Comparison of label-free ACh-imaging sensors based on CCD and LAPS

Werner, Carl Frederik; Takenaga, Shoko; Taki, Hidenori; Sawada, Kazuaki; Schöning, Michael J.

doi:10.1016/j.snb.2012.11.012

Cited by 19 publications

(10 citation statements)

References 39 publications

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“…One of the most familiar semiconductor‐based chemical sensors is the ion‐sensitive field‐effect transistor (ISFET), which was introduced in 1970, and much fundamental research has been performed to apply it finally in food control, environmental monitoring, as well as biological measurements . Based on the same principle, the light‐addressable potentiometric sensor (LAPS) has been developed as semiconductor‐based chemical sensor, which can detect, e.g., the local hydrogen concentration or other ion concentrations (e.g., Li + , Na + , K + , Ca 2+ , Cu 2+ , and Cd 2+ ) and chemical species (e.g., glucose, penicillin, and acetylcholine) in solution, by using an appropriate transducer structure for the sensor surface. In addition, LAPS has been recently developed as a semiconductor‐based biosensor for the monitoring of the metabolism of Chinese hamster ovary (CHO) cells by detecting the release of H + ions .…”

Section: Introductionmentioning

confidence: 99%

Fabrication of biocompatible lab‐on‐chip devices for biomedical applications by means of a 3D‐printing process

Takenaga

Schneider

Erbay

et al. 2015

Physica Status Solidi (a)

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A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.

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Section: Introductionmentioning

confidence: 99%

Fabrication of biocompatible lab‐on‐chip devices for biomedical applications by means of a 3D‐printing process

Takenaga

Schneider

Erbay

et al. 2015

Physica Status Solidi (a)

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“…ml/h, (c) 1 ml/h [27] LAPS analysis system can also realize visual analysis of neurotransmitters such as acetylcholine. Werner et al constructed a miniature LAPS analysis system by modifying the surface of LAPS with acetylcholinase, and realized the visual analysis of acetylcholine [28] . Similarly, a CCD based pH image sensor can also be used for visual analysis of acetylcholine [29] .…”

Section: Enzymatic Reactionmentioning

confidence: 99%

Light-Addressable Potentiometric Sensors in Microfluidics

li¹,

liu²,

Tan³

et al. 2021

Preprint

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Light-addressable potentiometric sensor (LAPS) is an electrochemical sensor based on the field-effect principle of semiconductor. It is able to sense the change of Nernst potential on the sensor surface, and the measuring area can be controlled by the illumination. Due to the unique light-addressable ability of LAPS, the chemical imaging system constructed with LAPS can realize the two-dimensional image distribution detection of chemical/biomass. In this paper, the advantages of LAPS as sensing unit of microelectrochemical analysis system are summarized. Then, the greatest development of LAPS analysis system is explained and discussed. Especially, this paper focused on the research of ion diffusion, enzymatic reaction, microbial metabolism and droplet microfluidics by using LAPS analysis system. Finally, the development trends and prospects of LAPS analysis system are illustrated.

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“…The detection of inorganic salt ions (e.g., K + , Ca 2+ , Na + , Mg 2+ , Zn 2+ , Li + , NO 3 − , CO 3 2− , and SO 4 2− ) can be achieved by depositing polymer membranes carrying ionophores such as PVC membranes [53,70,71,72,73,74,75,76], silicone rubber membranes [77,78,79,80,81] and photocurable membranes [82]. Immobilization of enzymes [83,84,85,86,87,88,89,90], antibodies [23,91,92] or DNA [93,94,95,96] on the sensing layer enables the detection of biomolecules such as glucose, urea, penicillin G, antigens, immunoglobulins G (IgG) and target DNA, etc. The sensing materials and target species have been systematically summarized [20,21,22].…”

Section: Measurement System Set-upmentioning

confidence: 99%

Recent Developments of High-Resolution Chemical Imaging Systems Based on Light-Addressable Potentiometric Sensors (LAPSs)

Liang

Qiu

Gan

et al. 2019

Sensors

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A light-addressable potentiometric sensor (LAPS) is a semiconductor electrochemical sensor based on the field-effect which detects the variation of the Nernst potential on the sensor surface, and the measurement area is defined by illumination. Thanks to its light-addressability feature, an LAPS-based chemical imaging sensor system can be developed, which can visualize the two-dimensional distribution of chemical species on the sensor surface. This sensor system has been used for the analysis of reactions and diffusions in various biochemical samples. In this review, the LAPS system set-up, including the sensor construction, sensing and substrate materials, modulated light and various measurement modes of the sensor systems are described. The recently developed technologies and the affecting factors, especially regarding the spatial resolution and temporal resolution are discussed and summarized, and the advantages and limitations of these technologies are illustrated. Finally, the further applications of LAPS-based chemical imaging sensors are discussed, where the combination with microfluidic devices is promising.

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Comparison of label-free ACh-imaging sensors based on CCD and LAPS

Cited by 19 publications

References 39 publications

Fabrication of biocompatible lab‐on‐chip devices for biomedical applications by means of a 3D‐printing process

Fabrication of biocompatible lab‐on‐chip devices for biomedical applications by means of a 3D‐printing process

Light-Addressable Potentiometric Sensors in Microfluidics

Recent Developments of High-Resolution Chemical Imaging Systems Based on Light-Addressable Potentiometric Sensors (LAPSs)

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