Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis

Martínez-Sernández, Victoria; Muiño, Laura; Perteguer, María Jesús; Gárate, Teresa; Mezo, Mercedes; González-Warleta, Marta; Muro, Antonio; Costa, José Manuel Correia da; Romarís, F.; Ubeira, Florencio M.

doi:10.1371/journal.pntd.0001376

Cited by 62 publications

(31 citation statements)

References 40 publications

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“…Martínez‐Sernández et al. () developed a lateral flow test (SeroFluke) for the serodiagnosis of human fasciolosis. In comparison with an ELISA test (MM3‐SERO), the SeroFluke test showed excellent specificity and sensitivity and could be used with serum or whole blood samples.…”

Section: Diagnosismentioning

confidence: 99%

Fasciola and fasciolosis in ruminants in Europe: Identifying research needs

Beesley

Caminade

Charlier

et al. 2017

Transbound Emerg Dis

157

111

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Summary Fasciola hepatica is a trematode parasite with a global distribution, which is responsible for considerable disease and production losses in a range of food producing species. It is also identified by WHO as a re‐emerging neglected tropical disease associated with endemic and epidemic outbreaks of disease in human populations. In Europe, F. hepatica is mostly associated with disease in sheep, cattle and goats. This study reviews the most recent advances in our understanding of the transmission, diagnosis, epidemiology and the economic impact of fasciolosis. We also focus on the impact of the spread of resistance to anthelmintics used to control F. hepatica and consider how vaccines might be developed and applied in the context of the immune‐modulation driven by the parasite. Several major research gaps are identified which, when addressed, will contribute to providing focussed and where possible, bespoke, advice for farmers on how to integrate stock management and diagnosis with vaccination and/or targeted treatment to more effectively control the parasite in the face of increasing the prevalence of infection and spread of anthelmintic resistance that are likely to be exacerbated by climate change.

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Section: Diagnosismentioning

confidence: 99%

Fasciola and fasciolosis in ruminants in Europe: Identifying research needs

Beesley

Caminade

Charlier

et al. 2017

Transbound Emerg Dis

157

111

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“…Similar ELISA tests are available for F. gigantica using a variety of different antigens: (a) Fas1 and Fas2 cysteine proteases, resulting in 91·9% and 89·1%, sensitivity and specificity, respectively ; (b) sandwich ELISA using fatty acid binding protein, resulting in 94·7% sensitivity and 84·62% specificities ; (c) 27 kDa circulating antigen from sera of infected individuals gave >93% sensitivity and specificity . A lateral flow test (SeroFluke) was developed by Martinez‐Sernandez and colleagues using samples obtained from F. hepatica‐ infected patients in Spain and Portugal, for use with serum or whole blood samples, which requires minimal training . However, ELISAs capable of distinguishing active infection by F. hepatica and F. gigantica are needed for diagnosis in areas of geographical overlap of the two species, particularly if access to the adult flukes for genotyping is not available.…”

Section: Diagnosis Of Human Liver Fluke Infectionmentioning

confidence: 99%

A prospective view of animal and human Fasciolosis

Cwiklinski

O’Neill

Donnelly

et al. 2016

Parasite Immunology

167

114

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SummaryFasciolosis, a food‐borne trematodiasis, results following infection with the parasites, Fasciola hepatica and Fasciola gigantica. These trematodes greatly affect the global agricultural community, infecting millions of ruminants worldwide and causing annual economic losses in excess of US $3 billion. Fasciolosis, an important zoonosis, is classified by WHO as a neglected tropical disease with an estimated 17 million people infected and a further 180 million people at risk of infection. The significant impact on agriculture and human health together with the increasing demand for animal‐derived food products to support global population growth demonstrate that fasciolosis is a major One Health problem. This review details the problematic issues surrounding fasciolosis control, including drug resistance, lack of diagnosis and the threat that hybridization of the Fasciola species poses to future animal and human health. We discuss how these parasites may mediate their long‐term survival through regulation and modulation of the host immune system, by altering the host immune homeostasis and/or by influencing the intestinal microbiome particularly in respect to concurrent infections with other pathogens. Large genome, transcriptome and proteomic data sets are now available to support an integrated One Health approach to develop novel diagnostic and control strategies for both animal and human disease.

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“…To date, various diagnostic techniques for the identification and discrimination of Fasciola spp. have been developed, including the traditional detection of eggs in feces directly or after Kato-Katz based sedimentation (19,43), the morphological examination of adults (38), the lateral flow immunoassay for serodiagnosis (28), and the enzyme-linked immunosorbent assay (indirect ELISA) (12,13,32,36).…”

mentioning

confidence: 99%

Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

Nguyen

et al. 2012

J Clin Microbiol

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A single-step multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica. These species overlap in distribution in many countries of North and East Africa and Central and Southeast Asia and are similar in egg morphology, making identification from fecal samples difficult. Based on a comparative alignment of mitochondrial DNA (mtDNA) spanning the region of cox1-trnT-rrnL, two species-specific forward primers were designed, FHF (for F. hepatica) and FGF (for F. gigantica), and a single reverse primer, FHGR (common for both species). Conventional PCR followed by sequencing was applied using species-specific primer pairs to verify the specificity of primers and the identity of Fasciola DNA templates. Duplex PCR (using three primers) was used for testing with the DNA extracted from adult worms, miracidia, and eggs, producing amplicons of 1,031 bp for F. hepatica and 615 bp for F. gigantica. The duplex PCR failed to amplify from DNA of other common liver and intestinal trematodes, including two opisthorchiids, three heterophyids, an echinostomid, another fasciolid, and a taeniid cestode. The sensitivity assay showed that the duplex PCR limit of detection for each Fasciola species was between 0.012 ng and 0.006 ng DNA. Evaluation using DNA templates from 32 Fasciola samples (28 adults and 4 eggs) and from 25 field-collected stools of ruminants and humans revealed specific bands of the correct size and the presence of Fasciola species. This novel mtDNA duplex PCR is a sensitive and fast tool for accurate identification of Fasciola species in areas of distributional and zonal overlap.

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Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis

Cited by 62 publications

References 40 publications

Fasciola and fasciolosis in ruminants in Europe: Identifying research needs

Fasciola and fasciolosis in ruminants in Europe: Identifying research needs

A prospective view of animal and human Fasciolosis

Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

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