Modeling of Protein Adsorption in Membrane Affinity Chromatography

Wu, Hao; Chen, Zhaoan; Wang, Junde; Li, Xueliang

doi:10.1081/al-120035901

Cited by 8 publications

(1 citation statement)

References 23 publications

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“…Furthermore, compared to packed columns, membranes exhibit improved mass-transfer efficiencies, higher throughput values due to the presence of macropores that enable primarily convective flow, no intra-pore diffusion and compactness of bed, a better accessibility of ligands for affinity interactions, a higher dynamic binding capacity (by more than one order of magnitude) (Teeters et al, 2003) and easier scalingup. The applications of affinity membranes in biotechnology are rapidly growing, as demonstrated in numerous review articles (Brandt et al, 1988;Roper and Lightfoot, 1995;Thommes and Kula, 1995;Charcosset, 1998, Klein, 1990, 2000Ghosh, 2002Suen et al, 2003;Hao et al, 2004;Borcherding et al, 2003;Zeng and Ruckstein, 1999;Piletsky et al, 1999).…”

Section: Affinity Membranesmentioning

confidence: 99%

Separation Media in Affinity Chromatography of Proteins - A Critical Review

Varilova¹,

Madera²,

Pacáková³

et al. 2006

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The rapid development of proteomics has been made possible primarily by the progress in analytical and preparative separation methods. Biological systems are so complex that the separation procedures employed must be highly efficient as well as highly selective. The latter requirement is best met by affinity separations based on molecular recognition. The present review critically discusses the properties of the affinity sorbents employed in proteomics, namely, agarose gels, dextrans, modified methacrylate and acrylamide polymers, porous and nonporous silica, porous glass beads, monoliths, affinity membranes and magnetic beads. The physico-chemical properties of these materials, their preparation, application, approaches to their modification and their relative advantages and drawbacks are discussed.

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Section: Affinity Membranesmentioning

confidence: 99%

Separation Media in Affinity Chromatography of Proteins - A Critical Review

Varilova¹,

Madera²,

Pacáková³

et al. 2006

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Investigation of the concentration dependence of mass transfer coefficients in reversed-phase liquid chromatography

Wang

Zhang

2006

J. Sep. Sci.

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In order to investigate the concentration dependence of mass transfer coefficients in RPLC, experimental breakthrough curves obtained by staircase frontal analysis (FA) were fitted to the simplified models such as multiplate (MP) model, equilibrium dispersive (ED) model, and transport model, and the sophisticated models such as lumped pore diffusion (POR) model and general rate (GR) model. The MP model was used to obtain the initial guesses of the parameters of the ED and the transport models. Then the best values were obtained by minimizing the differences between theoretical and experimental values with a nonlinear fitting procedure. The values of the parameters of the POR and the GR models can be calculated by using the expressions derived from the plate height equations, which was further validated by using the fitting method. It was found that the mass transfer coefficients would depend on the solute concentration. This can be ascribed to the surface diffusivity, which correlates with the concentration and is lumped into the mass transfer coefficients for both simplified and sophisticated models.

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Conversion of paper to film by ionic liquids: manufacturing process and properties

et al. 2018

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8In this study, we investigate the "chemical welding" of paper with the ionic liquid (IL) 1-ethyl-3-9 methylimidazolium acetate ([EMIM]OAc) using a two-step process. First, the IL is transported into 10 the structure of the paper as a water solution. Then, partial dissolution is achieved by activation 11 with heat, where the water evaporates and the surfaces of the fibres partially dissolve. The 12 activated paper is washed with water to remove IL, and dried to fuse fibre surfaces into each 13 other. The "chemically welded" paper structure has both elevated dry and wet strength. The 14 treatment conditions can be adjusted to produce both paper-like materials and films. The most 15 severe treatment conditions produce films that are fully transparent and their oxygen and grease 16 2 barrier properties are excellent. As an all-cellulose material, the "chemically welded" paper is 17 fully biodegradable and is a potential alternative to fossil fuel-based plastics. 18 INTRODUCTION 19The consumption of packaging materials has continuously been rising due to increased 20 transportation of goods and food materials around the world, which has been steadily increased 21 due to online shopping and offshoring of manufacturing plants. 1 This rise in material 22 consumption has been met mainly by fossil fuel-based plastics and lignocellulose-based 23 materials. Since packages are typically utilized only once, plastic materials, which have 24 incomplete recycling cycles, cause environmental pollution, also harming animals and other living 25 organisms, including, for example, human health issues related to the microplastics problem. 2 26Consequently, the choice of packaging materials has been guided towards biodegradable, 27 renewable materials. Thus, much of the growth in packaging material consumption has been 28 covered by lignocellulosic materials, due to the renewability, recyclability, and abundance of 29 wood fibre materials. 3 Among wood-based materials, cardboard, wrapping papers and films have 30 been the most important materials used in packaging applications. 31Wood fibres are mostly composed of cellulose, a linear polymer composed of glucose units 32 connected by glycosidic bonds. 4 In paper and board, fibres are connected by interfibrillar 33 hydrogen bonds forming layered sheets. Paper is strong when dry, but easily disintegrates in 34 water, because water effectively breaks those bonds. 5 Therefore, in many paper applications, 35 some wet strength additives are used to increase the water tolerance of paper via 36 hydrophobization or forming chemical linkages between fibres. 5 Crystalline cellulose has an 37Corresponding Author Author Contributions 344The manuscript was written through contributions of all the authors. All authors have given approval to the final version of the 345 manuscript. 346 347 348 349 350 351 352 Highlights 412• Two-step partial dissolution reaction with [EMIM]OAc was used to transform paper to 413 permanent all-cellulose material. 414• Water was used to ease the penetration of [EMIM]OAc...

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Modeling of Protein Adsorption in Membrane Affinity Chromatography

Cited by 8 publications

References 23 publications

Separation Media in Affinity Chromatography of Proteins - A Critical Review

Separation Media in Affinity Chromatography of Proteins - A Critical Review

Investigation of the concentration dependence of mass transfer coefficients in reversed-phase liquid chromatography

Conversion of paper to film by ionic liquids: manufacturing process and properties

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