Model plants for studying the interaction between <i>Methylobacterium mesophilicum</i> and <i>Xylella fastidiosa</i>

Andreote, Fernando Dini; Lacava, Paulo Teixeira; Gai, Claudia S.; Araújo, Welington Luiz; Maccheroni, Walter; Overbeek, L.S. van; Elsas, Jan Dirk van; Azevedo, João Lúcio

doi:10.1139/w05-142

Cited by 65 publications

(73 citation statements)

References 18 publications

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“…Shifts in endophytic bacterial communi-ties are therefore expected to occur after bacterial inoculation. This is a phenomenon that has been observed after inoculation of Madagascar periwinkle (Catharanthus roseus) and Cleveland tobacco (Nicotiana clevelandii) plants with Methylobacterium mesophilicum (1).…”

mentioning

confidence: 92%

Endophytic Colonization of Potato ( Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, Pseudomonas putida Strain P9, and Its Effect on Associated Bacterial Communities

Andreote

Araújo

Azevedo

et al. 2009

Appl Environ Microbiol

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103

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Pseudomonas putida strain P9 is a novel competent endophyte from potato. P9 causes cultivar-dependent suppression of Phytophthora infestans. Colonization of the rhizoplane and endosphere of potato plants by P9 and its rifampin-resistant derivative P9R was studied. The purposes of this work were to follow the fate of P9 inside growing potato plants and to establish its effect on associated microbial communities. The effects of P9 and P9R inoculation were studied in two separate experiments. The roots of transplants of three different cultivars of potato were dipped in suspensions of P9 or P9R cells, and the plants were planted in soil. The fate of both strains was followed by examining colony growth and by performing PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Colonies of both strains were recovered from rhizoplane and endosphere samples of all three cultivars at two growth stages. A conspicuous band, representing P9 and P9R, was found in all Pseudomonas PCR-DGGE fingerprints for treated plants. The numbers of P9R CFU and the P9R-specific band intensities for the different replicate samples were positively correlated, as determined by linear regression analysis. The effects of plant growth stage, genotype, and the presence of P9R on associated microbial communities were examined by multivariate and unweighted-pair group method with arithmetic mean cluster analyses of PCR-DGGE fingerprints. The presence of strain P9R had an effect on bacterial groups identified as Pseudomonas azotoformans, Pseudomonas veronii, and Pseudomonas syringae. In conclusion, strain P9 is an avid colonizer of potato plants, competing with microbial populations indigenous to the potato phytosphere. Bacterization with a biocontrol agent has an important and previously unexplored effect on plant-associated communities.The colonization of plant tissue is of paramount importance for successful application of plant-growth-promoting bacteria. For instance, efficient root colonization was shown to be important for the control of Fusarium oxysporum in tomato by a phenazine-1-carboxamide-producing Pseudomonas chlororaphis strain (6). The migratory response of bacterial inoculants to compounds released by plant roots is often the first step required for establishment of the bacteria in the rhizosphere and rhizoplane (29,48,50). Following the initial colonization by introduced bacteria, these organisms may spread further to the aerial parts of the plant (35). The degree of root colonization by bacterial inoculants depends on, in addition to the mode of application (35), factors intrinsic to the organism used, like the presence of flagella (11) and/or the presence of particular outer membrane lipopolysaccharides (13). Such features may differ from strain to strain. Once attached to plant roots, the inoculant bacteria may evoke "protective" responses in the plants, enabling them to resist phytopathogen attack (4).Endophytic colonization, characterized by colonization of internal plant tissues concomitant with growth and systemic spread, can ...

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mentioning

confidence: 92%

Endophytic Colonization of Potato ( Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, Pseudomonas putida Strain P9, and Its Effect on Associated Bacterial Communities

Andreote

Araújo

Azevedo

et al. 2009

Appl Environ Microbiol

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103

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“…community and its interactions with X. fastidiosa [6,12]. The results of these studies found the pathogenic bacterium X. fastidiosa caused a shift in the indigenous endophytic community structure [13]. Genetically modified Enterobacter cloacae was able to colonize plants and to induce shifts in bacterial communities associated with citrus seedlings [14].…”

Section: Introductionmentioning

confidence: 99%

Endophytic population of Pantoea agglomerans in citrus plants and development of a cloning vector for endophytes

Andreote¹,

Rossetto²,

Souza³

et al. 2008

J. Basic Microbiol.

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Harmless bacteria inhabiting inner plant tissues are termed endophytes. Population fluctuations in the endophytic bacterium Pantoea agglomerans associated with two species of field cultured citrus plants were monitored over a two-year period. The results demonstrated that populations of P. agglomerans fluctuated in Citrus reticulata but not C. sinensis. A cryptic plasmid pPA3.0 (2.9 kb) was identified in 35 out of 44 endophytic isolates of P. agglomerans and was subsequently sequenced. The origins of replication were identified and nine out of 18 open reading frames (ORFs) revealed homology with described proteins. Notably, two ORFs were related to cellular transport systems and plasmid maintenance. Plasmid pPA3.0 was cloned and the gfp gene inserted to generate the pPAGFP vector. The vector was introduced into P. agglomerans isolates and revealed stability was dependent on the isolate genotype, ninety-percent stability values were reached after 60 hours of bacterial cultivation in most evaluated isolates. In order to definitively establish P. agglomerans as an endophyte, the non-transformed bacterium was reintroduced into in vitro cultivated seedlings and the density of inner tissue colonization in inoculated plants was estimated by bacterium re-isolation, while the tissue niches preferred by the bacterium were investigated by scanning electronic microscopy (SEM). Cells from P. agglomerans (strain ARB18) at similar densities were re-isolated from roots, stems and leaves and colonization of parenchyma and xylem tissues were observed. Data suggested that P. agglomerans is a ubiquitous citrus endophyte harboring cryptic plasmids. These characteristics suggest the potential to use the bacterium as a vehicle to introduce new genes in host plants via endophytic bacterial transformation.

