Model of separated form factors for unilamellar vesicles

Киселев, М. А.; Lesieur, P.; Kisselev, A.M.; Lombardo, Domenico; Аксенов, В. Л.

doi:10.1007/s003390201837

Cited by 51 publications

(50 citation statements)

References 8 publications

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“…The analysis of the SANS curves has been performed using two methods, namely the 'model of separated form factors' (MSFF) [23], and the Kratky-Porod analysis of the Guinier approximation (GA) [24][25][26].…”

Section: Small-angle Neutron Scatteringmentioning

confidence: 99%

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Influence of cholesterol on the structure of stratum corneum lipid model membrane

Zbytovská

Киселев

Funari³

et al. 2008

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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a b s t r a c tThis study describes the influence of cholesterol on a model membrane consisting of four stratum corneum (SC) lipids in three different states at 32 and 85 • C. Using small-angle X-ray diffraction on multilamellar vesicles, the lamellar repeat distance, D, was determined. Small-angle neutron scattering on unilamellar vesicles was used to calculate the membrane thickness, average area of the membrane surface per molecule, average volume per molecule in the membrane, and membrane density. After the sample preparation, the membranes show one lamellar phase. Over 10 days, the systems separate into two lamellar phases and crystalline cholesterol. On heating the samples to 85 • C, the domains merge into one phase, the D-value of which increases slightly with increasing cholesterol concentration. On cooling the samples back to 32 • C, only one phase is observable again. The increasing cholesterol concentration in the membrane causes a decrease in the D-value, membrane thickness, and membrane density. Simultaneously, the area of the membrane surface and membrane volume per molecule increase. In conclusion, cholesterol fluidises the SC lipid membranes in the state below the main phase transition and condenses above it. This effect can have an important impact on the pathologic skin states such as the recessive X-linked ichthyosis.

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Section: Small-angle Neutron Scatteringmentioning

confidence: 99%

“…(2) as R = /q R min , where q R min is the first minimum in the form factor of infinitely thin spheres after averaging the population of polydisperse vesicles [23,28].…”

Section: Small-angle Neutron Scatteringmentioning

confidence: 99%

Influence of cholesterol on the structure of stratum corneum lipid model membrane

Zbytovská

Киселев

Funari³

et al. 2008

Colloids and Surfaces A: Physicochemical and Engineering Aspect

Self Cite

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show abstract

“…The analysis of the SANS curves has been performed using two methods, namely the "model of separated form factors" (Kiselev et al, 2002), and the Kratky-Porod analysis of the Guinier approximation (Knoll et al, 1981;Feigin and Svergun, 1987;Balgavy et al, 2001).…”

Section: Small Angle Neutron Scatteringmentioning

confidence: 99%

Influence of phytosphingosine-type ceramides on the structure of DMPC membrane

Zbytovská

Киселев

Funari

et al. 2005

Chemistry and Physics of Lipids

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“…SANS is a well-established technique for the investigation of lipid vesicles 13 and the change of the parameters describing their structure in different conditions. [14][15][16][17] We have applied the recently proposed separated form factor (SFF) method 18 to extruded unilamellar vesicles, and we have extracted the parameters characterizing the vesicle size, size distribution, and vesicle shell (i.e., lipid bilayer) by using different models to describe the bilayer profiles. The effect of Aβ on these parameters has been investigated as a function of pH and of Aβ concentration.…”

Section: Introductionmentioning

confidence: 99%