Mobile Colistin Resistance Gene mcr-1 Detected on an IncI2 Plasmid in Salmonella Typhimurium Sequence Type 19 from a Healthy Pig in South Korea

Moon, Dong Chan; Kim, Su-Jeong; Mechesso, Abraham Fikru; Kang, Hee Young; Song, Haa‐Na; Choi, Ji-Hyun; Yoon, Sang-Hyub; Lim, Suk-Kyung

doi:10.3390/microorganisms9020398

Cited by 14 publications

(11 citation statements)

References 52 publications

(83 reference statements)

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“…In these ceftriaxone-resistant Salmonella isolates, we found two mcr -1-positive isolates; interestingly, one of them was colistin susceptible, with a truncated mcr -1 allele. mcr -1-positive Salmonella isolates have been previously reported in Brazil ( Rau et al, 2020 ), Spain ( Trujillo-Soto et al, 2019 ), South Korea ( Moon et al, 2021 ), and China ( Lu et al, 2019 ), and the mcr -1 gene was found to be normally located on IncI2, IncHI2, and IncX4 plasmid types, respectively.…”

Section: Discussionmentioning

confidence: 98%

Prevalence and Characteristics of Ceftriaxone-Resistant Salmonella in Children’s Hospital in Hangzhou, China

Shi

Lan

et al. 2021

Front. Microbiol.

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The non-Typhi Salmonella (NTS) infection is critical to children’s health, and the ceftriaxone is the important empirical treatment choice. With the increase resistance rate of ceftriaxone in Salmonella, the molecular epidemiology and resistance mechanism of ceftriaxone-resistant Salmonella needs to be studied. From July 2019 to July 2020, a total of 205 NTS isolates were collected, 195 of which (95.1%) were cultured from stool, but 10 isolates were isolated from an extraintestinal site. Serogroup B accounted for the vast majority (137/205) among the isolates. Fifty-three isolates were resistant to ceftriaxone, and 50 were isolated from children younger than 4years of age. The resistance rates for ceftriaxone, ciprofloxacin, and levofloxacin were significantly higher in younger children than the older children. The resistance genes in the ceftriaxone-susceptible isolates were detected by PCR, and ceftriaxone-resistant Salmonella were selected for further whole-genome sequencing. Whole-genome analysis showed that serotype Typhimurium and its monophasic variant was the most prevalent in ceftriaxone-resistant isolates (37/53), which comprised ST34 (33/53), ST19 (2/53), and ST99 (2/53), and they were close related in the phylogenetic tree. However, the other isolates were diverse, which included one Enteritidis (ST11), one Indiana (ST17), one Derby (ST40), four Kentucky (ST198), two Goldcoast (ST2529, ST358), one Muenster (ST321), one Virchow (ST359), one Rissen (ST469), one Kedougou (ST1543), two Uganda (ST684), and one Kottbus (ST8839). Moreover, CTX-M-55 ESBLs production (33/53) was found to be mainly responsible for ceftriaxone resistance, followed by blaCTX-M-65 (12/53), blaCTX-M-14 (4/53), blaCTX-M-9 (2/53), blaCTX-M-64 (1/53), blaCTX-M-130 (1/53), and blaCMY-2 (1/53). ISEcp1, IS903B, IS Kpn26, IS1F, and IS26 were connected to antimicrobial resistance genes transfer. In conclusion, the dissemination of ESBL-producing Salmonella isolates resulted in an increased prevalence of ceftriaxone resistance in young children. The high rate of multidrug resistance should be given additional attention.

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Section: Discussionmentioning

confidence: 98%

Prevalence and Characteristics of Ceftriaxone-Resistant Salmonella in Children’s Hospital in Hangzhou, China

Shi

Lan

et al. 2021

Front. Microbiol.

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“…Comparisons with previously published Salmonella and E.coli IncI2 plasmids (Table S1), carrying mcr-1.1 gene, are shown in Figure 2. IncI2 plasmid pS7627-2 has a similar plasmid backbone and a similar genetic environment surrounding the mcr-1.1 gene (nikB-mcr-1.1-PAP) compared to other IncI2 plasmids (pCESA664-3, pCESA244-2, pK18JST013) previously isolated in China and Korea [31]. The ISApl1 insertion sequence belonging to IS30 family was not identified near mcr-1.1.…”

Section: Salmonella Bovismorbificans Slr1_7627mentioning

confidence: 94%

Mobile Colistin Resistance Genetic Determinants of Non-typhoid <em>Salmonella enterica</em> Isolates from Russia

