Modelling the biosensor utilising parallel substrates conversion

Ašeris, Vytautas; Baronas, Romas; Kulys, Juozas

doi:10.1016/j.jelechem.2012.06.025

Cited by 14 publications

(10 citation statements)

References 27 publications

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“…The calculations show that at the high concentrations of catalase and peroxidase the decrease of the membrane thickness of 19 % can decrease the half‐time by almost 53 %. The steady‐state response is less sensitive to membrane thickness, but the decrease of it will cause an almost proportional increase of the steady‐state response if the bioelectrode is acting under internal diffusion limitation 11.…”

Section: Resultsmentioning

confidence: 99%

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Electrochemical Peroxidase‐Catalase Clark‐Type Biosensor: Computed and Experimental Response

et al. 2013

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A bioelectrode containing immobilized catalase and peroxidase was built using a Clark-type oxygen electrode. The bioelectrode responded to hydrogen peroxide (H 2 O 2 ) as well as to acetaminophen (Ac). The sensitivity of the bioelectrode for H 2 O 2 was 0.35 mM O 2 /mM H 2 O 2 and for Ac it was 0.23-1.05 mM O 2 /mM Ac at pH 6.6 and 25 8C. The limit of detection of Ac varied from 12 to 44 mM. The half-time of the bioelectrode response to hydrogen peroxide was 36 s. The modeling of the bioelectrode action was performed digitally at transition and steady-state conditions using finite difference technique. The calculated half-time of the bioelectrode response to hydrogen peroxide was 53 % larger and the steady-state response 11 % less than experimentally determined. The response to Ac was 2-3 times smaller in comparison to the experimental values. The calculated response change correlated with the experimentally determined when the catalase and peroxidase concentrations in the biocatalytical membrane changed 3-4 orders of magnitude. The simulations of the bioelectrode response revealed that the bioelectrode acts in diffusion limiting conditions at almost all enzymes concentrations. The model appears to be promising for optimization of the bioelectrode response.

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Section: Resultsmentioning

confidence: 99%

“…Digital modeling of bienzyme bioelectrodes was summarized in 10. A specific dual bioelectrode with parallel substrates conversion was analyzed recently 11, but calculated results had not been compared with experimental ones.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Peroxidase‐Catalase Clark‐Type Biosensor: Computed and Experimental Response

et al. 2013

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“…Our simulation model relies on the following simplifications: the three free species, namely glucose, O 2 , and H 2 O 2 , obey Fick's diffusion laws, and reactions among them are isothermal. [ 29–31 ] GOx and PB NPs, about 50 nm in size, are alternately and uniformly distributed on the film with separation distances of about 100 nm based on SEM images. 2D cross‐section concentration profile is used to represent 3D concentration profile.…”

Section: Figurementioning

confidence: 99%

Mechanical Tolerance of Cascade Bioreactions via Adaptive Curvature Engineering for Epidermal Bioelectronics

et al. 2020

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Epidermal bioelectronics that can monitor human health status non‐invasively and in real time are core to wearable healthcare equipment. Achieving mechanically tolerant surface bioreactions that convert biochemical information to detectable signals is crucial for obtaining high sensing fidelity. In this work, by combining simulations and experiments, a typical epidermal biosensor system is investigated based on a redox enzyme cascade reaction (RECR) comprising glucose oxidase/lactate oxidase enzymes and Prussian blue nanoparticles. Simulations reveal that strain‐induced change in surface reactant flux is the key to the performance drop in traditional flat bioelectrodes. In contrast, wavy bioelectrodes capable of curvature adaptation maintain the reactant flux under strain, which preserves sensing fidelity. This rationale is experimentally proven by bioelectrodes with flat/wavy geometry under both static strain and dynamic stretching. When exposed to 50% strain, the signal fluctuations for wavy bioelectrodes are only 7.0% (4.9%) in detecting glucose (lactate), which are significantly lower than the 40.3% (51.8%) in flat bioelectrodes. Based on this wavy bioelectrode, a stable human epidermal metabolite biosensor insensitive to human gestures is further demonstrated. This mechanically tolerant biosensor based on adaptive curvature engineering provides a reliable bio/chemical‐information monitoring platform for soft healthcare bioelectronics.

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“…The schematic diagram of the system is provided in Figure 1. The above reaction in the enzymatic layer with the one-dimensional-in-space diffusion, described by Fick's law, leads to the following nonlinear transient reaction-diffusion equations [29] …”

Section: Isrn Electrochemistrymentioning

confidence: 99%

Theoretical Analysis of an Amperometric Biosensor Based on Parallel Substrates Conversion

Praveen¹,

Rajendran²

2014

ISRN Electrochemistry

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The behaviour of an amperometric biosensor based on parallel substrates conversion for steady-state condition has been discussed. This analysis contains a nonlinear term related to enzyme kinetics. Simple and closed form of analytical expressions of concentrations and of biosensor current is derived. This model was originally reported by Vytautas Aseris and his team (2012). Concentrations of substrate and product are expressed in terms of single dimensionless parameter. A new approach to Homotopy perturbation method (HPM) is employed to solve the system of nonlinear reaction diffusion equations. Furthermore, in this work, the numerical solution of the problem is also reported using Matlab program. The analytical results are compared with the numerical results. The analytical result provided is reliable and efficient to understand the behavior of the system.

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Modelling the biosensor utilising parallel substrates conversion

Cited by 14 publications

References 27 publications

Electrochemical Peroxidase‐Catalase Clark‐Type Biosensor: Computed and Experimental Response

Electrochemical Peroxidase‐Catalase Clark‐Type Biosensor: Computed and Experimental Response

Mechanical Tolerance of Cascade Bioreactions via Adaptive Curvature Engineering for Epidermal Bioelectronics

Theoretical Analysis of an Amperometric Biosensor Based on Parallel Substrates Conversion

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