Modeling the microclimate inside a vessel in <i>in vitro</i> culture : vessel with agar

Abstract

Numerical simulations show that variations in vessel internal humidity was sensitive to transfer coefficient, climatic conditions within the growth chamber, evaporation and condensation of water vapor on the walls of the vessel. The variations in water vapor pressure deficits (VPD) (low during the nyctiperiod and high during the photoperiod) were well explained by a free convection model, which took into account temperature differences. Finally, the results show the necessity to construct containers with variable openings and growth chambers in which bioclimatic conditions can be controlled.Keywords : Agar, in vitro culture, microclimate, modeling, transfer coefficient. Résumé