Modeling and Optimizing in vitro Sterilization of Chrysanthemum via Multilayer Perceptron-Non-dominated Sorting Genetic Algorithm-II (MLP-NSGAII)

Hesami, Mohsen; Naderi, Roohangiz; Tohidfar, Masoud

doi:10.3389/fpls.2019.00282

Cited by 55 publications

(60 citation statements)

References 58 publications

(110 reference statements)

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“…However, in J. bufonius, both AgNO 3 and Ca(ClO) 2 caused explant death, unlike NaClO (control), which had a survival percentage of 80%. The efficiency of AgNO 3 as a disinfection solution prior to the establishment of explants in vitro may be limited due to the instability of AgNO 3 in the presence of chemical components that may adhere to or be exuded by the explants or compounds remaining from the processes of washing with tap water, such as chlorides or other salts 36 . Thus, the results indicate that continuing to use the methodology previously established in the Biotechnology and Environmental Studies Laboratory (NaClO at 7.4 mg mL −1 for 20 min) is recommended for J. bufonius.…”

Section: Discussionmentioning

confidence: 99%

Advances of native and non-native Antarctic species to in vitro conservation: improvement of disinfection protocols

Cuba‐Díaz

Rivera-Mora

Navarrete

et al. 2020

Sci Rep

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Plants that inhabit Antarctica have raised scientific interest due to their resilience to climate change, abiotic tolerance mechanisms and potential biological applications. In vitro propagation is useful for conservation, genetic material availability of these species and avoiding mass collection in their habitat. In vitro culture protocols for the native plants Colobanthus quitensis and Deschampsia antarctica and the non-native Juncus bufonius have been affected by endophytic microorganisms that proliferate when introduced to tissue cultures. This study evaluated the microbicidal and phytotoxic effect of calcium hypochlorite (ca(clo) 2), silver nitrate (AgNO 3) and silver nanoparticles (AgNPs), and their use at different concentrations for different time periods. The Ca(ClO) 2 at 100 mg mL −1 showed the best microbial contamination control in D. antarctica (applied for 20 min) and for the three C. quitensis populations (applied for 15 min). In J. bufonius, AgNO 3 at 10 mg mL −1 for 10 min reduced the microbial growth, but oxidative damage was generated. AgNPs did not prevent contamination or have adverse effects on tissues. Survival plantlets from each treatment, population or species were effectively introduced to the tissue culture and their propagation was successful. these results constitute a fundamental advance for the introduction, propagation and conservation of Antarctic species and their use in scientific research. Global loss of biodiversity has increased considerably in recent years, which has made implementing conservation strategies essential for preserving and propagating plant genetic resources in each geographical region 1-3. One method of conservation is through the use of germplasm banks, or facilities and centers created to conserve genetic resources under favorable conditions to prolong their survival; the final objective is the ex-situ conservation of specific genetic diversity. They are a source of material that enables many possibilities for a species of interest, from developing new cultivable varieties, biological technologies, or to establishing ecological restoration plans 4. The Antarctic continent is an extreme ecosystem due to its adverse environmental conditions. Its organisms are exposed to restricted availability of water and nutrients, very low temperatures, frequent freezing and thawing cycles, prolonged periods of darkness in the winter and exposure to high UV radiation levels during summer. These environmental characteristics are inhospitable to most organisms that live in temperate areas 5. In addition, the increase in human activity in the region, and the confirmation that Antarctica is one of the critical points of global warming, make it a continent of highest priority for the conservation and development of multinational and multidisciplinary scientific challenges 6,7. There are only two native flowering plants that have been able to colonize some ice-free areas in the Antarctic Peninsula: Deschampsia antarctica Desv. (Poaceae) and Colobanthus quitensis (Kunth.) Bartl....