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“…Com esta colonização sistêmica da planta, estas bactérias podem alterar as condições fisiológicas e morfológicas do hospedeiro, além de atuar sobre as populações de outros microrganismos presentes no interior da planta (ANDREOTE et al, 2004;ANDREOTE et al, 2006). Entre as enzimas produzidas por estes microrganismos, podem ser citadas amilases (STAMFORD et al, 2001), exolevanases (MENENDEZ et al, 2002), quitinases (PLEBAN et al, 1997), glucanases (MOY et al, 2002) e proteases (REDDY et al, 1996), as quais podem atuar em processos importantes na interação microrganismo-planta.…”

Section: )unclassified

“…estudos mostram que a produção de biofilme pode ser dependente da produção de moléculas de quorum sensing (DAVIES et al, 1998;NIETO-PEÑALVER et al, 2006), e que a formação de biofilme sobre a planta pode ser a primeira etapa para a colonização endofítica na interação Methylobacterium-planta (ANDREOTE et al, 2006), sugerindo a importância do entendimento destes processos envolvidos na interação bactéria-planta.…”

Section: Biofilmes Bacterianos E Comunicação Celular (Quorum Sensing)unclassified

Interação entre Methylobacterium extorquens e cana-de-açúcar (Saccharum sp.)

Silva¹

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AGRADECIMENTOSÀ Deus, por me guiar e me dar coragem para superar os momentos difíceis; À meus pais e meus irmãos por todo apoio, amor e compreenção em mais esta etapa da minha vida; Ao Dr. Welington Luiz de Araújo, pela orientação, apoio e amizade nesses dois anos de trabalho; À Profa. Dra. Aline Aparecida Pizzirani-Kleiner e prof. Dr. João Lúcio de Azevedo, pela amizade e receptividade; Ao Prof. Dr. Carlos Alberto Labate, pela disponibilização integral da excelente infra estrutura de seu laboratório, indispensáveis a realização deste trabalho; Ao Departamento de Genética da ESALQ/USP, funcionários e colegas, que foram importantes em algum passo para a realização deste trabalho;Aos coordenadores e professores do curso de pós graduação em Genética e Melhoramento de por RAPD ............................................................................................................31 parcial do 16S rDNA ...................................................................... ...........................................................................................................33 do extrato proteíco.............................................................................. ......................................................................................................................... em gel de poliacrilamida .........................................................................35 de imagem dos géis...................................................................................... extorquens na planta hospedeira .......................................................36 M. extorquens .......................................................................................36 M. extorquens ............................................... As plantas quando colonizadas produzem diversas enzimas de defesa que podem impedir o estabelecimento de microrganismos. Esta condição adversa na planta hospedeira gera uma resposta do microrganismo, a qual está associada à síntese de proteínas e outras moléculas que atuam na sua interação com a planta e alteram a comunidade microbiana associada. Todos os organismos respondem a essa condição, estabelecendo biofilmes, ou sintetizando um grupo de moléculas e proteínas que os protegem de danos e facilita a recuperação. Estas são chamadas proteínas de choque térmico (HSPs), as quais não foram ainda estudadas na interação Methylobacterium -planta. As bactérias do gênero Methylobacterium são metilotróficas facultativas da classe Alfa-proteobactéria, encontradas em relações epifíticas e endofíticas com diferentes espécies vegetais. Assim, o presente trabalho teve como objetivos avaliar o efeito da deficiência da produção de biofilme e de Acil-Homoserina-Lactonas (AHLs), e do estresse térmico de M. extorquens na colonização da planta hospedeira (Saccharum sp.). Para isso, foram usadas linhagens defectivas para produção de biofilme e AHLs, juntamente com a linhagem selvagem submetida ou não ao estresse térmico. Os resultados obtidos mostram a complexidade dos mecanismos envo...

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Model plants for studying the interaction between Methylobacterium mesophilicum and Xylella fastidiosa

Cited by 65 publications

References 18 publications

Endophytic Colonization of Potato ( Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, Pseudomonas putida Strain P9, and Its Effect on Associated Bacterial Communities

Endophytic Colonization of Potato ( Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, Pseudomonas putida Strain P9, and Its Effect on Associated Bacterial Communities

Endophytic population of Pantoea agglomerans in citrus plants and development of a cloning vector for endophytes

Interação entre Methylobacterium extorquens e cana-de-açúcar (Saccharum sp.)

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