Model studies of chlorophyll fluorescence reabsorption at chloroplast level under different exciting conditions

Abstract

A theoretical model is presented describing the distortion of chlorophyll fluorescence spectra of a chloroplast or a group of chloroplasts by the effect of fluorescence reabsorption. Model calculations using the experimental data show that the primary reabsorption effect occurs already within one chloroplast and the spectral distortion depends significantly on the excitation regime of the chloroplast. A theoretical dependence of the distortion function, defined as a change in the F(685)/F(735) fluorescence band ratio, on the mean chlorophyll concentration in a chloroplast is predicted for different light excitation regimes. The distortion of measured chlorophyll fluorescence spectra at 77 K of chloroplast suspension adsorbed on filter papers of two strongly different diffusive reflectivities and at different mean chlorophyll concentrations are discussed with the help of the presented theory.

Light quality, oxygenic photosynthesis and more

“…Going back to the data on ChlF, the measured signal is known to be affected by reabsorption of fluorescence (Agati et al 1993, Nauš et al 1994, Gitelson et al 1998, Romero et al 2018. This is because the major (685 nm) part of the ChlF emission spectrum overlaps with the major Chl absorption band (at 680 nm).…”

A Monte Carlo study of the chlorophyll fluorescence emission and its effect on the leaf spectral reflectance and transmittance under various conditions

“…Nauš et al 20 used a linear combination of three perpendicular modes of excitation in order to model the light environment in leaves. The model, applied for cubic representation of one chloroplast, qualitatively predicted distortion of the fluorescence emission by the fluorescence reabsorption even on the chloroplast level.…”

Optical Properties of Corals Distort Variable Chlorophyll Fluorescence Measurements

“…Such gradients can also affect measurements of the Chl a fluorescence kinetics (Sušila et al, 2004). Reabsorption of fluorescence emission can pose a challenge for Chl a fluorimetry in optically dense tissues (Naus et al, 1994;Bartošková et al, 1999). A method for correcting variable fluorescence measurements in optically dense algal media under constant optical geometries (e.g.…”