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Section: Discussionmentioning

confidence: 99%

Advances of native and non-native Antarctic species to in vitro conservation: improvement of disinfection protocols

Cuba‐Díaz

Rivera-Mora

Navarrete

et al. 2020

Sci Rep

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“…Recently, different machine learning algorithms have been successfully utilized to predict and optimize various plant physiological processes. Several studies [24,58,63] used MLP to predict various plant biological processes. However, they only applied the MLP model and did not compare this well-known algorithm with other models.…”

Section: Plos Onementioning

confidence: 99%

The application of artificial neural networks in modeling and predicting the effects of melatonin on morphological responses of citrus to drought stress

Jafari

Shahsavar

2020

PLoS ONE

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Drought stress as one of the most devastating abiotic stresses affects agricultural and horticultural productivity in many parts of the world. The application of melatonin can be considered as a promising approach for alleviating the negative impact of drought stress. Modeling of morphological responses to drought stress can be helpful to predict the optimal condition for improving plant productivity. The objective of the current study is modeling and predicting morphological responses (leaf length, number of leaves/plants, crown diameter, plant height, and internode length) of citrus to drought stress, based on four input variables including melatonin concentrations, days after applying treatments, citrus species, and level of drought stress, using different Artificial Neural Networks (ANNs) including Generalized Regression Neural Network (GRNN), Radial basis function (RBF), and Multilayer Perceptron (MLP). The results indicated a higher accuracy of GRNN as compared to RBF and MLP. The great accordance between the experimental and predicted data of morphological responses for both training and testing processes support the excellent efficiency of developed GRNN models. Also, GRNN was connected to Non-dominated Sorting Genetic Algorithm-II (NSGA-II) to optimize input variables for obtaining the best morphological responses. Generally, the validation experiment showed that ANN-NSGA-II can be considered as a promising and reliable computational tool for studying and predicting plant morphological and physiological responses to drought stress.

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“…In vitro propagation of plants is a useful and efficient technique that enables mass production of virus-free plants (Hesami et al, 2017a;Hesami et al, 2019a). Tissue and organ cultures have been widely used for the mass production of various plants (Hesami and Daneshvar, 2016a;Hesami et al, 2019b). Regarding the application of tissue culture techniques for the propagation of ornamental and medicinal plants, most studies have focused on two goals: mass production of plants and production of disease-free plants Jafari et al, 2017;Jafari et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Effects of thidiazuron on in vitro shoot regeneration of Morus alba

Rezaei-Zafarghandi¹,

Rahmati‐Joneidabad²

2020

bta

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Mulberry (Morus alba L.) is an ornamental, medicinal, and fruit plant that belongs to the Moraceae family. One of the most important techniques used in plant biotechnology is tissue culture, which enables mass production of pathogen-free plants. Cotyledon has a high potential for shoot regeneration; however, to the best of our knowledge, there are no reports on plant regeneration from cotyledon explants of mulberry. Therefore, this study aimed to evaluate the potential of seedling-derived cotyledon segments to obtain shoot multiplication of mulberry. Various concentrations (0, 0.25, 0.5, 1, 2, 3, and 4 mg/l) of thidiazuron (TDZ) in combination with indole butyric acid (IBA) were used in a completely randomized design in three replications. The results showed that the highest percentage of regeneration frequency (96.67%) and the maximum number of shoots (4.43) were obtained on Murashige and Skoog (MS) medium supplemented with 0.25 mg/l TDZ and 0.025 mg/l IBA. In the rooting experiment, the maximum rooting percentage (83.33%) and the maximum number of roots per shoot (4.36) were obtained on MS medium containing 2 mg/l IBA. In vitro-raised plantlets were placed in pots and kept in room temperature for 30 days, and the plantlets showed more than 90% survival rate. On the basis of our results, the protocol described in this study has a high potential to be used in the micropropagation of this valuable plant.

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Modeling and Optimizing in vitro Sterilization of Chrysanthemum via Multilayer Perceptron-Non-dominated Sorting Genetic Algorithm-II (MLP-NSGAII)

Cited by 55 publications

References 58 publications

Advances of native and non-native Antarctic species to in vitro conservation: improvement of disinfection protocols

Advances of native and non-native Antarctic species to in vitro conservation: improvement of disinfection protocols

The application of artificial neural networks in modeling and predicting the effects of melatonin on morphological responses of citrus to drought stress

Effects of thidiazuron on in vitro shoot regeneration of Morus alba

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