Mobile Phases and Their Properties

Moldoveanu, Serban C.; David, Víctor

doi:10.1016/b978-0-12-385013-3.00007-0

Cited by 21 publications

(17 citation statements)

References 93 publications

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“…The procedure to build the phantom was described in detail in Bianchini et al 22 Briefly, a pelvic‐shaped container (NEMA IEC Body Phantom Set; Spectrum Corporation, Durham, NC) was used as the main phantom compartment, filled with a solution of 0.4mM MnCl 2 to reproduce the relaxation time T 2 of the muscle (40 to 65 ms at 1.5T 22 ) surrounding pelvic lesions. To study the repeatability on scanners B and C and the reproducibility between these scanners, the filling solution was replaced with oil (Spectrasyn 4 phantom oil; Philips Healthcare; T 1 = 230 ms and T 2 = 194 ms at 3T), which has a lower dielectric constant than water, 23,24 allowing to avoid dielectric artifacts intensified at higher fields. Four inserts (Figure 2A), created by mixing polystyrene spheres of different diameters (1 to 8 mm) and 0.1% water solution of agar were embedded in the solution to provide a MR average signal and texture consistent with a set of representative pelvic tumors.…”

Section: Methodsmentioning

confidence: 99%

A multicenter study on radiomic features from T₂‐weighted images of a customized MR pelvic phantom setting the basis for robust radiomic models in clinics

Bianchini

Santinha

Loução³

et al. 2020

Magnetic Resonance in Med

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To investigate the repeatability and reproducibility of radiomic features extracted from MR images and provide a workflow to identify robust features. Methods: T 2-weighted images of a pelvic phantom were acquired on three scanners of two manufacturers and two magnetic field strengths. The repeatability and reproducibility of features were assessed by the intraclass correlation coefficient and the concordance correlation coefficient, respectively, and by the within-subject coefficient of variation, considering repeated acquisitions with and without phantom repositioning, and with different scanner and acquisition parameters. The features showing intraclass correlation coefficient or concordance correlation coefficient >0.9 were selected, and their dependence on shape information (Spearman's ρ > 0.8) analyzed. They were classified for their ability to distinguish textures, after shuffling voxel intensities of images. Results: From 944 two-dimensional features, 79.9% to 96.4% showed excellent repeatability in fixed position across all scanners. A much lower range (11.2% to 85.4%) was obtained after phantom repositioning. Three-dimensional extraction did not improve repeatability performance. Excellent reproducibility between scanners was observed in 4.6% to 15.6% of the features, at fixed imaging parameters. In addition, 82.4% to 94.9% of the features showed excellent agreement when extracted from images acquired with echo times 5 ms apart, but decreased with increasing 1714 | BIANCHINI et Al.

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Section: Methodsmentioning

confidence: 99%

A multicenter study on radiomic features from T₂‐weighted images of a customized MR pelvic phantom setting the basis for robust radiomic models in clinics

Bianchini

Santinha

Loução³

et al. 2020

Magnetic Resonance in Med

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“…According to these previous findings, we then carried out a study to investigate the cytotoxicity of these three medicinal plants in a different polarity of organic solvents. The selection of five different solvents used in this present study was referred to their polarity which correlated with the dielectric constant [15] . The polarity of solvent from highest to lowest are methanol (32.7), ethanol (24.6), dichloromethane (9.08), ethyl acetate (6.02) and hexane (1.9), respectively [16] .…”

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confidence: 99%

Dichloromethane Crude Extract of Gymnanthemum extensum Combined with Low Piperine Fractional Piper nigrum Extract Induces Apoptosis on Human Breast Cancer Cells

Faisal¹,

Maungchanburee²,

Dokduang³

et al. 2021

ijps

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Faisal et al.: G. Extensum Combined with P. Nigrum Induces Apoptosis This study aims to investigate the cytotoxicity of Andrographis paniculata, Ziziphus spina-christi and Gymnanthemum extensum crude extracts and their combination with low piperine fractional Piper nigrum extract. All plants were extracted with water and five organic solvents (methanol, ethanol, dichloromethane, ethyl acetate and hexane). Cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay on three cancer types (breast, colorectal and ovarian cancers) and two non-cancerous cells. The combination among extracts with low piperine fractional Piper nigrum extract was separately conducted in several tests including cytotoxicity, apoptosis and multi caspase activity. We found that, the dichloromethane crude extract of Andrographis paniculata, Ziziphus spinachristi and Gymnanthemum extensum exhibited the strongest cytotoxicity on colorectal cancer cells SW-620 (7.49±0.04 µg. ml -1 ), breast cancer cells Michigan Cancer Foundation-7 (13.35±0.30 µg. ml -1 ) and ovarian cancer cells A2780 (15.58±1.81 µg. ml -1 ), respectively. Gas chromatography mass spectrometry study of dichloromethane crude extract of Andrographis paniculata, Ziziphus spina-christi and Gymnanthemum extensum identified major compounds including 1-heptatriacotanol (60.29 %) and palmitic acid (26.92 % for dichloromethane crude extract of Ziziphus spina-christi and 21.40 % for dichloromethane crude extract of Gymnanthemum extensum), respectively. The combination of dichloromethane crude extract of Ziziphus spina-christi and low piperine fractional Piper nigrum extract at ratio IC 50 :0.5IC 50 showed a moderate synergistic effect on Michigan Cancer Foundation-7 cells. Interestingly, the mixture of dichloromethane crude extract of Gymnanthemum extensum with low piperine fractional Piper nigrum extract at ratio IC 50 :IC 50 , 0.5 IC 50 :IC 50 and IC 50 :0.5 IC 50 exhibited a synergistic effect on Michigan Cancer Foundation-7 cells. Moreover, combination of dichloromethane crude extract of Gymnanthemum extensum with low piperine fractional Piper nigrum extract induced the apoptosis and multi caspase activity in a time dependent manner. In conclusion, dichloromethane crude extract of Andrographis paniculata, Ziziphus spina-christi and Gymnanthemum extensum displayed potent anticancer activities and the combination of dichloromethane crude extract of Gymnanthemum extensum with low piperine fractional Piper nigrum extract can be a promising regimen for an alternative cancer treatment.

