Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Bionaz, Massimo; Hurley, W.L.; Loor, Juan J.

doi:10.5772/46054

Cited by 60 publications

(108 citation statements)

References 188 publications

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“…83). Interestingly, the level of regulation of STAT5 through Jak/Stat signaling by hormones may vary across species, which has been reviewed previously (27). For instance, prolactin in rats increases before parturition and remains high during the entire lactation; in contrast in dairy cows this hormone increases sharply at the onset of lactation and decreases afterwards to prepartal levels.…”

Section: Transcription Factors Controlling Expression Of Casein Genesmentioning

confidence: 99%

“…For instance, prolactin in rats increases before parturition and remains high during the entire lactation; in contrast in dairy cows this hormone increases sharply at the onset of lactation and decreases afterwards to prepartal levels. Bionaz and collaborators (27) suggested that this difference between species might be associated to the high positive correlation between prolactin and suckling or milking, which is considerably higher in rodents than ruminants.…”

Section: Transcription Factors Controlling Expression Of Casein Genesmentioning

confidence: 99%

“…Among TF important for regulation of casein genes are the CCAAT/enhancer binding protein (C/EBP) beta, the glucocorticoid receptor Signal Transducer and Activator of Transcription 5 (STAT5), and the octamer binding transcription factor 1 with the Yin and Yang 1 protein being an inhibitor (27). Among them, STAT5 is the most important due to its role in controlling expression of various caseins, genes, and lactalbumin (27).…”

Section: Transcription Factors Controlling Expression Of Casein Genesmentioning

confidence: 99%

“…A review of the milk protein synthesis and potential regulation in dairy cows was recently published (27), and that information was used to construct the milk protein synthesis part in Fig. 1.…”

Section: Overview Of Milk Protein Synthesismentioning

confidence: 99%

“…Among them, STAT5 is the most important due to its role in controlling expression of various caseins, genes, and lactalbumin (27). Activated STAT5 binds to DNA sites known as GAS (interferon-gamma activated sequence) elements and upregulate target genes (reviewed in Ref.…”

Section: Transcription Factors Controlling Expression Of Casein Genesmentioning

confidence: 99%

See 4 more Smart Citations

Biosynthesis of milk fat, protein, and lactose: roles of transcriptional and posttranscriptional regulation

Osorio

Lohakare

Bionaz

2016

Physiological Genomics

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182

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Section: Transcription Factors Controlling Expression Of Casein Genesmentioning

confidence: 99%

Section: Transcription Factors Controlling Expression Of Casein Genesmentioning

confidence: 99%

Section: Transcription Factors Controlling Expression Of Casein Genesmentioning

confidence: 99%

Section: Overview Of Milk Protein Synthesismentioning

confidence: 99%

Section: Transcription Factors Controlling Expression Of Casein Genesmentioning

confidence: 99%

See 3 more Smart Citations

Biosynthesis of milk fat, protein, and lactose: roles of transcriptional and posttranscriptional regulation

Osorio

Lohakare

Bionaz

2016

Physiological Genomics

Self Cite

182

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Bovine mammary epithelial cells can grow and express milk protein synthesis genes at reduced fetal bovine serum concentration

Sattari,

Kjærup,

Rasmussen

et al. 2024

Cell Biology International

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Milk proteins produced by lactating cells isolated from bovine mammary tissue can offer a sustainable solution to the high protein demand of a global growing population. Serum is commonly added to culture systems to provide compounds necessary for optimal growth and function of the cells. However, in a cellular agricultural context, its usage is desired to be decreased. This study aims at examining the minimum level of fetal bovine serum (FBS) required for the growth and functionality of bovine mammary epithelial cells (MECs). The cells were isolated from dairy cows in early and mid‐lactation and cultured in reduced concentrations of FBS (10%, 5%, 1.25%, and 0%). Real‐time cell analysis showed a significant effect of lactation stage on growth rate and 5% FBS resulted in similar growth rate as 10% while 0% resulted in the lowest. The effect of reducing FBS on cell functionality was examined by studying the expressions of selected marker genes involved in milk protein and fat synthesis, following differentiation. The gene expressions were not affected by the level of FBS. A reduction of FBS in the culture system of MEC, at least down to 5%, does not assert any negative effect on the growth and expression levels of studied genes. As the first attempt in developing an in‐vitro model for milk component production using MEC, our results demonstrate the potential of MEC to endure FBS‐reduced conditions.

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Effect of supplementation of cows diet with linseed and fish oil and different variants of β‐lactoglobulin on fatty acid composition and antioxidant capacity of milk

et al. 2015

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Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Cited by 60 publications

References 188 publications

Biosynthesis of milk fat, protein, and lactose: roles of transcriptional and posttranscriptional regulation

Biosynthesis of milk fat, protein, and lactose: roles of transcriptional and posttranscriptional regulation

Bovine mammary epithelial cells can grow and express milk protein synthesis genes at reduced fetal bovine serum concentration

Effect of supplementation of cows diet with linseed and fish oil and different variants of β‐lactoglobulin on fatty acid composition and antioxidant capacity of milk

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