Mobile and immobile adhesion of staphylococcal strains to hydrophilic and hydrophobic surfaces

Boks, Niels P.; Kaper, Hans J.; Norde, Willem; Mei, Henny C. van der; Busscher, Henk J.

doi:10.1016/j.jcis.2008.11.025

Cited by 48 publications

(29 citation statements)

References 28 publications

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“…Subsequently, the coated wafer was thoroughly rinsed with methanol and ultrapure water (18 MΩ·cm water, NANOpure Diamond, Dubuque, IA, USA) and stored in a clean capped vial until use. This treatment is known to render a hydrophobic surface with a water contact angle around 100° [29]. Pluronic F-127 was purchased from Sigma (Saint Louis, MO, USA) and used to prepare antifouling coatings on hydrophobic substrata.…”

Section: Methodsmentioning

confidence: 99%

Staining proteins: A simple method to increase the sensitivity of ellipsometric measurements in adsorption studies

Nejadnik

Garcı́a

2011

Colloids and Surfaces B: Biointerfaces

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This communication describes a simple way to improve the sensitivity of spectroscopic ellipsometry, when applied to monitor the adsorption of proteins to solid surfaces. The method described herein is based on the reaction of a commercially available dye (Coomassie brilliant blue G) with the adsorbed proteins and the subsequent analysis by spectroscopic ellipsometry. In order to demonstrate the potential advantages of this method, the adsorption of bovine serum albumin to an antifouling coating was also investigated. According to our results, the modification with the dye significantly affects the optical properties of the adsorbed protein layer, which can be represented using a simple optical model (Lorentz). In general, the proposed modification increases the sensitivity of the detection by 2.5 ± 0.4 fold and enables the analysis of thin layers of adsorbed protein not obtainable by conventional methods. These results particularly reveal the importance of the proposed modification for the evaluation of low adsorbing substrates and antifouling coatings.

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Section: Methodsmentioning

confidence: 99%

Staining proteins: A simple method to increase the sensitivity of ellipsometric measurements in adsorption studies

Nejadnik

Garcı́a

2011

Colloids and Surfaces B: Biointerfaces

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“…The surface properties of biomaterials or medical devices can be changed by coating application or surface modification to create the desired anti-adhesion characteristics without altering the bulk properties of materials. These surface properties include chemical composition and reactivity, hydrophilicity and hydrophobicity [60], surface roughness [61,62] or texture [63], and surface charge. Following this approach, our research team has developed trimethylsilane (TMS) plasma nanocoatings using low temperature plasma coating technology to coat surfaces of stainless steel and titanium for reduced bacterial adhesion and biofilm formation [64].…”

Section: Bioengineering Approachesmentioning

confidence: 99%

Novel Strategies for the Prevention and Treatment of Biofilm Related Infections

Chen

Sun

2013

IJMS

370

272

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Biofilm formation by human bacterial pathogens on implanted medical devices causes major morbidity and mortality among patients, and leads to billions of dollars in healthcare cost. Biofilm is a complex bacterial community that is highly resistant to antibiotics and human immunity. As a result, novel therapeutic solutions other than the conventional antibiotic therapies are in urgent need. In this review, we will discuss the recent research in discovery of alternative approaches to prevent or treat biofilms. Current anti-biofilm technologies could be divided into two groups. The first group focuses on targeting the biofilm forming process of bacteria based on our understanding of the molecular mechanism of biofilm formation. Small molecules and enzymes have been developed to inhibit or disrupt biofilm formation. Another group of anti-biofilm technologies focuses on modifying the biomaterials used in medical devices to make them resistant to biofilm formation. While these novel anti-biofilm approaches are still in nascent phases of development, efforts devoted to these technologies could eventually lead to anti-biofilm therapies that are superior to the current antibiotic treatment.

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“…For instance, in vivo , stainless steel is colonized more readily than titanium, perhaps due to differences in osseointegration [82–83] which may reflect differences in protein adsorption to the surface. Hydrophobicity/hydrophilicity, surface composition, and texturing all impact bone ingrowth and bacterial adhesion [84–86]; of these, the most important is surface hydrophobicity [87–89]. Texturing of surfaces at the micro level through processes such as sand-blasting and at the macro level through introduction of beads, sintering, etc., have improved osseointegration of the neck of joint replacement stems [90–93].…”

Section: Implant Designs To Minimize Bacterial Colonizationmentioning

confidence: 99%

Immobilized antibiotics to prevent orthopaedic implant infections

Hickok¹,

Shapiro²

2012

Advanced Drug Delivery Reviews

258

193

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Many surgical procedures require the placement of an inert or tissue-derived implant deep within the body cavity. While the majority of these implants do not become colonized by bacteria, a small percentage develops a biofilm layer that harbors invasive microorganisms. In orthopaedic surgery, unresolved periprosthetic infections can lead to implant loosening, arthrodeses, amputations and sometimes death. The focus of this review is to describe development of an implant in which an antibiotic tethered to the metal surface is used to prevent bacterial colonization and biofilm formation. Building on well-established chemical syntheses, studies show that antibiotics can be linked to titanium through a self-assembled monolayer of siloxy amines. The stable metal-antibiotic construct resists bacterial colonization and biofilm formation while remaining amenable to osteoblastic cell adhesion and maturation. In an animal model, the antibiotic modified implant resists challenges by bacteria that are commonly present in periprosthetic infections. While the long-term efficacy and stability is still to be established, ongoing studies support the view that this novel type of bioactive surface has a real potential to mitigate or prevent the devastating consequences of orthopaedic infection.

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Mobile and immobile adhesion of staphylococcal strains to hydrophilic and hydrophobic surfaces

Cited by 48 publications

References 28 publications

Staining proteins: A simple method to increase the sensitivity of ellipsometric measurements in adsorption studies

Staining proteins: A simple method to increase the sensitivity of ellipsometric measurements in adsorption studies

Novel Strategies for the Prevention and Treatment of Biofilm Related Infections

Immobilized antibiotics to prevent orthopaedic implant infections

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