Kuleshov¹,

Pavlova²,

Shedko³

et al. 2021

Preprint

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Polymyxin resistance, determined by mcr genes located on plasmid DNA, currently pose a high epidemiological threat. Non-typhoid Salmonella (NTS) are one of the key pathogens causing diarrheal diseases. Here, we report the isolation and whole genome sequencing of multidrug colistin-resistant/susceptible isolates of non-typhoid Salmonella enterica serovars carries mcr genes. Non-typhoid strains of Salmonella enterica subsp. enterica were isolated during microbiological monitoring of the environment, food, and diarrheal disease patients between 2018 and 2020 in Russia (n=586). mcr-1 genes were detected using a previously developed qPCR assay and whole genome sequencing of mcr positive isolates was performed by both short-read (Illumina) and long-read (Oxford Nanopore) approaches. Three colistin-resistant isolates including two isolates of S. Enteritidis and one isolate of S. Bovismorbificans carried the mcr-1.1 gene located on IncX4 and IncI2 conjugative plasmids, respectively. The phenotypically colistin-susceptible isolate of S. Typhimurium carried a mcr-9 gene on plasmid IncHI2. In conclusion, we present the first three cases of mcr gene carrying NTS isolates detected in Russia with both outbreak and sporadic epidemiological background.

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“…A PCR assay was performed to detect the most frequently reported carbapenem resistance genes in South Korea (bla IMP , bla VIM , bla OXA-48 -like, bla NDM , and bla KPC ) (Table S2) [29,30]. Whole-genome sequencing was performed using PacBio RS II (Pacific Biosciences, Menlo Park, CA, USA), as previously described [31]. Antimicrobial resistance genes were analyzed by the Center for Genomic Epidemiology (http://www.genomicepidemiology.org/; accessed on 18 February 2021).…”

Section: Polymerase Chain Reaction (Pcr) and Whole-genome Sequencingmentioning

confidence: 99%

Imipenem Resistance Mediated by blaOXA-913 Gene in Pseudomonas aeruginosa

Moon¹,

Mechesso²,

Kang³

et al. 2021

Antibiotics

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Treatment of infectious diseases caused by carbapenem-resistant Pseudomonas aeruginosa is becoming a greater challenge. This study aimed to identify the imipenem resistance mechanism in P. aeruginosa isolated from a dog. Minimum Inhibitory Concentration (MIC) was determined by the broth microdilution method according to the Clinical and Laboratory Standards Institute recommendations. We performed polymerase chain reaction and whole-genome sequencing to detect carbapenem resistance genes. Genomic DNA of P. aeruginosa K19PSE24 was sequenced via the combined analysis of 20-kb PacBio SMRTbell and PacBio RS II. Peptide-Peptide Nucleic Acid conjugates (P-PNAs) targeting the translation initiation region of blaOXA-913 were synthesized. The isolate (K19PSE24) was resistant to imipenem and piperacillin/tazobactam yet was susceptible to most of the tested antimicrobials. Whole-genome sequencing revealed that the K19PSE24 genome comprised a single contig amounting to 6,815,777 base pairs, with 65 tRNA and 12 rRNA genes. K19PSE24 belonged to sequence type 313 and carried the genes aph(3)-IIb, fosA, catB7, crpP, and blaOXA-913 (an allele deposited in GenBank but not described in the literature). K19PSE24 also carried genes encoding for virulence factors (exoenzyme T, exotoxin A, and elastase B) that are associated with adhesion, invasion, and tissue lysis. Nevertheless, we did not detect any of the previously reported carbapenem resistance genes. This is the first report of the blaOXA-913 gene in imipenem-resistant P. aeruginosa in the literature. Notably, no viable colonies were found after co-treatment with imipenem (2 µg/mL) and either of the P-PNAs (12.5 µM or 25 µM). The imipenem resistance in K19PSE24 was primarily due to blaOXA-913 gene carriage.

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Mobile Colistin Resistance Gene mcr-1 Detected on an IncI2 Plasmid in Salmonella Typhimurium Sequence Type 19 from a Healthy Pig in South Korea

Cited by 14 publications

References 52 publications

Prevalence and Characteristics of Ceftriaxone-Resistant Salmonella in Children’s Hospital in Hangzhou, China

Prevalence and Characteristics of Ceftriaxone-Resistant Salmonella in Children’s Hospital in Hangzhou, China

Mobile Colistin Resistance Genetic Determinants of Non-typhoid <em>Salmonella enterica</em> Isolates from Russia

Imipenem Resistance Mediated by blaOXA-913 Gene in Pseudomonas aeruginosa

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