Modelling of light-induced chlorophyll a fluorescence rise (O-J-I-P transient) and changes in 820 nm-transmittance signal of photosynthesis

Lazár, Dušan

doi:10.1007/s11099-009-0074-8

Cited by 79 publications

(67 citation statements)

References 64 publications

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“…The well-known mathematical models of the kinetic processes in PSII (Baake and Schlöder 1992;Stirbet et al 1998;Vredenberg 2000Vredenberg , 2011Stirbet et al 2001;Lazár 2003;Strasser et al 2004;Zhu et al 2005) describe only the fast OJIP induction phases of chlorophyll fluorescence on the time scale from milliseconds to hundreds of milliseconds (see review by Lazár 2009;Stirbet and Govindjee 2012). Further.…”

Section: Photosynth Resmentioning

confidence: 99%

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Thylakoid membrane model of the Chl a fluorescence transient and P700 induction kinetics in plant leaves

et al. 2016

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A new Thylakoid model is presented, which describes in detail the electron/proton transfer reactions between membrane protein complexes including photosystems II and I (PSII, PSI), cytochrome (Cyt) b f, mobile plastoquinone PQ pool in the thylakoid membrane, plastocyanin in lumen and ferredoxin in stroma, reduction of NADP via FNR and cyclic electron transfer. The Thylakoid model parameters were fitted both to Chl fluorescence induction data (FI) and oxido-reductions of P700 (ΔA) measured from 20 μs up to 20 s in pea leaves. The two-wave kinetics of FI and ΔA (O(JI)PSM and OABCDE) were described quantitatively, provided that the values of membrane electrochemical potential components ΔΨ(t), pH(t)/pH(t) are in physiologically relevant ranges. The time courses on the time scale from nanoseconds to tens of seconds of oxido-reduction changes of ET components as well as concentrations of proton/ions (K, Cl) were calculated. We assume a low constant FNR activity over this period. Charge movements across the thylakoid membrane by passive leakage and active ATPase transport and proton buffer reactions are simulated. The dynamics of charge fluxes during photosynthetic induction under low light (PFD 200 μmol photons m s) were analyzed. The initial wave of P700 oxidation within 20 ms during independent operation of PSI and PSII was followed after 50 ms by PSI donor-side reduction from reduced PQ pool via Cyt b f site. The Cyt b f reactions contribute to the stabilization of fluxes in the time range 1 s < t < 10 s. The detailed analysis of Chl a fluorescence at the PSM stage (t > 10 s) would need the investigation of FNR activation effect in order to explain the transitions between cyclic and linear electron transport.

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Section: Photosynth Resmentioning

confidence: 99%

“…Another model by Lazár (2009) includes the ET reactions in PSII (with the oxygen-evolving complex OEC), Cyt b 6 f, PSI, PQ pool in the membrane, PC in the lumen and Fd and FNR in the stroma. As a result, LEF and CEF chains in the thylakoid membrane were modeled, although proton fluxes in stroma and lumen were not considered explicitly.…”

Section: Photosynth Resmentioning

confidence: 99%

Thylakoid membrane model of the Chl a fluorescence transient and P700 induction kinetics in plant leaves

et al. 2016

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“…Most recently, mathematical modeling is used to attribute chlorophyll fluorescence rise to individual processes of energy flow through PS II and electron transport chain in thylakoid membrane of a chloroplast (e.g. Lazár 2006Lazár , 2009.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of Antarctic freshwater algae to salt stress assessed by fast chlorophyll fluorescence transient

Vilumbrales¹,

Skácelová²,

Barták³

2013

Czech Polar Rep.

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In this study, we investigated the effects of salt stress (2 mM NaCl) on excitation energy transfer from light harvesting complexes to photosystem II (PS II) in two Antarctic algal species: Klebsormidium sp. and Zygnema sp. Short-term salt stress led to a significant changes in the shape of chlorophyll fluorescence transient (OJIP). Analyses of the polyphasic fluorescence transients (OJIP) showed that the fluorescence yield at the phases J, I and P declined considerably with the time of exposition to salt stress. In both experimental species, OJIP transients reached lowest values of chlorophyll fluorescence signal after 30/60 min. of NaCl exposition. Then, OJIP shape and chlorophyll fluorescence showed species-specific recovery and rised towards original values (about 2/3 of untreated control). Analyses of chlorophyll fluorescence parameters derived from OJIPs showed that salt stress led to a decrease in the maximal efficiency of PS II photochemistry (F V /F M ) in Zygnema sp. but not Klebsormidium sp. The results indicated that the probability of excitation energy transfer before and beyond Q A , and the yield of electron transport beyond Q A is limited by salt-induced stress in Zygnema sp. In addition, salt stress resulted in a decrease in the photosynthetic electron transport per PS II reaction center, but both increase and decrease in the trapping per PS II reaction center was found. Performace index (PI abs ) was affected negatively in Zygnema sp. but possitively Klebsormidium sp. indicating that the latter species was more resistant to salt stress than Zygnema sp.

