Mobilisation of mesenchymal cells into blood in response to skeletal muscle injury

Ramı́rez, Manuel; Lucı́a, Alejandro; Gómez‐Gallego, Félix; Esteve-Lanao, Jonathan; Pérez‐Martínez, Antonio; Foster, Carl; Andreu, Antoni L.; Martin, Matthew A.; Madero, Luís; Arenas, Joaquı́n; García‐Castro, Javier

doi:10.1136/bjsm.2006.028639

Cited by 57 publications

(42 citation statements)

References 17 publications

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“…Blood samples were collected from the anti-cubital vein 16. In order to remove the potentially confounding effect of increased MC mobilisation into the blood in response to skeletal muscle injury,16 serum total creatine kinase (CK) activity (a marker for skeletal muscle injury)19 was measured in all samples with an automated analyser (Hitachi 911, Boehringer Mannheim, Mannheim, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…We recently showed increased mobilisation of MCs into the blood of healthy individuals, peaking 2 h after an acute bout of prolonged exercise which induced skeletal muscle damage 16. We were interested in whether intense exercise (i.e., near the ischaemic threshold) might stimulate MC migration into the blood of patients with stable CAD as has previously been shown with EPCs 5…”

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confidence: 97%

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Mobilisation of mesenchymal cells in cardiac patients: is intense exercise necessary?: Figure 1

Lucı́a

Rosa²,

Silván³

et al. 2008

Br J Sports Med

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Circulating mesenchymal cells (cMCs) have a potential for regenerating damaged tissue, e.g., ischaemic myocardium. In patients (age range: 53-76 years) with stable coronary artery disease cMCs were determined before and after dynamic exercise of moderate (< respiratory compensation threshold (RCT)) (n = 9 patients) or high intensity (>RCT) (n = 11). Only high-intensity exercise (i.e., provoking signs of myocardial ischaemia in 3 patients and ventricular extrasystoles in another) induced a significant increase in cMCs (p = 0.009). These results support the hypothesis that intense exercise (near or at the point of myocardial ischaemia) is a potent stimulus for MC mobilisation.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 97%

Mobilisation of mesenchymal cells in cardiac patients: is intense exercise necessary?: Figure 1

Lucı́a

Rosa²,

Silván³

et al. 2008

Br J Sports Med

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“…There are numerous studies suggesting that MSCs are stimulated to leave their niche, released into circulation and recruited into damaged tissues under injury conditions [16,17,33]. To rule out lesion-related stem cell mobilization, we therefore used wild-type rats instead of a disease model in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, it has become apparent that a small number of MSCs can be mobilized into the PB in response to injury signals [16,17,33] or following appropriate stimuli [18,19]. Further evidence suggests that combinations of growth factors with the CXCR4 antagonist AMD3100 effectively induces release of MSCs into the peripheral circulation [34,35].…”

Section: Discussionmentioning

confidence: 99%

Electro-Acupuncture Promotes Endogenous Multipotential Mesenchymal Stem Cell Mobilization into the Peripheral Blood

Liu

et al. 2016

Cell Physiol Biochem

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Background/Aims: Mobilization of endogenous stem cells is an appealing strategy for cell therapy However, there is little evidence for reproducible, effective methods of mesenchymal stem cell (MSC) mobilization. In the present study, we investigated the mobilizing effect of electro-acupuncture (EA) on endogenous MSCs. Methods: Normal adult rats were randomly divided into six groups, namely, EA for 14 days (EA14d), sham EA14d, EA21d, sham EA21d and matched control groups. MSC mobilization efficiency was determined by colony-forming unit fibroblast (CFU-F) assays. Mobilized peripheral blood (PB)-derived MSCs were identified by immunophenotype and multi-lineage differentiation potential. Results: CFU-F frequency was significantly increased in the PB of EA14d rats compared with the sham EA and control groups. Moreover, the number of CFU-Fs was increased further in the EA21d group. MSCs derived from EA-mobilized PB were positive for CD90 and CD44, but negative for CD45. Additionally, these cells could differentiate into adipocytes, osteoblasts, chondrocytes and neural-like cells in vitro. Finally, stromal cell-derived factor-1α (SDF-1α) was increased in the PB of rats subjected to EA, and the migration of MSCs was improved in response to SDF-1α. Conclusions: MSCs with multi-lineage differentiation potential can be mobilized by EA. Our data provide a promising strategy for MSC mobilization.

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“…On the other hand, numerous studies indicate that SMSP are derived from bone marrow. It has been shown, that both acute and chronic injury of muscle result in mobilization of mesenchymal stem cells from bone morrow to the peripheral blood [47]. Circulating bone marrow-derived cells are able to settle in skeletal muscle and contribute to their regeneration [18,42,[48][49][50].…”

Section: Origin Of Myogenic Stem Cellsmentioning

confidence: 99%

Myogenic stem cells.

Burdzińska

Gala²,

Pączek³

2009

Folia Histochem Cytobiol.

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Both skeletal muscle and bone marrow tissue contain myogenic stem cells. The population residing in muscles is heterogenic. Predominant in number are "typical" satellite cells -muscle progenitors migrating from somites during embryonic life. Another population is group of multipotent muscle stem cells which, at least in part, are derived from bone marrow. These cells are tracked by gradient of growth factors releasing from muscle during injury or exercise. Recruited bone marrow-derived cells gradually change their phenotype becoming muscle stem cells and eventually can attain satellite cell position and express Pax7 protein. Mesenchymal stem cells (MSC) isolated directly from bone marrow also display myogenic potential, although methods of induction of myogenic differentiation in vitro have not been optimized yet. Concerning efforts of exploiting myogenic stem cells in cell-mediated therapies it is important to understand the cause of impaired regenerative potential of aged muscle. Up to now, most of research data suggest that majority of age related changes in skeletal muscles are reversible, thus depending on extrinsic factors. However, irreversible intrinsic features of muscle stem cells are also taken into consideration.

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Mobilisation of mesenchymal cells into blood in response to skeletal muscle injury

Cited by 57 publications

References 17 publications

Mobilisation of mesenchymal cells in cardiac patients: is intense exercise necessary?: Figure 1

Mobilisation of mesenchymal cells in cardiac patients: is intense exercise necessary?: Figure 1

Electro-Acupuncture Promotes Endogenous Multipotential Mesenchymal Stem Cell Mobilization into the Peripheral Blood

Myogenic stem cells.

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