nasal function. This underscores the significance of 3D printing as a valuable auxiliary tool for conducting nasal pressure measurements [ 38 , 39 ]. Computer methods for quantifying the sinus cavity contents on CT have shown potential, but existing methods rely on manual or semiautomatic segmentation of the sinus cavity, which is also time consuming and unsuitable for routine clinical use.…”

Section: Discussionmentioning

confidence: 99%

Preliminary study on AI-assisted diagnosis of bone remodeling in chronic maxillary sinusitis

Zou,

Ji,

Cui

et al. 2024

BMC Med Imaging

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Objective To construct the deep learning convolution neural network (CNN) model and machine learning support vector machine (SVM) model of bone remodeling of chronic maxillary sinusitis (CMS) based on CT image data to improve the accuracy of image diagnosis. Methods Maxillary sinus CT data of 1000 samples in 500 patients from January 2018 to December 2021 in our hospital was collected. The first part is the establishment and testing of chronic maxillary sinusitis detection model by 461 images. The second part is the establishment and testing of the detection model of chronic maxillary sinusitis with bone remodeling by 802 images. The sensitivity, specificity and accuracy and area under the curve (AUC) value of the test set were recorded, respectively. Results Preliminary application results of CT based AI in the diagnosis of chronic maxillary sinusitis and bone remodeling. The sensitivity, specificity and accuracy of the test set of 93 samples of CMS, were 0.9796, 0.8636 and 0.9247, respectively. Simultaneously, the value of AUC was 0.94. And the sensitivity, specificity and accuracy of the test set of 161 samples of CMS with bone remodeling were 0.7353, 0.9685 and 0.9193, respectively. Simultaneously, the value of AUC was 0.89. Conclusion It is feasible to use artificial intelligence research methods such as deep learning and machine learning to automatically identify CMS and bone remodeling in MSCT images of paranasal sinuses, which is helpful to standardize imaging diagnosis and meet the needs of clinical application.

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Section: Discussionmentioning

confidence: 99%

Preliminary study on AI-assisted diagnosis of bone remodeling in chronic maxillary sinusitis

Zou,

Ji,

Cui

et al. 2024

BMC Med Imaging

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“…However, the nasal cavity undergoes significant changes with the onset of CRS, including significant mucus production, mucosal swelling, possible polyp formation, and decreased epithelial barrier integrity ( 28 ). Consequently, nasal obstruction and decreased airflow are often seen in CRS patients, which can be alleviated through endoscopic sinus surgery ( 3 , 29 – 32 ). Additionally, inflammation and bacterial infection, key components of CRS pathology, have been shown to increase skin temperatures in post-surgery wounds and skin ulcers ( 33 , 34 ).…”

Section: Introductionmentioning

confidence: 99%

Temperature influences commensal-pathogen dynamics in a nasal epithelial cell co-culture model

Huffines,

Boone,

Kiedrowski

2024

mSphere

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Chronic rhinosinusitis (CRS) is an inflammatory disease of the paranasal sinuses, and microbial dysbiosis associated with CRS is thought to be a key driver of host inflammation that contributes to disease progression. Staphylococcus aureus is a common upper respiratory tract (URT) pathobiont associated with higher carriage rates in CRS populations, where S. aureus -secreted toxins can be identified in CRS tissues. Although many genera of bacteria colonize the URT, few account for the majority of sequencing reads. These include S. aureus and several species belonging to the genus Corynebacterium , including Corynebacterium propinquum and Corynebacterium pseudodiphtheriticum , which are observed at high relative abundance in the healthy URT. Studies have examined bacterial interactions between major microbionts of the URT and S. aureus , but few have done so in the context of a healthy versus diseased URT environment. Here, we examine the role of temperature in commensal, pathogen, and epithelial dynamics using an air-liquid interface cell culture model mimicking the nasal epithelial environment. Healthy URT temperatures change from the nares to the nasopharynx and are increased during disease. Temperatures representative of the healthy URT increase persistence and aggregate formation of commensal C. propinquum and C. pseudodiphtheriticum , reduce S. aureus growth, and lower epithelial cytotoxicity compared to higher temperatures correlating with the diseased CRS sinus. Dual-species colonization revealed species-specific interactions between Corynebacterium species and S. aureus dependent on temperature. Our findings suggest URT mucosal temperature plays a significant role in mediating polymicrobial and host-bacterial interactions that may exacerbate microbial dysbiosis in chronic URT diseases. IMPORTANCE Chronic rhinosinusitis is a complex inflammatory disease with a significant healthcare burden. Although presence of S. aureus and microbial dysbiosis are considered mediators of inflammation in CRS, no studies have examined the influence of temperature on S. aureus interactions with the nasal epithelium and the dominant genus of the healthy URT, Corynebacterium . Interactions between Corynebacterium species and S. aureus have been documented in several studies, but none to date have examined how environmental changes in the URT may alter their interactions with the epithelium or each other. This study utilizes a polarized epithelial cell culture model at air-liquid interface to study the colonization and spatial dynamics of S. aureus and clinical isolates of Corynebacterium from people with CRS to characterize the role temperature has in single- and dual-species dynamics on the nasal epithelium.

