MNU affects mouse erythroleukemia cell differentiation at sub-cytotoxic doses

Sun-Hoffman, L; Winicov, Ilga

doi:10.1016/0009-2797(96)03702-7

Cited by 2 publications

(2 citation statements)

References 40 publications

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“…Mice administered 75 mg/kg MNU exhibited significantly decreased body weights compared with those administered citrate buffer at 2-3 weeks after dosing. This body weight loss may be caused by the acute toxicity of this chemical [5]. Although the body weight of mice administered MNU gradually recovered, it remained lower than that of citrate buffer control animals at most time points.…”

Section: Body Weight and Haematological And Biochemistry Analysesmentioning

confidence: 93%

“…The generation of adult T-cell leukaemia/lymphoma [3] and human acute B-lymphoblastic leukaemia [4] by xenotransplantation of primary peripheral blood mononuclear cells into combined immunodeficient mice were reported recently. In addition to the use of immunodeficient mice, irradiation or thymectomy have also been used to optimize models [5]. Genetically modified mice have also been studied intensively as potential lymphoma models [6][7][8][9][10] p53 is a well-known tumour suppressor gene, p53 knocked out mice appear spontaneous carcinogenesis [11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

WITHDRAWN: A uniform thymic malignant lymphoma model established with C57BL/6 p53 gene deficient mice

Fan¹,

Lyu²,

Liu³

et al. 2018

Oncotarget

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Lymphoma is the third most common cancer diagnosed in children and T cell lymphoma has the worst prognosis by clinical observation. So far, a lymphoma model with uniform penetrance has not been available. In this paper, we generated a p53 deficient mouse model by targeting embryo stem cells derived from the C57BL/6 mouse strain. Homozygous p53 deficient mice exhibited more accelerated rate of spontaneous tumorigenesis with a high spontaneous occurrence rate (93.3%) of malignant lymphoma. Because tumour models with a high phenotypic consistency are currently needed, we further generated the lymphoma model by single intraperitoneal injection of 37.5 or 75 mg/kg N-methyl-N-nitrosourea (MNU) to p53 deficient mice. Lymphoma and retinal degeneration occurred in 100% of p53 +/mice administrated higher concentration of MNU, which was much higher than previously reported models. The main anatomic sites of lymphoma were thymus, spleen, bone marrow, and lymph nodes. Both induced and spontaneous lymphomas in the thymus and spleen were stained positive for CD3 antigen. Furthermore, positive CD4 and/or positive CD8 cells were detected by flow cytometry, indicating a T-cell lineage of the lymphomas. The onset time of this uniform lymphoma model was from 13 to 17 weeks after the administration of MNU accompanied by the second time of weight loss. Based on our observations and previous data, we hypothesised that mice with the B6 background are prone to lymphomagenesis. This model could be used to study the mechanisms of lymphomagenesis, to select new drugs, and to improve the reproducibility of experimental results.

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Section: Body Weight and Haematological And Biochemistry Analysesmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: A uniform thymic malignant lymphoma model established with C57BL/6 p53 gene deficient mice

Fan¹,

Lyu²,

Liu³

et al. 2018

Oncotarget

View full text Add to dashboard Cite

show abstract

Generation of a uniform thymic malignant lymphoma model with C57BL/6J <i>p53</i> gene deficient mice

Liu

Lyu²,

et al. 2022

J Toxicol Pathol

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：Lymphoma is the third most common cancer diagnosed in children, and T-cell lymphoma has the worst prognosis based on clinical observations. To date, a lymphoma model with uniform penetrance has not yet been developed. In this study, we generated a p53 deficient mouse model by targeting embryonic stem cells derived from a C57BL/6J mouse strain. Homozygous p53 deficient mice exhibited a higher rate of spontaneous tumorigenesis, with a high spontaneous occurrence rate (93.3%) of malignant lymphoma.Because tumor models with high phenotypic consistency are currently needed, we generated a lymphoma model by a single intraperitoneal injection of 37.5 or 75 mg/kg N-methyl-N-nitrosourea to p53 deficient mice. Lymphoma and retinal degeneration occurred in 100% of p53 +/mice administered with higher concentrations of N-methyl-N-nitrosourea, a much greater response than those of previously reported models. The main anatomic sites of lymphoma were the thymus, spleen, bone marrow, and lymph nodes. Both induced and spontaneous lymphomas in the thymus and spleen stained positive for CD3 antigen, and flow cytometry detected positive CD4 and/or CD8 cells. Based on our observations and previous data, we hypothesize that mice with a B6 background are prone to lymphomagenesis.

show abstract

MNU affects mouse erythroleukemia cell differentiation at sub-cytotoxic doses

Cited by 2 publications

References 40 publications

WITHDRAWN: A uniform thymic malignant lymphoma model established with C57BL/6 p53 gene deficient mice

WITHDRAWN: A uniform thymic malignant lymphoma model established with C57BL/6 p53 gene deficient mice

Generation of a uniform thymic malignant lymphoma model with C57BL/6J <i>p53</i> gene deficient mice

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