Modeling Stress and Anxiety in Zebrafish

Cachat, Jonathan; Canavello, Peter R.; Elegante, Marco; Bartels, Brett; Elkhayat, Salem; Hart, Peter C.; Tien, Anna K.; Tien, David; Beeson, Esther; Mohnot, Sopan; Laffoon, Autumn L.; Stewart, Adam; Gaikwad, Siddharth; Wong, Keith; Haymore, Whitlee A.M.; Kalueff, Allan V.

doi:10.1007/978-1-60761-922-2_3

Cited by 43 publications

(39 citation statements)

References 55 publications

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“…More research is therefore needed to determine if embryonic PCB exposure potentiates anxiety-related responses in zebrafish larvae. Likely targets mediating such an effect would include the habenula, a region involved in fear and anxiety (Agetsuma et al, 2010; Lee et al, 2010), its neurochemical pathways, particularly dopamine and serotonin, neurotransmitters which are both decreased following PCB-exposure, and the hypothalamus-pituitary-interrenal axis which is homologous to the human hypothalamus-pituitary-adrenal axis (Cachat et al, 2011). …”

Section: Discussionmentioning

confidence: 99%

Effects of embryonic exposure to polychlorinated biphenyls (PCBs) on larval zebrafish behavior

Lovato

Créton

Colwill

2016

Neurotoxicology and Teratology

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Developmental disorders such as anxiety, autism, and attention deficit hyperactivity disorders have been linked to exposure to polychlorinated biphenyls (PCBs), a ubiquitous anthropogenic pollutant. The zebrafish is widely recognized as an excellent model system for assessing the effects of toxicant exposure on behavior and neurodevelopment. In the present study, we examined the effect of sub-chronic embryonic exposure to the PCB mixture, Aroclor (A) 1254 on anxiety-related behaviors in zebrafish larvae at 7 days post-fertilization (dpf). We found that exposure to low concentrations of A1254, from 2 to 26 hours post-fertilization (hpf) induced specific behavioral defects in two assays. In one assay with intermittent presentations of a moving visual stimulus, 5 ppm and 10 ppm PCB-exposed larvae displayed decreased avoidance behavior but no significant differences in thigmotaxis or freezing relative to controls. In the other assay with intermittent presentations of a moving visual stimulus and a stationary visual stimulus, 5 ppm and 10 ppm PCB-exposed larvae had elevated baseline levels of thigmotaxis but no significant differences in avoidance behavior relative to controls. The 5 ppm larvae also displayed higher terminal levels of freezing relative to controls. Collectively, our results show that exposure to ecologically valid PCB concentrations during embryonic development can induce functional deficits and alter behavioral responses to a visual threat.

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Section: Discussionmentioning

confidence: 99%

Effects of embryonic exposure to polychlorinated biphenyls (PCBs) on larval zebrafish behavior

Lovato

Créton

Colwill

2016

Neurotoxicology and Teratology

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“…For example, numerous paradigms have been adapted from rodents and applied to zebrafish (Table 1), showing a striking similarity in reduced exploration, and increased thigmotaxis or freezing (Cachat et al 2010b; Maximino et al 2010a; Maximino et al 2010b; Gaikwad et al 2011; Levin et al 2007). In addition, zebrafish anxiety research has the advantage of using both adults and larvae, complementing the throughput of larval models with the complexity of neural phenotypes of adult animals (Norton and Bally-Cuif 2010a; Stewart et al 2010a; Egan et al 2009; Grossman et al 2010; Burne et al 2011; Cachat et al 2010b; Webb et al 2009). Zebrafish also display robust behavioral phenotypes, which are exhibited through overt and easily quantified behavioral endpoints.…”

Section: Introductionmentioning

confidence: 99%

Modeling anxiety using adult zebrafish: A conceptual review

et al. 2012

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Zebrafish (Danio rerio) are rapidly emerging as a useful animal model in neurobehavioral research. Mounting evidence shows the suitability of zebrafish to model various aspects of anxiety-related states. Here, we evaluate established and novel approaches to uncover the molecular substrates, genetic pathways and neural circuits of anxiety using adult zebrafish. Experimental approaches to modeling anxiety in zebrafish include novelty-based paradigms, pharmacological and genetic manipulations, as well as innovative video-tracking, 3D-reconstructions and bioinformatics-based searchable databases and omics-based tools. Complementing traditional rodent models of anxiety, we provide a conceptual framework for the wider application of zebrafish and other aquatic models in anxiety research.

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“…Stress response and by inference anxiety have been widely studied in zebrafish in recent years and a variety of well-designed test methods have been developed [see recent reviews: (Cachat et al, 2010; Gerlai, 2010; Maximino et al, 2010)]. Useful test paradigms include shoaling response, predator avoidance and light-dark environment choice (Engeszer et al, 2004 Miller and Gerlai, 2007; Bass and Gerlai, 2008; Egan et al, 200 ; Gerlai et al, 2009; Champagne et al, 2010 Sackerman et al, 2010) in addition to the novel tank diving task described in this article.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish assessment of cognitive improvement and anxiolysis: filling the gap betweenin vitroand rodent models for drug development

Levin

2011

Revneuro

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Zebrafish can provide a valuable animal model to screen potential cognitive enhancing and anxiolytic drugs. They are economical and can provide a relatively quick indication of possible functional efficacy. In as much as they have a complex nervous system and elaborate behavioral repertoire, zebrafish can provide a good intermediate model between in vitro receptor and cell-based assays and classic mammalian models for drug screening. In addition, the variety of molecular tools available in zebrafish makes them outstanding models for helping to determine the neuromolecular mechanisms for psychoactive drugs. However, to use zebrafish as a translational model we must have validated, sensitive and efficient behavioral tests. In a series of studies, our lab has developed tests of cognitive function and stress response, which are sensitive to drug effects in a similar manner as rodent models and humans for cognitive enhancement and alleviating stress response. In particular, the three-chamber task for learning and memory was shown to be sensitive to the cognitive enhancing effects of nicotine and has been useful in helping to determine neural mechanisms crucial for nicotinic-induced cognitive enhancement. The novel tank diving test was shown to be a valid and efficient test of stress response. It is sensitive to the reduction of stress-related behaviors of the anxiolytic drugs diazepam and buspirone but not chlordiazepoxide. Nicotine also causes stress alleviating effects which can be interpreted as anxiolytic effects. Zebrafish models of behavioral pharmacology can be useful to efficiently screen test compounds for drug development and can be useful for helping to determine the mechanisms crucial for new therapeutic treatments of neurobehavioral impairments.

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Modeling Stress and Anxiety in Zebrafish

Cited by 43 publications

References 55 publications

Effects of embryonic exposure to polychlorinated biphenyls (PCBs) on larval zebrafish behavior

Effects of embryonic exposure to polychlorinated biphenyls (PCBs) on larval zebrafish behavior

Modeling anxiety using adult zebrafish: A conceptual review

Zebrafish assessment of cognitive improvement and anxiolysis: filling the gap betweenin vitroand rodent models for drug development

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