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“…However, the nasal cavity undergoes significant changes with the onset of CRS, including significant mucus production, mucosal swelling, possible polyp formation, and decreased epithelial barrier integrity (28). Consequently, nasal obstruction and decreased airflow are often seen in CRS patients, which can be alleviated through endoscopic sinus surgery (3,(29)(30)(31)(32). Additionally, inflammation and bacterial infection, key components of CRS pathology, have been shown to increase skin temperatures in postsurgery wounds and skin ulcers (33,34).…”

Section: Introductionmentioning

confidence: 99%

Temperature Influences Commensal-Pathogen Dynamics in a Nasal Epithelial Cell Co-culture Model

Huffines,

Boone,

Kiedrowski

2023

Preprint

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Chronic rhinosinusitis (CRS) is an inflammatory disease of the paranasal sinuses, and microbial dysbiosis associated with CRS is thought to be a key driver of host inflammation that contributes to disease progression.Staphylococcus aureusis a common upper respiratory tract (URT) pathobiont that is associated with higher carriage rates in CRS populations, whereS. aureussecreted toxins can be identified in CRS tissue samples. Although many genera of bacteria colonize the URT, relatively few account for the majority of sequencing reads. These includeS. aureus, as well as several species belonging to the genusCorynebacterium, includingCorynebacterium propinquumandCorynebacterium pseudodiphtheriticum, which are observed at high relative abundance in the URT of healthy individuals. Studies have examined the bacterial interactions between the major microbionts of the URT andS. aureus, but few have done so in the context of a healthy versus diseased URT environment. Here, we examine the role of temperature in commensal, pathogen, and epithelial dynamics using an air-liquid interface cell culture model mimicking the nasal epithelial environment. The healthy URT temperature changes from the nares to the nasopharynx and is altered during disease. Temperatures representative of the healthy URT increase persistence and aggregate formation of commensalC. propinquum and C. pseudodiphtheriticum, reduceS. aureusgrowth, and lower epithelial cytotoxicity compared to higher temperatures correlating with the diseased CRS sinus. Dual-species colonization revealed species-specific interactions between commensalCorynebacteriumspecies andS. aureusdependent on temperature. Our findings suggest that URT mucosal temperature plays a significant role in mediating polymicrobial and host-bacterial interactions that may exacerbate microbial dysbiosis found in chronic URT disease.IMPORTANCEChronic rhinosinusitis is a complex inflammatory disease with a significant healthcare burden. Although presence ofS. aureusand microbial dysbiosis are considered mediators of inflammation in CRS, no studies have examined the influence of temperature onS. aureusinteractions with the nasal epithelium and the dominant genus of the healthy URT,Corynebacterium. Interactions betweenCorynebacterium speciesandS. aureushave been documented in several studies, but none to date have examined how environmental changes in the URT may alter their interactions with the epithelium or each other. This study utilizes a polarized epithelial cell culture model at air-liquid interface to study the colonization and spatial dynamics ofS. aureusand clinical isolates ofCorynebacteriumfrom people with CRS to characterize the role temperature has in single-and dual-species dynamics on the nasal epithelium.

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Modelling the effects of post-FESS middle turbinate synechiae on sinonasal physiology: A computational fluid dynamics study

Cited by 4 publications

References 37 publications

Preliminary study on AI-assisted diagnosis of bone remodeling in chronic maxillary sinusitis

Preliminary study on AI-assisted diagnosis of bone remodeling in chronic maxillary sinusitis

Temperature influences commensal-pathogen dynamics in a nasal epithelial cell co-culture model

Temperature Influences Commensal-Pathogen Dynamics in a Nasal Epithelial Cell Co-culture Model

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