Modelling Mammary Metabolism in the Dairy Cow to Predict Milk Constituent Yield, with Emphasis on Amino Acid Metabolism and Milk Protein Production: Model Evaluation

Hanigan, M.D.; Crompton, L.A.; Bequette, B.J.; Mills, J.A.N.

doi:10.1006/jtbi.2002.3037

Cited by 44 publications

(38 citation statements)

References 54 publications

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“…Among the many AA essential for the lactating bovine mammary gland [13,28,31], methionine and lysine are considered the most important [12,32]. The AA in mammary gland in dairy cows are not solely used for protein synthesis, but also for production of energy through the Krebs cycle [12,33].…”

Section: Nutrition and Milk Protein Synthesismentioning

confidence: 99%

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Bionaz¹,

Hurley²,

Loor³

2012

Milk Protein

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Section: Nutrition and Milk Protein Synthesismentioning

confidence: 99%

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Bionaz¹,

Hurley²,

Loor³

2012

Milk Protein

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“…The utilization of AAs is important for mammary protein synthesis in lactating cows (Appuhamy et al, 2012;Arriola Apelo et al, 2014c). Previous research on AA metabolism primarily focused on optimizing balanced diets, which is essential for maintaining and enhancing milk protein synthesis in dairy cattle (Hanigan et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway

Gao

Zheng

et al. 2015

J. Zhejiang Univ. Sci. B

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Abstract:The aim of this study is to investigate the effects of leucine (Leu) and histidine (His) on the expression of both the mammalian target of rapamycin (mTOR) signaling pathway-related proteins and caseins in immortalized bovine mammary epithelial cells (CMEC-H), using a single supplement through Western blotting. The Earle's balanced salt solution (EBSS) was set as the control group and other treatment groups, based on the EBSS, were added with different concentrations of Leu or His, respectively. The results showed that, compared with the control group, the expression of caseins and the phosphorylation of mTOR (Ser Similarly, the His supplementation from 0.15 to 9.60 mmol/L increased the expression of αs2-casein, β-casein, κ-casein, P-mTOR (Ser 2481 ), P-Raptor (Ser 792 ), P-S6K1 (Thr 389 ), P-4EBP1 (Thr 37 ), P-eIF4E (Ser 209 ), and P-eEF2 (Thr 56 ) (P<0.01) in CMEC-H, whereas the αs1-casein expression was only reduced at 9.60 mmol/L His, G protein β subunit-like protein (GβL) at 0.15 and 9.60 mmol/L His, and P-RPS6 at 4.80 to 9.60 mmol/L His. Our linear regression model assay suggested that the αs1-casein expression was positively correlated with P-mTOR (P<0.01), P-S6K1 (P<0.01), and P-eEF2 (P<0.01) for the addition of Leu, while the expressions of β-casein (P<0.01) and κ-casein (P<0.01) were positively correlated with P-eEF2 for the addition of His. In conclusion, the milk protein synthesis was up-regulated through activation of the mTOR pathway with the addition of Leu and His in CMEC-H.

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“…lysine (Lys) have been identified frequently as the two mostlimiting AA for milk yield and milk protein synthesis (5,17).…”

mentioning

confidence: 99%

Ratio of lysine to methionine alters expression of genes involved in milk protein transcription and translation and mTOR phosphorylation in bovine mammary cells

Nan

et al. 2014

Physiological Genomics

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Modelling Mammary Metabolism in the Dairy Cow to Predict Milk Constituent Yield, with Emphasis on Amino Acid Metabolism and Milk Protein Production: Model Evaluation

Cited by 44 publications

References 54 publications

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway

Ratio of lysine to methionine alters expression of genes involved in milk protein transcription and translation and mTOR phosphorylation in bovine mammary cells

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