Modeling seizure-related behavioral and endocrine phenotypes in adult zebrafish

Wong, Keith; Stewart, Adam; Gilder, Thomas; Wu, Nadine; Frank, Kevin; Gaikwad, Siddharth; Suciu, Christopher; DiLeo, John; Utterback, Eli; Chang, Katie; Grossman, Leah; Cachat, Jonathan; Kalueff, Allan V.

doi:10.1016/j.brainres.2010.06.012

Cited by 112 publications

(70 citation statements)

References 62 publications

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“…Large clutch sizes, transparent larval stages, and the ability to perform forward or reverse genetic analyses have particularly facilitated the use of embryonic and larval zebrafish (Lieschke and Currie 2007). Mature zebrafish, which possess well-developed endocrine, sensory, and motor systems (Bally-Cuif and Vernier 2010), a range of complex behaviours such as social interactions (Saverino and Gerlai 2008), learning and memory (Gomez-Laplaza and Gerlai 2010; Norton and Bally-Cuif 2010;Al-Imari and Gerlai 2008) and a physiology similar to mammals (Bally-Cuif and Vernier 2010), have been recognized as beneficial to investigations of neurological diseases (Alfaro et al 2011;Siebel et al 2011;Wong et al 2010;Keller and Murtha 2004;Mathur and Guo 2010;Ramirez et al 2012;Haud et al 2011;Williams et al 2011). As adult zebrafish continue to be used as an experimental model, new techniques are being developed to better characterize their behaviour , neuronal activity (Pineda et al 2011), neurochemistry (Kabli et al 2009) and neuromorphology (Rao et al 2009;Ullmann et al 2010b;Ullmann et al 2010a; Kabli et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Enhanced characterization of the zebrafish brain as revealed by super-resolution track-density imaging

et al. 2013

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Abstract:In this study we explored the use of super-resolution track density imaging (TDI) for neuroanatomical characterization of the adult zebrafish brain. We compared the quality of image contrast and resolution obtained with T 2 * magnetic resonance imaging (MRI), diffusion tensor based imaging (DTI), TDI, and histology. The anatomical structures visualized in 5 µm TDI maps corresponded with histology. Moreover the super-resolution property and the local-directional information provided by directionally-encoded color TDI facilitated delineation of a larger number of brain regions, commissures and small white matter tracks when compared to conventional MRI and DTI. In total, we were able to visualize 17 structures that were previously unidentifiable using MR microimaging, such as the four layers of the optic tectum. This study demonstrates the use of TDI for characterization of the adult zebrafish brain as a pivotal tool for future phenotypic examination of transgenic models of neurological diseases.

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Section: Introductionmentioning

confidence: 99%

Enhanced characterization of the zebrafish brain as revealed by super-resolution track-density imaging

et al. 2013

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“…Corkscrew swimming (spiraling, whirling): Spiral swimming with an increased speed and in an uncoordinated direction; commonly observed as part of seizure phenotype. 43,103 1.38. Courtship: Complex patterned behaviors that precede spawning.…”

Section: Coloration Responsementioning

confidence: 99%

Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond

et al. 2013

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Zebrafish (Danio rerio) are rapidly gaining popularity in translational neuroscience and behavioral research. Physiological similarity to mammals, ease of genetic manipulations, sensitivity to pharmacological and genetic factors, robust behavior, low cost, and potential for high-throughput screening contribute to the growing utility of zebrafish models in this field. Understanding zebrafish behavioral phenotypes provides important insights into neural pathways, physiological biomarkers, and genetic underpinnings of normal and pathological brain function. Novel zebrafish paradigms continue to appear with an encouraging pace, thus necessitating a consistent terminology and improved understanding of the behavioral repertoire. What can zebrafish 'do', and how does their altered brain function translate into behavioral actions? To help address these questions, we have developed a detailed catalog of zebrafish behaviors (Zebrafish Behavior Catalog, ZBC) that covers both larval and adult models. Representing a beginning of creating a more comprehensive ethogram of zebrafish behavior, this effort will improve interpretation of published findings, foster cross-species behavioral modeling, and encourage new groups to apply zebrafish neurobehavioral paradigms in their research. In addition, this glossary creates a framework for developing a zebrafish neurobehavioral ontology, ultimately to become part of a unified animal neurobehavioral ontology, which collectively will contribute to better integration of biological data within and across species.

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“…Numerous studies have showed that adult or zebra-fish larvae exposed to PTZ exhibit increased locomotor activity, seizure-like behavior, and epileptiform electrographic activity [15,19] [ [22][23][24], which have a good correlation between zebra-fish and rodent data [15,17,21,25]. Also, many researchers have used zebra-fish seizure model for anti- convulsions in zebra-fish larvae was assessed through quantification of the high-speed moved distance.…”

Section: Discussionmentioning

confidence: 99%

Validation of the Zebra-fish Pentylene Tetrazol Seizure Model: Behaviour Assay for Assessing Anti-Epileptic Drug Efficacy

Liu¹,

Zhou²

2016

Biochem Anal Biochem

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The availability of zebra-fish larvae model of epileptic seizure provides opportunities to identify novel anticonvulsants for treatment of people with epilepsy. However, the major parameters of zebra-fish behavior assay for assessing antiepileptic drug efficacy existed disparity, which resulted in different results in previous studies. In this study, we chose the high, medium, slow-speed moved distances and the total distances moved for seizure-like activity quantification in individual wells of a 48-well plate in the dark phase and used this zebra-fish seizure model to assess three commonly prescribed anti-epileptic drug's efficacy and screen bioactive components from plant extract. Results showed that the high-speed moved distances were given a more reasonable and sensitive dose-response curve than the total distances in zebra-fish larvae model exposed to 10 mM pentylene tetrazole. Besides, we also optimized the vehicle (dimethyl sulfoxide, DMSO) concentration used in epileptic behaviour assay. The three anti-epileptic drugs, phenytoin, valproate sodium and carbamazepine showed the same efficacy patterns in zebra-fish seizure model as in mammalian epileptic models. We also found four positive hits from plant (Vaccinium spp.) extract in primary drug screening, two hits exhibited concentration-dependent inhibition of locomotor activity, confirming their anticonvulsant characteristics. These results indicated that this zebra-fish larvae model could be useful for assessing anti-epileptic drug efficacy, facilitating the primary drug screening and evaluating of effective components in medicinal plants.

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Modeling seizure-related behavioral and endocrine phenotypes in adult zebrafish

Cited by 112 publications

References 62 publications

Enhanced characterization of the zebrafish brain as revealed by super-resolution track-density imaging

Enhanced characterization of the zebrafish brain as revealed by super-resolution track-density imaging

Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond

Validation of the Zebra-fish Pentylene Tetrazol Seizure Model: Behaviour Assay for Assessing Anti-Epileptic Drug Efficacy

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