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Masmoudi, R.; Rival, Alain; Nato, A.; Lavergne, D.; Drira, Noureddine; Ducreux, G.

doi:10.1023/a:1006308529895

Cited by 11 publications

(2 citation statements)

References 15 publications

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“…2b, arrows). This protein of about 55 kDa is typically a major component of leaf protein extracts [3,4,34,35]. However, extracts varied in the set of protein bands present in gels (Fig.…”

Section: Sds-page Analysismentioning

confidence: 99%

Protein extraction from Phoenix dactylifera L. leaves, a recalcitrant material, for two‐dimensional electrophoresis

et al. 2008

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This work was aimed at optimizing a protein extraction procedure for date palm (Phoenix dactylifera L.) leaves, a highly recalcitrant plant tissue for 2-DE. Five protein extraction protocols based on different protein precipitation agents (TCA/acetone vs. phenol (Ph) methods) and protein resolubilization methods (physical treatments, e.g., sonication, shaking and/or heating) were tested. Ph/SDS extraction with methanol/ammonium acetate precipitation, followed by DOC preincubation and TCA/acetone precipitation and, finally, solubilization by shaking in rehydration solution was found to be the best protein extraction method. We conclude that DOC with TCA/acetone precipitation step eliminates interfering compounds, thus allowing efficient resolubilization of date palm leaf proteins. This method could be appropriate for proteomic studies such as date palm colonization by entomopathogenic fungi.

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“…2b, arrows). This protein of about 55 kDa is typically a major component of leaf protein extracts [3,4,34,35]. However, extracts varied in the set of protein bands present in gels (Fig.…”

Section: Sds-page Analysismentioning

confidence: 99%

Protein extraction from Phoenix dactylifera L. leaves, a recalcitrant material, for two‐dimensional electrophoresis

et al. 2008

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“…Rubisco and PEPC are key enzymes affecting photosynthesis in plants [ 73 ]. Rubisco catalyzes the carboxylation and oxygenation reactions of ribulose-1,5-bisphosphate (RuBP) and controls photosynthetic carbon metabolism and photorespiration in plants [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Regulatory effects of silicon nanoparticles on the growth and photosynthesis of cotton seedlings under salt and low-temperature dual stress

Liang,

Liu,

et al. 2023

BMC Plant Biol

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Background Silicon nanoparticles (SiO2-NPs) play a crucial role in plants mitigating abiotic stress. However, the regulatory mechanism of SiO2-NPs in response to multiple stress remains unclear. The objectives of this study were to reveal the regulatory mechanism of SiO2-NPs on the growth and photosynthesis in cotton seedlings under salt and low-temperature dual stress. It will provide a theoretical basis for perfecting the mechanism of crop resistance and developing the technology of cotton seedling preservation and stable yield in arid and high salt areas. Results The results showed that the salt and low-temperature dual stress markedly decreased the plant height, leaf area, and aboveground biomass of cotton seedlings by 9.58%, 15.76%, and 39.80%, respectively. While SiO2-NPs alleviated the damage of the dual stress to cotton seedling growth. In addition to reduced intercellular CO2 concentration, SiO2-NPs significantly improved the photosynthetic rate, stomatal conductance, and transpiration rate of cotton seedling leaves. Additionally, stomatal length, stomatal width, and stomatal density increased with the increase in SiO2-NPs concentration. Notably, SiO2-NPs not only enhanced chlorophyll a, chlorophyll b, and total chlorophyll content, but also slowed the decrease of maximum photochemical efficiency, actual photochemical efficiency, photochemical quenching of variable chlorophyll, and the increase in non-photochemical quenching. Moreover, SiO2-NPs enhanced the activities of ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase, improved leaf water potential, and decreased abscisic acid and malondialdehyde content. All the parameters obtained the optimal effects at a SiO2-NPs concentration of 100 mg L− 1, and significantly increased the plant height, leaf area, and aboveground biomass by 7.68%, 5.37%, and 43.00%, respectively. Furthermore, significant correlation relationships were observed between photosynthetic rate and stomatal conductance, stomatal length, stomatal width, stomatal density, chlorophyll content, maximum photochemical efficiency, actual photochemical efficiency, photochemical quenching of variable chlorophyll, and Rubisco activity. Conclusion The results suggested that the SiO2-NPs improved the growth and photosynthesis of cotton seedlings might mainly result from regulating the stomatal state, improving the light energy utilization efficiency and electron transport activity of PSII reaction center, and inducing the increase of Rubisco activity to enhance carbon assimilation under the salt and low-temperature dual stress.

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