Model for human skin reconstructed in vitro composed of associated dermis and epidermis

Souto, Luís Ricardo Martinhão; Rehder, Jussara; Vassallo, José; Cintra, Maria Letícia; Kraemer, Maria Helena Stangler; Puzzi, Maria Beatriz

doi:10.1590/s1516-31802006000200005

Cited by 39 publications

(36 citation statements)

References 35 publications

(36 reference statements)

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“…10 Since then, different models of reconstructed skin were developed, studied, and commercialized. 1,3,5,11,12 These models were characterized and compared by several techniques to natural skin, excised human skin, and animal model skin (rat and pig). 13,14 Among the techniques actually used to study the skin, Raman spectroscopy presents the advantages of being nondestructive, it also allows a real-time structural and molecular analysis, without requiring any sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

et al. 2007

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Human skin is directly exposed to different exogenous agents. Many research works have studied the diffusion, interactions, absorption mechanisms, and/or toxicity of these agents toward different cutaneous structures. With the use of living animals for such tests being more and more rejected; and the number of human volunteers being limited; different types of skin models are used. In the last few years, reconstructed epidermis from cell cultures has been frequently employed, and recent changes in the European chemical policy have approved and encouraged the use of these reconstructed models for skin‐related research works and assessments. Among the techniques used actually to study the skin, Raman microspectroscopy is a rising and powerful nondestructive technique that detects characteristic molecular vibrations. In this study, we created a spectral database to index the vibration peaks and bands of a well‐known reconstructed epidermis model, the Episkin®. The comparison with a native epidermis signal enabled us to put in evidence several spectral differences associated with molecular and structural differences between the skin and the reconstructed model, both maintained in living conditions. In addition to that, we have showed the feasibility of tracking the penetration of a pharmaceutical molecule through the Episkin model. © 2007 Wiley Periodicals, Inc. Biopolymers 87: 261–274, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

et al. 2007

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“…6 Weigert-Van Gieson staining showed a small number of thin elastic fibers in the dermal equivalent. The PAS reaction delineated the basement membrane as a continuous thin layer between the epidermis and dermis.…”

Section: Resultsmentioning

confidence: 95%

“…The technique used for obtaining reconstructed human skin in vitro was the one described by Souto et al 6 and it is summarized here. After obtaining skin samples from patients who had undergone mammoplasty or abdominoplasty, cell cultures were performed in specific growth media for each cell type: A. Culturing of epidermal epithelial cells in a culture medium for keratinocytes (Gibco BRL, Grand Island, New York, United States, catalog number 10785-012); B. Culturing of melanocytes in a specific culture medium (MCDB 153; Sigma Chemical Co., St. Louis, Missouri, United States, code M-7403); C. Culturing of fibroblasts in M199 culture medium (Gibco, catalog number 31100-035).…”

Section: Methodsmentioning

confidence: 99%

“…[1][2][3][4][5][6] These models represent an alternative system for testing pharmacological products in the place of laboratory animals. 7 The European Union, through its "Sixth Amendment to the European Community Cosmetic Directive", has been aiming towards banning the use of animals in tests for certain types of cosmetic ingredients, combinations of ingredients and final products since January 1, 1998.…”

Section: Introductionmentioning

confidence: 99%

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Immunoarchitectural characterization of a human skin model reconstructed in vitro

et al. 2009

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CONTEXT AND OBJECTIVE: Over the last few years, different models for human skin equivalent reconstructed in vitro (HSERIV) have been reported for clinical usage and applications in research for the pharmaceutical industry. Before release for routine use as human skin replacements, HSERIV models need to be tested regarding their similarity with in vivo skin, using morphological (architectural) and immunohistochemical (functional) analyses. A model for HSERIV has been developed in our hospital, and our aim here was to further characterize its immunoarchitectural features by comparing them with human skin, before it can be tested for clinical use, e.g. for severe burns or wounds, whenever ancillary methods are not indicated. DESIGN AND SETTING:Experimental laboratory study, in the Skin Cell

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“…Besides, EOM are less exposed to mutation agents compared with other skin epithelial tissues (Muotri et al 2004;Souto et al 2006). EOM DNA is used to diagnose several diseases (Aidar and Line 2007), such as demonstrated to determine mutations in the PraderWilli syndrome (PWS) (Munce et al 2008), verifying the increasing risk for gonadoblastoma in Turner syndrome patients (Bianco et al 2006), or analyzing segregation in DNA 3243 A[G mutation in mitochondrial diseases in children and their mothers (Uusimaa et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Epithelial cells from oral mucosa: How to cultivate them?

et al. 2016

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Epithelial cells from oral mucosa (EOM) are responsible for important functions, like the primary protection of oral mucosa against external aggressions building a mechanical barrier against microorganisms, mechanical damage, toxic material, thermal regulation and secretion of different classes of inflammatory mediators. EOM could be an interesting tool for cellular and molecular biology research. Usually, EOM are collected by a painful and invasive process. In this study, we propose an alternative method to cultivate EOM collected by non-invasive scraping method of oral mucosa. Papanicolaou staining showed mainly two kinds of epithelial cell population after EOM scraping.As result of the five culture methods tested here, our results revealed that the EOM were successfully cultured on a murine feeder layer. In addition, EOM could be frozen and thawed, without morphology changes and loss of viability. Our findings suggest that EOM can be considered as a good cell source for many purposes, such as genetic studies, diagnosis and cell therapy.

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Model for human skin reconstructed in vitro composed of associated dermis and epidermis

Abstract: It is possible to obtain completely differentiated human skin composed of associated dermis and epidermis, reconstructed in vitro, from injection of human fibroblasts into bovine collagen type I matrix and culturing of human keratinocytes and melanocytes on this matrix.

Cited by 39 publications

References 35 publications

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

Immunoarchitectural characterization of a human skin model reconstructed in vitro

Epithelial cells from oral mucosa: How to cultivate them?

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