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Vl, Chubykin

doi:10.1023/a:1009058323327

Cited by 6 publications

(4 citation statements)

References 46 publications

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“…We performed the cytological analysis of the oocyte nucleus in mutant lawc p1 /l(1)EF520 females (the frequency of X-chromosome nondisjunction is 5.2%) with low Trf2 expression. Squash preparations of ovaries were prepared by modified Puro and Nokkala method [10,22].…”

Section: Study Of the Origin Of Chromosome Nondisjunction In Lawc Mutmentioning

confidence: 99%

“…The chromocenter is generated by the association of pericentromeric regions of all or separate groups of chromosomes and plays an important role in spatial organization of chromosomes [8]. Studies on Drosophila have clearly demonstrated that its disorganization leads to genomic disbalance [9,10]. The screening for genes that control the formation and reorganization of chromocenter is performed [11,12].…”

Section: Introductionmentioning

confidence: 99%

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The Effect of TBP-Related Factor 2 on Chromocenter Formation and Chromosome Segregation in Drosophila Melanogaster

Vorontsova¹,

Cherezov²,

Симонова³

2017

Chromosomal Abnormalities - A Hallmark Manifestation of Genomic Instability

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Chromosome nondisjunction in meiosis causes the gene disbalance and a number of anomalies in development and fertility. Otherwise, genetically programmed sex-ratio meiotic drive occurs in a number of species. One of the forms of eukaryotic genome organization is a chromocenter evolutionally involved in the regulation of chromosome behavior in dividing cells among insects, plants, mammals, mollusks, and even yeast. In Drosophila, TBP related factor 2 (Trf2) belongs to a conservative Tbp (TATA box-binding protein) gene family and encodes a basic transcription factor. Recent data demonstrates that a decrease in TRF2 expression can result in the abnormalities of chromatin condensation; however, no details of this process have been studied. We demonstrated that a decrease in the TRF2 expression damaged proper chromocenter structure and abolished chromatin condensation and it was a reason for the chromosome nondisjunction. We found that compact chromocenter and correct homologue pairing were abolished in flies with a lower Trf2 expression in germline and in somatic cells. We conclude that TRF2 can not only be involved in transcription activation, but also may perform structural function in pericentromeric heterochromatin organization. The possibility of TRF2 to regulate the evolutionary genetically programmed sex-ratio meiotic drive is discussed.

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Section: Study Of the Origin Of Chromosome Nondisjunction In Lawc Mutmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effect of TBP-Related Factor 2 on Chromocenter Formation and Chromosome Segregation in Drosophila Melanogaster

Vorontsova¹,

Cherezov²,

Симонова³

2017

Chromosomal Abnormalities - A Hallmark Manifestation of Genomic Instability

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“…Although unclear, the biological function of heterochromatic associations may be multifaceted. Among others, creating nuclear order/constraints, arranging of chromosome territories and nuclear compartmentation, regulating the functional activity of the nucleus, genome guarding and packaging, and meiotic segregation, and facilitating homologous chromosome recognition and juxtaposition have been postulated (Fussell 1987;Dernburg et al 1996;Ceccarelli et al 1998;Chubykin 2001;Wijchers et al 2015;Ostromyshenskii et al 2018 and literature therein;Jagannathan et al 2018;Falk et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Pericentromere clustering in Tradescantia section Rhoeo involves self-associations of AT- and GC-rich heterochromatin fractions, is developmentally regulated, and increases during differentiation

2020

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A spectacular but poorly recognized nuclear repatterning is the association of heterochromatic domains during interphase. Using basespecific fluorescence and extended-depth-of-focus imaging, we show that the association of heterochromatic pericentromeres composed of AT-and GC-rich chromatin occurs on a large scale in cycling meiotic and somatic cells and during development in ring-and bivalent-forming Tradescantia spathacea (section Rhoeo) varieties. The mean number of pericentromere AT-rich domains per root meristem nucleus was ca. half the expected diploid number in both varieties, suggesting chromosome pairing via (peri)centromeric regions. Indeed, regular pairing of AT-rich domains was observed. The AT-and GC-rich associations in differentiated cells contributed to a significant reduction of the mean number of the corresponding foci per nucleus in relation to root meristem. Within the first 10 mm of the root, the pericentromere attraction was in progress, as if it was an active process and involved both AT-and GC-rich associations. Complying with Rabl arrangement, the pericentromeres preferentially located on one nuclear pole, clustered into diverse configurations. Among them, a strikingly regular one with 5-7 ring-arranged pericentromeric AT-rich domains may be potentially engaged in chromosome positioning during mitosis. The fluorescent pattern of pachytene meiocytes and somatic nuclei suggests the existence of a highly prescribed ring/chain type of chromocenter architecture with side-by-side arranged pericentromeric regions. The dynamics of pericentromere associations together with their non-random location within nuclei was compared with nuclear architecture in other organisms, including the widely explored Arabidopsis model.

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“…Studies on Drosophila and mice have clearly demonstrated that its disorganization leads to genomic disbalance. It has been suggested that the chromocenter performs coordination of chromosome before division and indirectly increases the frequency of crossing over when its forming is delayed [22][23][24]. A detailed study of chromocenters in Drosophila oocytes has shown that their structure is genetically programmed [25,26].…”

Section: Introductionmentioning

confidence: 99%

TBP-Related Factor 2 as a Trigger for Robertsonian Translocations and Speciation

Cherezov

Vorontsova

Симонова

2020

IJMS

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Robertsonian (centric-fusion) translocation is the form of chromosomal translocation in which two long arms of acrocentric chromosomes are fused to form one metacentric. These translocations reduce the number of chromosomes while preserving existing genes and are considered to contribute to speciation. We asked whether hypomorphic mutations in genes that disrupt the formation of pericentromeric regions could lead to centric fusion. TBP-related factor 2 (Trf2) encodes an alternative general transcription factor. A decrease of TRF2 expression disrupts the structure of the pericentromeric regions and prevents their association into chromocenter. We revealed several centric fusions in two lines of Drosophila melanogaster with weak Trf2 alleles in genetic experiments. We performed an RNAi-mediated knock-down of Trf2 in Drosophila and S2 cells and demonstrated that Trf2 upregulates expression of D1—one of the major genes responsible for chromocenter formation and nuclear integrity in Drosophila. Our data, for the first time, indicate that Trf2 may be involved in transcription program responsible for structuring of pericentromeric regions and may contribute to new karyotypes formation in particular by promoting centric fusion. Insight into the molecular mechanisms of Trf2 function and its new targets in different tissues will contribute to our understanding of its phenomenon.

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Cited by 6 publications

References 46 publications

The Effect of TBP-Related Factor 2 on Chromocenter Formation and Chromosome Segregation in Drosophila Melanogaster

The Effect of TBP-Related Factor 2 on Chromocenter Formation and Chromosome Segregation in Drosophila Melanogaster

Pericentromere clustering in Tradescantia section Rhoeo involves self-associations of AT- and GC-rich heterochromatin fractions, is developmentally regulated, and increases during differentiation

TBP-Related Factor 2 as a Trigger for Robertsonian Translocations and Speciation

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