Modeling of Chlorophyll a Fluorescence Kinetics in Plant Cells: Derivation of a Descriptive Algorithm

On the quantitative relation between dark kinetics of NPQ-induced changes in variable fluorescence and the activation state of the CF₀.CF₁.ATPase in leaves

“…The pmf (Cruz et al 2001) is, characteristic for a current generating device, linear related to the pump-generated proton flux Φ H + , and inversely dependent on the membrane proton conductance Our data on the characteristics of the dark kinetics of F v after a low intensity (0.05k) pulse are in harmony with the hypothesis that a major part of the variable fluorescence F v in these pulses is caused by changes in photoelectric events in the photosynthetic membrane and associated with active trans-membrane proton pumping into the chloroplast lumen. The effect of the interaction of an electric field, in this case associated with the pmf, with the energy levels in the RC on the Chl fluorescence has been amply discussed (van Grondelle 1985, Bulychev and Vredenberg 2001, Vredenberg and Prášil 2009. Our fluorescence decay kinetics (Figs.…”

“…Kinetic aspects of electron transport in PSI and PSII in chloroplasts and intact leaves have fruitfully been studied by monitoring kinetics of light-induced 810-nm absorbance changes (ΔA 810 ), attributed to redox changes of P700 (Harbinson and Hedley 1993, Vredenberg and Bulychev 2010, Schreiber and Klughammer 2016, and of chlorophyll a (Chl) fluorescence induction responses, respectively (Stirbet et al 1998, Vredenberg 2000, Papageorgiou and Govindjee 2004, Lazár and Schansker 2009, Vredenberg and Prášil 2009, Belyaeva et al 2016. Recently light-dependent absorbance changes in the 510-535-nm wavelength region attributed to electrochromic band shifts of carotenoids (P515) have received and regained vivid attention.…”

Chlorophyll a fluorescence induction: a personal perspective of the thermal phase, the J–I–P rise

“…This can be done by using models based on generally accepted structural and functional information (e.g., biological, biochemical and biophysical) obtained from specific samples (e.g., leaf, individual cells, intact chloroplasts, thylakoids). This criterion, of what might be called 'structure-based modeling', is not fulfilled in some studies, in which the experimental curves were fitted with analytical functions that were not deduced from structural information (see e.g., Dau 2000, 2002;Boisvert et al 2006;Antal and Rubin 2008;Joly and Carpentier 2009; and also, partially, in the models of Vredenberg 2008a, Vredenberg and Prášil 2009, Vredenberg 2011.…”

“…Moreover, Vredenberg and coworkers have also presented a mathematical model, called 'Fluorescence Induction Algorithm' (FIA), in which an extended version of TSTM has been applied. They have simulated the O-J-I-P transient with FIA (Vredenberg 2008a(Vredenberg , 2011Vredenberg and Prášil 2009) …”

Modelling of light-induced chlorophyll a fluorescence rise (O-J-I-P transient) and changes in 820 nm-transmittance signal of photosynthesis

“…Even if simulations of the FLR based on models which included electron transport reactions occurring beyond PQ pool have been published, too (Lebedeva et al 2002, Kroon and Thoms 2006, Laisk et al 2006, the papers did not deal with description of the effect of the reactions on the FLR and they also did not simulate the I 820 signal paralleling the FLR. For more details on modelling of the Chl a FLR see recent reviews by Lazár and Schansker (2009), Rubin and Riznichenko (2009) and Vredenberg and Prášil (2009).…”