Model for type 2 diabetes exhibits changes in vascular function and structure due to vascular oxidative stress and inflammation

Azemi, Ahmad Khusairi; Mokhtar, Siti Safiah; Hou, Low Jen; Sharif, Sharifah Emilia Tuan; Rasool, Aida Hanum Ghulam

doi:10.1080/10520295.2020.1823480

Cited by 10 publications

(12 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increased aortic oxidative stress plays a significant role in the development of atherosclerosis (Azemi, Mokhtar, Low, et al. 2020 ). This study also demonstrated that CNME treatment reduced MDA levels, as well as increased SOD activity in the thoracic aortas of diabetic rats.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Clinacanthus nutans attenuates atherosclerosis progression in rats with type 2 diabetes by reducing vascular oxidative stress and inflammation

Azemi

Mokhtar

Sharif

et al. 2021

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

Context Atherosclerosis predisposes individuals to adverse cardiovascular events. Clinacanthus nutans L. (Acanthaceae) is a traditional remedy used for diabetes and inflammatory conditions. Objectives To investigate the anti-atherosclerotic activity of a C. nutans leaf methanol extract (CNME) in a type 2 diabetic (T2D) rat model induced by a high-fat diet (HFD) and low-dose streptozotocin. Materials and methods Sixty male Sprague-Dawley rats were divided into five groups: non-diabetic fed a standard diet (C), C + CNME (500 mg/kg, orally), diabetic fed an HFD (DM), DM + CNME (500 mg/kg), and DM + Metformin (DM + Met; 300 mg/kg). Treatment with oral CNME and metformin was administered for 4 weeks. Fasting blood glucose (FBG), serum lipid profile, atherogenic index (AI), aortic tissue superoxide dismutase levels (SOD), malondialdehyde (MDA), and tumour necrosis factor-alpha (TNF-α) were measured. The rats’ aortas were stained for histological analysis and intima-media thickness (IMT), a marker of subclinical atherosclerosis. Results The CNME-treated diabetic rats had reduced serum total cholesterol (43.74%; p = 0.0031), triglycerides (80.91%; p = 0.0003), low-density lipoprotein cholesterol (56.64%; p = 0.0008), AI (51.32%; p < 0.0001), MDA (60.74%; p = 0.0026), TNF-α (61.78%; p = 0.0002), and IMT (39.35%; p < 0.0001) compared to untreated diabetic rats. SOD level, however, increased (53.36%; p = 0.0326). These CNME effects were comparable to those in the metformin-treated diabetic rats. Conclusions C. nutans possesses anti-atherosclerotic properties, which may be due to reductions in vascular tissue oxidative stress, inflammation, and serum AI. Continued studies on atherosclerotic animal models are suggested.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The histology study was carried out according to the method described by Azemi, Mokhtar, Low, et al. ( 2020 ). The thoracic aortas were preserved in 10% formalin.…”

Section: Methodsmentioning

confidence: 99%

Clinacanthus nutans attenuates atherosclerosis progression in rats with type 2 diabetes by reducing vascular oxidative stress and inflammation

Azemi

Mokhtar

Sharif

et al. 2021

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has been reported that HFD-induced hyperlipidemia results in a significant increase in serum TC, TG, LDL-C, and AI [ 23 , 24 , 41 ]. The present study demonstrated that TRF for six weeks reduced the levels of TC, TG, LDL-C, and AI in obese rats.…”

Section: Discussionmentioning

confidence: 99%

“…A high-fat diet (HFD) was prepared according to a method described previously [ 23 ]. The HFD was prepared with a mixture of 50% ( w / w ) standard diet (Gold Coin Feedmils, Port Klang, Malaysia), 38% ( w / w ) ghee, 8% ( w / w ) full-cream milk, and 4% ( w / w ) white sugar.…”

Section: Methodsmentioning

confidence: 99%

“…For non-TRF groups (NR and OR groups), food was provided ad libitum , available to be eaten for 24 h. Diets were given in the same standardized amount daily for all groups, however, the groups undergoing TRF could only consume them within an 8 h duration per day. At the end of the twelfth week, the rats were euthanized by intraperitoneal injection of a combination of ketamine (300 mg/kg) and xylazine (30 mg/kg) [ 23 ]. A blood sample from the renal artery was collected from each rat and placed in standard vacutainer tubes.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Time-Restricted Feeding Improved Vascular Endothelial Function in a High-Fat Diet-Induced Obesity Rat Model

Azemi

Siti-Sarah

Mokhtar

et al. 2022

Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

Obesity, where there is enhancement of stored body fat in adipose tissues, is associated with cardiovascular complications that are mainly related to atherosclerosis. Time-restricted feeding (TRF) is a form of restricted eating aimed at reducing weight in obese subjects. The present study aims to investigate changes in vascular endothelial function, endothelial nitric oxide synthase (eNOS), and protein kinase B (Akt) protein expressions with TRF in obese and normal rats. Male Sprague Dawley rats were divided into two normal and three obese groups; obesity was induced in the obese groups by feeding with a high-fat diet (HFD) for six weeks. After six weeks, rats were equally divided into five groups (n = 7 per group): Normal group (NR) which continued on a standard diet for six more weeks, normal group switched to TRF with a standard diet for six weeks (NR + TRFSD), obese group (OR) which continued on HFD for six more weeks, obese group switched to TRF of HFD (OR + TRFHFD), and obese group switched to TRF of a standard diet (OR + TRFSD). TRF was practiced for six weeks, after which the rats were sacrificed. Aortic endothelium-dependent and endothelium-independent relaxations and contractions were assessed using the organ bath. Aortic eNOS and Akt protein expressions were determined using immunoblotting. Fasting blood glucose, body weight, body mass index (BMI), serum lipid profile, Lee’s index, serum insulin levels, and sensitivity (HOMA-IR) were also measured. Endothelium-dependent relaxation was significantly impaired, while endothelium-dependent contraction increased in obese rats compared to that in normal rats. Both obese groups which underwent TRF with a HFD and standard diet improved their impairments in endothelium-dependent relaxation and reduced endothelium-dependent contraction; these were associated with increased expressions of aortic eNOS and Akt protein. Both obese groups with TRF reduced body weight, BMI, Lee’s index, total cholesterol, triglycerides, low-density lipoprotein cholesterol, and improved insulin sensitivity. TRF improved endothelium-dependent relaxation and reduced endothelium-dependent contraction, thus attenuating endothelial dysfunction in obese rats. These were associated with increased aortic eNOS and Akt protein expressions.

show abstract

Cardiovascular disease in diabetes, beyond glucose

2021

View full text Add to dashboard Cite

Model for type 2 diabetes exhibits changes in vascular function and structure due to vascular oxidative stress and inflammation

Cited by 10 publications

References 37 publications

Clinacanthus nutans attenuates atherosclerosis progression in rats with type 2 diabetes by reducing vascular oxidative stress and inflammation

Clinacanthus nutans attenuates atherosclerosis progression in rats with type 2 diabetes by reducing vascular oxidative stress and inflammation

Time-Restricted Feeding Improved Vascular Endothelial Function in a High-Fat Diet-Induced Obesity Rat Model

Cardiovascular disease in diabetes, beyond glucose

Contact Info

Product

Resources

About