MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with Fasciola hepatica and F. gigantica

Valero, M. Adela; Ubeira, Florencio M.; Khoubbane, M; Artigas, Patricio; Muiño, Laura; Mezo, Mercedes; Pérez-Crespo, Ignacio; Periago, María Victoria; Mas‐Coma, Santiago

doi:10.1016/j.vetpar.2008.10.014

Cited by 68 publications

(47 citation statements)

References 16 publications

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“…More recently, an ELISA based on the detection of fluke antigens in the faeces has been developed commercially: the BIO K201 ELISA (Bio-X Diagnostics, Jemelle, Belgium). Although coproantigens are not identified as early in infection as serum antigens, the test can detect infection several weeks before patency, it has the distinct advantage that it indicates current infection and it is very sensitive (Mezo et al, 2004;Valero et al, 2009;Flanagan et al, 2011a,b).…”

Section: Diagnosismentioning

confidence: 99%

Reducing the future threat from (liver) fluke: realistic prospect or quixotic fantasy?

Fairweather

2011

Veterinary Parasitology

136

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Section: Diagnosismentioning

confidence: 99%

Reducing the future threat from (liver) fluke: realistic prospect or quixotic fantasy?

Fairweather

2011

Veterinary Parasitology

136

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“…The cELISA is also advantageous because it requires less processing time and facilitates batch testing. To date, the performance of the cELISA has shown considerable potential for sheep and cattle diagnostics [18,20–23], and in wild boar ( S . scrofa ) [10]; whereas in horses, the cELISA has been attributed only 9% sensitivity and has therefore been deemed unsuitable [22].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Liver Fluke (Fasciola hepatica) in Wild Red Deer (Cervus elaphus): Coproantigen ELISA Is a Practicable Alternative to Faecal Egg Counting for Surveillance in Remote Populations

et al. 2016

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Red deer (Cervus elaphus) are hosts of liver fluke (Fasciola hepatica); yet, prevalence is rarely quantified in wild populations. Testing fresh samples from remote regions by faecal examination (FE) can be logistically challenging; hence, we appraise frozen storage and the use of a coproantigen ELISA (cELISA) for F. hepatica surveillance. We also present cELISA surveillance data for red deer from the Highlands of Scotland. Diagnoses in faecal samples (207 frozen, 146 fresh) were compared using a cELISA and by FE. For each storage method (frozen or fresh), agreement between the two diagnostics was estimated at individual and population levels, where population prevalence was stratified into cohorts (e.g., by sampling location). To approximate sensitivity and specificity, 65 post-slaughter whole liver examinations were used as a reference. At the individual level, FE and cELISA diagnoses agreed moderately (κfrozen = 0.46; κfresh = 0.51), a likely reflection of their underlying principles. At the population level, FE and cELISA cohort prevalence correlated strongly (Pearson’s R = 0.89, p < 0.0001), reflecting good agreement on relative differences between cohort prevalence. In frozen samples, prevalence by cELISA exceeded FE overall (42.8% vs. 25.8%) and in 9/12 cohorts, alluding to differences in sensitivity; though, in fresh samples, no significant difference was found. In 959 deer tested by cELISA across the Scottish Highlands, infection prevalence ranged from 9.6% to 53% by sampling location. We highlight two key advantages of cELISA over FE: i) the ability to store samples long term (frozen) without apparent loss in diagnostic power; and ii) reduced labour and the ability to process large batches. Further evaluation of cELISA sensitivity in red deer, where a range of fluke burdens can be obtained, is desirable. In the interim, the cELISA is a practicable diagnostic for F. hepatica surveillance in red deer, and its application here has revealed considerable geographic, temporal, sex and age related differences in F. hepatica prevalence in wild Scottish Highland red deer.

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“…In previous studies with the MM3-SERO test in sheep [27], we have observed that such a test can be used to detect infections by either F. hepatica or F. gigantica . In this study we did not have the opportunity to test sera from patients infected with the latter species.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, cathepsins L are the most frequently used target antigens for detecting anti- Fasciola antibodies [15], [21], [25], [26], as circulating antibodies to these molecules remain at high levels for long periods [27]. An ELISA test (MM3-SERO) that had proven useful for serodiagnosis of fasciolosis in several animal species with maximal sensitivity and specificity [27]–[30], was recently found to involve binding to cathepsins L1 and L2 from both species of Fasciola [31].…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis

et al. 2011

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BackgroundHuman fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katz test, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate for detection of acute infections, ectopic infections, or infections with low number of parasites. In such cases immunological methods may be a good alternative and are recommended for use in major hospitals where trained personnel are available, although they are not usually implemented for individual testing.Methodology/Principal FindingsWe have developed a new lateral flow test (SeroFluke) for the serodiagnosis of human fasciolosis. The new test was constructed with a recombinant cathepsin L1 from F. hepatica, and uses protein A and mAb MM3 as detector reagents in the test and control lines, respectively. In comparison with an ELISA test (MM3-SERO) the SeroFluke test showed maximal specificity and sensitivity and can be used with serum or whole blood samples.Conclusions/SignificanceThe new test can be used in major hospitals in hypoendemic countries as well as in endemic/hyperendemic regions where point-of-care testing is required.

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MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with Fasciola hepatica and F. gigantica

Cited by 68 publications

References 16 publications

Reducing the future threat from (liver) fluke: realistic prospect or quixotic fantasy?

Reducing the future threat from (liver) fluke: realistic prospect or quixotic fantasy?

Prevalence of Liver Fluke (Fasciola hepatica) in Wild Red Deer (Cervus elaphus): Coproantigen ELISA Is a Practicable Alternative to Faecal Egg Counting for Surveillance in Remote Populations

Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis

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