Date Palm Micropropagation via Somatic Embryogenesis

Fki, Lotfi; Masmoudi, R.; Kriaâ, Walid; Mahjoub, Ali Ridha; Sghaier, Besma; Mzid, Rim; Mliki, Ahmed; Rival, Alain; Drira, Noureddine

doi:10.1007/978-94-007-1318-5_4

Cited by 44 publications

(32 citation statements)

References 59 publications

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“…The process of somatic embryogenesis comprises a sequence of stages: embryogenic callus induction, somatic embryo formation (somatic embryogenesis expression), somatic embryo maturation and conversion into plantlet. Somatic embryogenesis is the most commonly used technique for date palm micropropagation, mainly for its high plant production potential [1,9]. However, the success of this technique depends on several factors, including genotype, explant type and plant growth regulators (PGRs) among others.…”

Section: Micropropagation By Somatic Embryogenesismentioning

confidence: 99%

“…Embryogenic callus induction using 100 mg/L 2,4-D was reported in many date palm cultivars such as Khanizi and Mordarsing [11], Khasab and Nabout [12] and Nabout Saif [19]. However, Fki et al mentioned that high doses of 2,4-D may induce somaclonal variation [9]. Therefore, other researchers used lower 2,4-D concentrations or other auxins in order to induce somatic embryogenesis.…”

Section: Embryogenic Callus Induction and Somatic Embryo Formationmentioning

confidence: 99%

“…Ferhi using RAPD primers [70]. Low concentrations of auxins, mainly 2,4-D, low numbers of subcultures and the use of juvenile explants were suggested to decrease the risk of somaclonal variation [9,71,72].…”

Section: Genetic Variability In Regenerantsmentioning

confidence: 99%

“…Propagation of date palm through in vitro techniques presents an efficient alternative for the conventional methods [8]. Indeed, date palm micropropagation allows rapid and large-scale multiplication of uniform and healthy plants, with neither seasonal effects nor the risk of spreading diseases and pests during plant material exchange [9].…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Date Palm: A Review

Mazri¹,

Meziani²

2015

Cell Dev Biol

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Somatic embryogenesis and organogenesis are the two pathways of choice for rapid and large-scale propagation of date palm. They have been successfully used for the micropropagation of elite genotypes and have proved their effectiveness for the commercial production of many cultivars. Nevertheless, regeneration through somatic embryogenesis or organogenesis in date palm is still difficult to achieve for recalcitrant genotypes and is often hampered by certain physiological disorders. In the present review, we report the results of a number of studies carried out on date palm micropropagation. It also describes different factors that influence each stage of somatic embryogenesis and organogenesis and the main problems encountered during these two micropropagation processes.

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Section: Micropropagation By Somatic Embryogenesismentioning

confidence: 99%

Section: Embryogenic Callus Induction and Somatic Embryo Formationmentioning

confidence: 99%

Section: Genetic Variability In Regenerantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Date Palm: A Review

Mazri¹,

Meziani²

2015

Cell Dev Biol

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“…Research on the application of tissue culture techniques for the identification of salt and drought tolerant date palm has been somewhat neglected. This despite the availability of effective in vitro regeneration systems of various date palm cultivars; through both somatic embryogenesis (Fki et al, 2003;Sané et al, 2006;Taha et al, 2007;Zouine and El Hadrami, 2007;Othmani et al, 2009;Al-Khayri, 2010;Fki et al, 2011;Fki et al, 2011;Sané et al, 2012;Al-Khayri, 2013) and organogenesis (Taha et al, 2001;Sudhersan and AboEl-Nil, 2004;Khierallah and Bader, 2007;Abahmane, 2011). Nonetheless, not a single study thus far has reported successful in vitro regeneration of date palm plantlets with enhanced tolerance to abiotic stress factors.…”

Section: Introductionmentioning

confidence: 99%

In vitro selection of abiotic stress tolerant date palm (Phoenix dactylifera L.): A review

Al-Khayri

Ibraheem²

2014

Emir. J. Food Agric

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The advent of agricultural biotechnology offers new approaches for the genetic improvement of date palm (Phoenix dactylifera L.), an important economic fruit crop in arid regions. In vitro studies were conducted to recover date palm plants exhibiting enhanced tolerance to salinity and drought stress, two major agricultural problems in arid areas. The response of date palm callus cultures to salt stress was investigated by exposing cell suspension and callus cultures to varying concentrations of sodium chloride (NaCl). The reaction of callus to the duration of exposure to NaCl, potassium chloride (KCl) and calcium chloride (CaCl2) was evaluated. Similarly, drought tolerance was assessed in response to varying concentrations of polyethylene glycol (PEG-8000). Significant differences were reported in various parameters measured including callus growth, somatic embryogenesis, proline content and ion distribution. Calli with enhanced tolerance to these selection agents were obtained; however, plant regeneration from these cell lines was not realized. Although the available data provide an insight of the behavior of date palm callus in the presence of salt and drought stress, further research work is required to refine the selection procedures and plant regeneration from the selected tolerant cell lines.

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Desiccation and Cold Hardening of Date Palm Somatic Embryos Improve Germination

Shareef

2017

Methods in Molecular Biology

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Embryogenic suspension cultures of date palm are ideal for mass propagation of somatic embryos; however, the low percentage of germination of somatic embryos (SE) remains an impediment. This chapter focuses on two important physical factors to improve germination of date palm somatic embryos: the use of partial desiccation (3 h) of somatic embryos and the exposure to low temperature (4 °C for 24 h). High germination percentage (41%) is achieved by desiccation for 3 h. Moreover, adding 0.3 g/L activated charcoal (AC) to the liquid medium further improves somatic embryo number and weight as well as the percentage of germination. Moreover, partial desiccation and low temperature exposure tend to increase proline content. This improved protocol for somatic embryo germination is potentially applicable for commercial micropropagation of date palm.

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Date Palm Micropropagation via Somatic Embryogenesis

Cited by 44 publications

References 59 publications

Micropropagation of Date Palm: A Review

Micropropagation of Date Palm: A Review

In vitro selection of abiotic stress tolerant date palm (Phoenix dactylifera L.): A review

Desiccation and Cold Hardening of Date Palm Somatic Embryos Improve Germination

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