“…When in an immobilized form, it is assumed that the matrix does not significantly alter the internal pH environment. The assumption is justified by the fact that there is, in general, a buffer present in the biological system, which facilitates the transport of hydrogen ions and thus decreases the pH difference between the matrix and the bulk solution (Byers et al, 1992;Carleysmith et al, 1980;Engasser and Horvath, 1974;Ogundiran et al, 1991;Ruckenstein and Rajora, 1985;Ruckenstein and Varanasi, 1984;Varanasi et al, 1987). With the bulk solution being acidic to favor enzyme E 1 , enzyme E 2 , which has a basic optimal pH, must be immobilized.…”