Mobile FT mRNA contributes to the systemic florigen signalling in floral induction

Li, Chunyang; Gu, Muxin; Shi, Nongnong; Zhang, Hang; Yang, Xin; Osman, T. A. M.; Liu, Yule; Wang, Huizhong; Vatish, Manu; Jackson, Stephen D.; Hong, Yiguo

doi:10.1038/srep00073

Cited by 88 publications

(77 citation statements)

References 39 publications

(64 reference statements)

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“…4). This implies that neither PVX virus nor virally expressed AtFT mRNA is seed transmissible despite the fact that AtFT RNA is able to facilitate the entry of PVX to the apical shoot meristem (Li et al, 2011) and that PVX/AtFT infection cannot induce transgenerational epiflowering in progeny plants.…”

Section: Discussionmentioning

confidence: 99%

“…We previously showed that PVX-based expression of AtFT induces flowering in tobacco (Li et al, 2009(Li et al, , 2011. However, as the expression of the plant's own endogenous FT gene(s) is still possible, the induction of flowering could either be a direct effect of the virally expressed FT gene or caused by an indirect induction of the expression of the endogenous FT gene.…”

Section: Pvx-based Vifmentioning

confidence: 99%

“…FT protein or mRNA introduced by PVX promotes flowering in short-day (SD) N. tabacum MD Mammoth (MM) under noninductive (i.e. nonflowering) long-day (LD) conditions (Li et al, 2009(Li et al, , 2011. These findings open up possibilities for further development and application of the virus-induced flowering (VIF) assay in plants (McGarry et al, 2017).…”

mentioning

confidence: 99%

“…Moreover, in tomato rin and Cnr mutants, viral expression of the MADS-or SBP-box transcription factor LeMADS-RIN or LeSPL-CNR leads to virus-induced gene complementation and causes nonripening mutant fruits to ripen (Zhou et al, 2012;Kong et al, 2013). Another example is the use of virus technology to study the roles of proteins and RNAs in the flowering process (Li et al, 2011;McGarry et al, 2017).…”

mentioning

confidence: 99%

“…More recently, virus-induced genome editing has been employed to introduce mutations to specific genes and produce null mutants in plants (Baltes et al, 2014;Ali et al, 2015;Yin et al, 2015). Apart from these gene knockdown or knockout techniques, virus expression vectors have become a valuable tool to examine the mobility of cellular RNAs and the role of mobile RNA signals in flowering (Li et al, 2009(Li et al, , 2011, potato tuberization (Cho et al, 2015), and RNA silencing (Ryabov et al, 2004;Zhou et al, 2008;Qin et al, 2012).…”

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confidence: 99%

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A Virus-Induced Assay for Functional Dissection and Analysis of Monocot and Dicot Flowering Time Genes

Cheng

Chen²,

Shen³

et al. 2017

Plant Physiol.

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Virus-induced flowering (VIF) uses virus vectors to express Flowering Locus T (FT) to induce flowering in plants. This approach has recently attracted wide interest for its practical applications in accelerating breeding in crops and woody fruit trees. However, the insight into VIF and its potential as a powerful tool for dissecting florigenic proteins remained to be elucidated. Here, we describe the mechanism and further applications of Potato virus X (PVX)-based VIF in the short-day Nicotiana tabacum cultivar Maryland Mammoth. Ectopic delivery of Arabidopsis (Arabidopsis thaliana) AtFT by PVX/AtFT did not induce the expression of the endogenous FT ortholog NtFT4; however, it was sufficient to trigger flowering in Maryland Mammoth plants grown under noninductive long-day conditions. Infected tobacco plants developed no systemic symptoms, and the PVX-based VIF did not cause transgenerational flowering. We showed that the PVX-based VIF is a much more rapid method to examine the impacts of single amino acid mutations on AtFT for floral induction than making individual transgenic Arabidopsis lines for each mutation. We also used the PVX-based VIF to demonstrate that adding a His-or FLAG-tag to the N or C terminus of AtFT could affect its florigenic activity and that this system can be applied to assay the function of FT genes from heterologous species, including tomato (Solanum lycopersicum) SFT and rice (Oryza sativa) Hd3a. Thus, the PVX-based VIF represents a simple and efficient system to identify individual amino acids that are essential for FT-mediated floral induction and to test the ability of mono-and dicotyledonous FT genes and FT fusion proteins to induce flowering.Modified plant viruses have emerged as powerful tools for dissecting gene function in plants. Such plant virus-based technology can be applied to facilitate or impede gene expression, resulting in gain-or loss-offunction phenotypes. Although virus-based technology was initially exploited for the purpose of high-level production of foreign proteins, such as recombinant subunit vaccines and pharmaceutical proteins for molecular pharming (Scholthof et al., 1996;Porta and Lomonossoff, 2002), plant RNA and DNA virus-based techniques such as small interfering RNA-mediated virus-induced posttranscriptional gene silencing (VIGS) has been extensively utilized to silence genes for functional genomic studies in dicots and monocots, including plants and crops recalcitrant to classical forward or reverse genetic manipulation (Lindbo et al

