Modeling of microwave-assisted extraction of natural dye from seeds of Bixa orellana (Annatto) using response surface methodology (RSM) and artificial neural network (ANN)

Sinha, Keka; Chowdhury, Shamik; Das, Papita; Datta, Siddhartha

doi:10.1016/j.indcrop.2012.04.004

Cited by 163 publications

(59 citation statements)

References 28 publications

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“…In practice, annatto dye in specific concentrations (2,4,8,16, 32 and 64 mg/mL) was mixed with sterile Mueller Hinton agar then 0.1 mL of bacterial suspension (0.5 McFarland) was plated as pour-plat. Plates were maintenance at 37°C and the MIC of bacteria was evaluated after 18 -24 hours of incubation [17].…”

Section: Methodsmentioning

confidence: 99%

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Comparing Antibacterial and Antioxidant Activity of Annatto Dye Extracted by Conventional and Ultrasound-Assisted Methods

Yolmeh

Najafi

Shakouri

et al. 2015

Zahedan J Res Med Sci

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Background: Annatto dye is used extensively in food industry that has antibacterial and antioxidant properties. Objectives: Aim of this paper was comparison of the antibacterial and antioxidant properties of annatto dye was extracted by conventional and ultrasound-assisted extraction (UAE) methods. Materials and Methods:In this experimental study minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of annatto dye against the bacteria were determined by agar dilution method. Antioxidant activity of annatto dye was evaluated by DPPH method. Results: Gram-positive bacteria show more sensitivity to annatto dye than Gram-negative bacteria. Annatto dye extracted by UAE has more antibacterial and antioxidant activity compared to conventional method. Bacillus subtilis and Escherichia coli have the highest and the lowest sensitivity to annatto dye, respectively. Conclusions: Annatto dye extracted by UAE showed a bactericidal effect against Salmonella enteritidis, however the dye extracted by conventional method the opposite is true.

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Section: Methodsmentioning

confidence: 99%

“…Simultaneous with the increasing awareness of toxicity of synthetic colors, need for colorants from natural sources has increased [1,2]. The natural colors that are often called biocolors, because of their biological source usually extracted from vegetables, fruits, roots, and microorganisms [3].…”

Section: Introductionmentioning

confidence: 99%

Comparing Antibacterial and Antioxidant Activity of Annatto Dye Extracted by Conventional and Ultrasound-Assisted Methods

Yolmeh

Najafi

Shakouri

et al. 2015

Zahedan J Res Med Sci

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show abstract

“…The reason for the increment of yield could be due to better leaching of natural dye material from plant cell membranes and mass transfer to solvent assisted by microwave (electromagnetic) energy provided by microwave. A study has reported that microwave enhances the mass transfer of colouring matter from natural plant material and transport to the solvent medium [14].…”

Section: Resultsmentioning

confidence: 99%

Comparisons between Conventional and Microwave-Assisted Extraction of Natural Colorant From Mesocarp and Exocarp of Cocus Nucifera

Hassan¹,

Zulrushdi²,

Yusoff³

et al. 2015

JMSE-B

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This study aimed at exploring the feasibility of using the mesocarp and exocarp of Cocus nucifera as a natural colorant using microwave-assisted extraction in which often regarded as a waste materials. The effects of microwave power and heating time on the yield of colorant, pH and colour determination were investigated. The highest colorant yield in mesocarp and exocarp was found to be 6.0% (8 min of extraction at 100 W) and 6.8% (12 min of extraction at 300W), respectively. While the control, extraction of colorant by conventional method (90 min at 90 º C), showed a percentage of 5.2% (mesocarp) and 3.6% (exocarp) yield of colorant. Meanwhile, pH of all extracts fluctuated throughout the experiment. The results proved that heating time and microwave power slightly affected the pH of all samples due to pH being inconsistent throughout the experiment. In colour determination, lightness (L*) was slightly decreased for all samples which extracted at all microwave power which indicated that the longer the heating time the darker colour was extracted. Overall, the colour of exocarp was darker than mesocarp extract due to L*, c* and h* values were lower than the mexocarp extract. The utilization of microwave-assisted extraction has significant improvement in the extraction of natural colorant compared to traditional boiling method. In fact, application of microwave irradiation method proved to be a rapid and improved technique for natural colorant extraction and extensively reduced the extraction time.

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“…The seed preparation and extraction process were carried out by performing different sets of experiments and also by modifying those methods reported in the literature [12][13][14][15]. The seeds were pulped out from the capsule and were allowed to dry in an oven at 40°C for 24 h. Dried seed samples were initially soaked in hexane for 6 h to remove fats and waxes.…”

Section: Sample Preparation and Extractionmentioning

confidence: 99%

Isolation, Characterization and Biological Activities of Food Colorants from Bixa orellana

Melka¹,

Bisrat²,

G³

2017

J Pharmacovigil

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Modeling of microwave-assisted extraction of natural dye from seeds of Bixa orellana (Annatto) using response surface methodology (RSM) and artificial neural network (ANN)

Cited by 163 publications

References 28 publications

Comparing Antibacterial and Antioxidant Activity of Annatto Dye Extracted by Conventional and Ultrasound-Assisted Methods

Comparing Antibacterial and Antioxidant Activity of Annatto Dye Extracted by Conventional and Ultrasound-Assisted Methods

Comparisons between Conventional and Microwave-Assisted Extraction of Natural Colorant From Mesocarp and Exocarp of Cocus Nucifera

Isolation, Characterization and Biological Activities of Food Colorants from Bixa orellana

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