MMP-2, MMP-8 Expression in gingival tissue of Chronic Periodontitis associated to Type 2 Diabetes Mellitus

Kang, Min Gu; Jeong, Hakyung; Song, Sun Hee; Park, Jin Woo; Suh, Jo Young; Lee, Jae Mok

doi:10.5051/jkape.2005.35.3.661

Cited by 4 publications

(6 citation statements)

References 32 publications

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“…For Western blotting, as previously described technique by Kang 24) frozen tissues were homogenized in RIPA lysis buffer (10 mM EDTA, 0.15M NaCl) with 1:30 diluted protease inhibitor cocktail(Roche, Germany) 25) . The lysates were sonicated 3 times for 10 seconds and centrifuge at 12,000g for 15 minutes.…”

Section: Protein Isolation and Western Blottingmentioning

confidence: 99%

The comparison of inflammatory mediator expression in gingival tissues from human chronic periodontitis patients with and without type 2 diabetes mellitus

Joo

Lee

2007

J Korean Acad Periodontol

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Section: Protein Isolation and Western Blottingmentioning

confidence: 99%

The comparison of inflammatory mediator expression in gingival tissues from human chronic periodontitis patients with and without type 2 diabetes mellitus

Joo

Lee

2007

J Korean Acad Periodontol

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“…According to Kang et al [ 90 ], MMP-8 is the main proteolytic enzyme detected in periodontal tissue that is affected by inflammation, and its source is most likely degranulating neutrophils. Importantly, bacteria associated with the development of periodontitis, such as Treponema denticola and Porphyromonas gingivalis , through the action of the proteases they produce, increase the activity of this enzyme [ 91 ].…”

Section: Importance Of Mmp-8 In Periodontitismentioning

confidence: 99%

“…This was also confirmed by Mohammed et al [ 119 ]. However, it should be noted that a single study indicates that the determination of MMP-8 concentration is not suitable for differentiating the stages of periodontitis [ 90 ]. In addition, in the saliva of people with periodontitis, MMP-8 is found in different forms, i.e., in the active form, bound in complexes, pro-MMP-8 of neutrophilic origin, and mesenchymal origin [ 120 ].…”

Section: Importance Of Mmp-8 In Periodontitismentioning

confidence: 99%

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Importance of Metalloproteinase 8 (MMP-8) in the Diagnosis of Periodontitis

Zalewska,

Ławicka,

Grygorczuk

et al. 2024

IJMS

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Periodontitis is a complex condition. Left untreated, it leads to tooth loss and the need for prosthetic treatment. The incidence of periodontitis is steadily increasing, so new methods are being sought to aid in the diagnosis of the disease. Among the methods postulated is the determination of concentrations of bioactive compounds which include extracellular matrix metalloproteinases (MMPs). These enzymes are present in various structural elements of the stomatognathic system. The most promising enzyme of this group appears to be metalloproteinase 8 (MMP-8). MMP-8 assays are performed in gingival fluid or saliva, and MMP-8 levels have been shown to be higher in patients with periodontitis compared to healthy subjects and correlated with some clinical parameters of the condition and the severity of the disease. In addition, the preliminary usefulness of this enzyme in evaluating the effectiveness of periodontal treatment and doxycycline therapy has been demonstrated. Determination of the active form of MMP-8 (aMMP-8) in oral rinse fluid using off-the-shelf assays shows the highest potential. Despite reports about aMMP-8 and promising data on the role of MMP-8 in periodontal diagnosis, a clear determination of the usefulness of this enzyme requires further research.

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“…For Western blotting, as previously described technique by Kang et al 25) , frozen tissues were homogenized in RIPA lysis buffer (10 mM EDTA, 0.15M NaCl) with 1:30 diluted protease inhibitor cocktail (Roche, German) according to…”

Section: Protein Isolation and Western Blottingmentioning

confidence: 99%

Interrelationship of of Matrix metalloproteinase-13 and Elastase Expression in Human Gingiva with Chronic Periodontitis Associated to Type 2 Diabetes Mellitus

Park

Lee

2006

J Korean Acad Periodontol

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MMP-2, MMP-8 Expression in gingival tissue of Chronic Periodontitis associated to Type 2 Diabetes Mellitus

Cited by 4 publications

References 32 publications

The comparison of inflammatory mediator expression in gingival tissues from human chronic periodontitis patients with and without type 2 diabetes mellitus

The comparison of inflammatory mediator expression in gingival tissues from human chronic periodontitis patients with and without type 2 diabetes mellitus

Importance of Metalloproteinase 8 (MMP-8) in the Diagnosis of Periodontitis

Interrelationship of of Matrix metalloproteinase-13 and Elastase Expression in Human Gingiva with Chronic Periodontitis Associated to Type 2 Diabetes Mellitus

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