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Section: Discussionmentioning

confidence: 99%

Section: Pvx-based Vifmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A Virus-Induced Assay for Functional Dissection and Analysis of Monocot and Dicot Flowering Time Genes

Cheng

Chen²,

Shen³

et al. 2017

Plant Physiol.

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Phloem Long‐Distance Trafficking of RNAs and Proteins

Xoconostle‐Cázares

Martínez-Navarro

Ruíz-Medrano

2016

Encyclopedia of Life Sciences

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The vascular tissue has been essential for the evolutionary radiation of land plants, and has allowed them to colonise a wide range of habitats. The phloem is involved in the distribution of fixed carbon throughout the whole plant. The concept of this tissue as a central distributor of signals that regulate plant development and responses to environmental cues has emerged recently. Indeed, several phenomena, such as post‐transcriptional gene silencing, flowering and tuber induction, as well as systemic acquired resistance to pathogens rely on signals, originating in source leaves, and transported via the phloem to systemic tissues. Considering that the phloem translocation stream contains a large array of macromolecules. Given that the functional phloem consists of interconnected enucleate cells, the sieve elements (SEs), the majority of proteins and ribonucleic acids (RNAs) found in phloem sap exudates must originate from the adjoining companion cell (CC). While several proteins play a role in the maintenance of the SEs, it is possible that several species of proteins and RNAs function in long‐distance signalling. In this article the current state of the art of this subject is described, as well as perspectives and possible applications for crop improvement. Key Concepts The vascular tissue consists of xylem and phloem. The xylem is composed of vessels that are dead cells upon maturity that transport mineral nutrients from roots to shoots. The phloem is composed of vessels formed from enucleated living cells involved in the transport of fixed carbon from photosynthetic to heterotrophic organs. The phloem is involved in the delivery of signals that control development as well as responses to external stimuli, such as defence response, post‐transcriptional gene silencing and response to phosphate starvation. Viruses and viroids are transported through the phloem during systemic infection. These probably take advantage of a system enabling long‐distance transport of endogenous proteins and RNAs. The phloem translocation stream contains a wide variety of proteins and RNAs, some of which appear to have a signalling function. RNA and protein exchange can occur between plant pathogenic fungi and parasitic plants and their hosts. Knowledge of this RNA and protein phloem transport system can have applications for crop improvement.

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Nucleic acids movement and its relation to genome dynamics of repetitive DNA

Kejnovský

Jedlička

2022

BioEssays

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There is growing evidence of evolutionary genome plasticity. The evolution of repetitive DNA elements, the major components of most eukaryotic genomes, involves the amplification of various classes of mobile genetic elements, the expansion of satellite DNA, the transfer of fragments or entire organellar genomes and may have connections with viruses. In addition to various repetitive DNA elements, a plethora of large and small RNAs migrate within and between cells during individual development as well as during evolution and contribute to changes of genome structure and function.Such migration of DNA and RNA molecules often results in horizontal gene transfer, thus shaping the whole genomic network of interconnected species. Here, we propose that a high evolutionary dynamism of repetitive genome components is often related to the migration/movement of DNA or RNA molecules. We speculate that the cytoplasm is probably an ideal compartment for such evolutionary experiments.

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Mobile FT mRNA contributes to the systemic florigen signalling in floral induction

Cited by 88 publications

References 39 publications

A Virus-Induced Assay for Functional Dissection and Analysis of Monocot and Dicot Flowering Time Genes

A Virus-Induced Assay for Functional Dissection and Analysis of Monocot and Dicot Flowering Time Genes

Phloem Long‐Distance Trafficking of RNAs and Proteins

Nucleic acids movement and its relation to genome dynamics of repetitive DNA

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