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“…At a DCMU concentration of 500 µM, the induction curve lost its sigmoid characteristic between steps J and I, since the fluorescence intensity in phase J was similar to F M ( Figures 1A and 1C), suggesting an accumulation of Q A -by the complete inhibition of the transfer of electrons from Q A to Q B (LAZAR, 2009). DCMU blocks the transfer of electrons from Q A to QB (VELTHUYS, 1981), as it competes with the Q B binding site on the D1 protein of PSII (TREBST; DRABER, 1986).…”

Section: Analysis Of Ojip Fluorescence Transient Kinetics and Jip Testmentioning

confidence: 99%

Chlorophyll fluorescence in the evaluation of photosynthetic electron transport chain inhibitors in the pea

Farias

Martinazzo

Bacarin

2016

Revista Ciência Agronômica

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-The study aimed to evaluate the behavior of the chair of photosynthetic electron transport in the presence of DCMU and atrazine in detached leaves of pea through simultaneous measurements of the kinetics of fluorescence transient, fluorescence delayed and modulated reflection at 820 nm. The petioles of the leaves were immersed for two hours in solution of inhibitors at concentrations of 0 (control), 25, 50, 100, 250 and 500 M. Measurements of the kinetics of fluorescence transient and fluorescence delayed and modulated reflection at 820 nm were recorded simultaneously by the M-PEA fluorometer. Simultaneous measurement of fluorescence transient, fluorescence delayed and modulated 820 nm reflection is important for assessments of the photosynthetic electron transport chain activity tool. The use of specific inhibitors of the electron transport chain allows you to collect and correlate a lot of information about the effect of different inhibitors at specific points in the photosynthetic electron transport chain. DCMU and atrazine are inhibitors of photosystem II and the concentration of 500 mM affects more strongly the flow of photosynthetic electrons. RESUMO -O trabalho teve como objetivo avaliar o comportamento da cadeia de transporte de elétrons fotossintética na presença de DCMU e atrazina, em folhas destacadas de ervilha, através de medidas simultâneas das cinéticas da fluorescência transiente, decaída da fluorescência da clorofila a e reflexão modulada a 820 nm. O pecíolo das folhas foi imerso por duas horas em solução de inibidores nas concentrações de 0 (controle), 25, 50, 100, 250 e 500 µM. Medidas da cinética da fluorescência transiente, da decaída da fluorescência e reflexão modulada a 820 nm foram gravadas, simultaneamente, pelo fluorômetro M-PEA. A medida simultânea da fluorescência transiente, decaída da fluorescência e reflexão modulada a 820 nm é ferramenta importante para avaliações da atividade da cadeia de transporte de elétrons fotossintética. O uso de inibidores específicos da cadeia de transporte de elétrons permite coletar e correlacionar uma série de informações sobre o efeito de diferentes inibidores em pontos específicos na cadeia de transporte de elétrons fotossintético. DCMU e atrazina são inibidores do fotossistema II e a concentração de 500 µM afeta de maneira mais acentuada o fluxo de elétrons fotossintético. Palavras-chave:Pisum sativum L.. Fluorescência transiente. Decaída da fluorescência. DCMU. Atrazina.

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Modelling of light-induced chlorophyll a fluorescence rise (O-J-I-P transient) and changes in 820 nm-transmittance signal of photosynthesis

Cited by 79 publications

References 64 publications

Thylakoid membrane model of the Chl a fluorescence transient and P700 induction kinetics in plant leaves

Thylakoid membrane model of the Chl a fluorescence transient and P700 induction kinetics in plant leaves

Sensitivity of Antarctic freshwater algae to salt stress assessed by fast chlorophyll fluorescence transient

Chlorophyll fluorescence in the evaluation of photosynthetic electron transport chain inhibitors in the pea

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