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“…in saliva and lactate in blood, respectively [35] α-Amylase is 50 kDa enzyme that has 3 main building domains (Fig. 2.3.2) [37]. The active site of α-amylase in located in the largest unit (domain A) and comprises of two aspartate residues and one glutamate residue.…”

Section: Biomarkers Of Overtraining In Salivamentioning

confidence: 99%

“…The amount of cortisol that binds directly to the cavities of MIP@paper can be explored by washing with methanol and AcA and compared with an identical set of experiments undertaken on non-imprinted polymer NIP@paper and nonmodified paper, respectively. In order to estimate the type of binding involved in retention of cortisol on the polymer surface, washing with methanol was chosen as it has lower dielectric constant than water (33 vs 80) and thus, facilitates disruption of van der Waals forces [37], and with acetic acid that breaks existing hydrogen bonds by changing the protonation state of polymer [38].…”

Section: Specificity Of Synthesized Mip@papermentioning

confidence: 99%

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Biomarker assay systems for onsite monitoring of fatigue

Tsyrulneva¹

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Overtraining is one of the most underestimated physiological states of athletes. Being recognized as a serious complication for high performance, overtraining is diagnosed based only on verbal complaint of a sportsman, making it highly subjective, or on exhibiting underperformance. Besides, numerous diseases and psychological disorders have the same symptoms. No specific biomarkers have been proposed for detection of overtraining, neither in blood or urine, nor in saliva. Various physiological, behavioral and biochemical markers are constantly examined. Recently, metabolic and hormonal biomarkers demonstrated their potential in assessing overtraining. The variations in concentration levels of salivary cortisol, testosterone and α-amylase are currently being used to evaluate the state of a sportsman. However, these changes can be caused by a variety of other disorders that greatly complicates the work of sport doctors. Presumably, constant monitoring of biomarkers' response to the influence of external and internal factors can provide tools for timely detection of early signs of overtraining development. In this work, saliva has been chosen as a biological fluid that can be tested onsite, being non-invasive and rapidly accessible object.Recently, the ratio of two salivary heptapeptides was reported to be indicative of overtraining condition. However, saliva as a complex matrix needs to be cleaned up in order to remove high weight proteins, debris and bacteria that otherwise would interfere with the detection of peptides. Few different extraction protocols were explored with the aim to extract the peptides from saliva. High Performance Liquid Chromatography with UV detection (HPLC/UV) and Matrix-Assisted Laser Desorption Ionization-Time of Flight Spectroscopy (MALDI/ToF) were used to identify peptides in extract. Quartz Crystal Microbalance (QCM) platform was developed with the aim to confirm the presence of peptides in salivary extract. A series of antibodies raised against each of peptide were tested in order to distinguish the one with the highest affinity towards a peptide. The surface chemistry was optimized to achieve the best binding of antibody to an immobilized peptide.

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Mobile Phases and Their Properties

Cited by 21 publications

References 93 publications

A multicenter study on radiomic features from T₂‐weighted images of a customized MR pelvic phantom setting the basis for robust radiomic models in clinics

A multicenter study on radiomic features from T₂‐weighted images of a customized MR pelvic phantom setting the basis for robust radiomic models in clinics

Dichloromethane Crude Extract of Gymnanthemum extensum Combined with Low Piperine Fractional Piper nigrum Extract Induces Apoptosis on Human Breast Cancer Cells

Biomarker assay systems for onsite monitoring of fatigue

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Mobile Phases and Their Properties

Cited by 21 publications

References 93 publications

A multicenter study on radiomic features from T2‐weighted images of a customized MR pelvic phantom setting the basis for robust radiomic models in clinics

A multicenter study on radiomic features from T2‐weighted images of a customized MR pelvic phantom setting the basis for robust radiomic models in clinics

Dichloromethane Crude Extract of Gymnanthemum extensum Combined with Low Piperine Fractional Piper nigrum Extract Induces Apoptosis on Human Breast Cancer Cells

Biomarker assay systems for onsite monitoring of fatigue

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Product

Resources

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A multicenter study on radiomic features from T₂‐weighted images of a customized MR pelvic phantom setting the basis for robust radiomic models in clinics

A multicenter study on radiomic features from T₂‐weighted images of a customized MR pelvic phantom setting the basis for robust radiomic models in clinics