Mj-DWD, a double WAP domain-containing protein with antiviral relevance in Marsupenaeus japonicus

Sequence diversity and evolution of antimicrobial peptides in invertebrates

“…The putative type IV crustins (DWD-containing proteins) con-778 tain two WAP domains in the mature peptide. To date, type IV 779 crustins have only been found in crustaceans, and specifically 780 one isoform in shrimps (Chen et al, 2008;Du et al, 2009;781 Jiménez-Vega and Vargas-Albores, 2007;Suthianthong et al, 782 2011) and two isoforms in crabs . (Suthianthong et al, 2011).…”

“…Subse-587 quently, based on genomics research, several cDNAs of crustin 588 and crustin-like peptides have been identified from several tissues 589 of crustaceans (Afsal et al, 2011, 2013; Antony et al, 2010, 2011; 590 Arockiaraj et al, 2013; Battison et al, 2008; Brockton and Smith, 591 2008; Cui et al, 2012; Donpudsa et al, 2010; Hauton et al, 2006; 592Imjongjirak et al, 2009;Jiravanichpaisal et al, 2007; Kim et al, 593 2012;Liu et al, 2011;Mu et al, 2010Mu et al, , 2011 Pisuttharachai et al, 594 2009b;Smith, 2011;Smith et al, 2008; Soonthornchai et al, 595 2010;Sperstad et al, 2009Sperstad et al, , 2010Sun et al, 2010; Tassanakajon 596 et al, 2010;Yue et al, 2010) and different species from the insect 597 order Hymenoptera(Zhang and Zhu, 2012).598 Crustins are cationic cysteine-rich AMPs that contain a multi-599 domain region (glycine-rich, proline-rich or cysteine-rich) at the 600 N-terminus and a four-disulfide core (4-DSC) with a whey acidic 601 protein (WAP) domain at the C-terminus(Smith, 2011; Smith 602 et al, 2008;Tassanakajon et al, 2010). Crustins are currently clas-603 sified into three major groups (types I-III), but 604 recently double WAP domain (DWD)-containing proteins have 605 been discovered in crustaceans and found to exhibit antimicrobial 606 activities(Chen et al, 2008;Du et al, 2009; Jiménez-Vega and 607 Vargas-Albores,2007;Li et al, 2012Smith, 2011; 608 Suthianthong et al, 2011), whilst based on comparative genomic 609 analysis, a novel type of insect crustin has been identified in an 610 ant(Zhang and Zhu, 2012). In this review, we include DWD-con-611 taining proteins (types I-III) and also the novel ant crustins as type 612 IV and type V crustins, respectively.…”

Molecular Characterization and Antibacterial Activity Analysis of Two Novel Penaeidin Isoforms from Pacific White Shrimp, Litopenaeus vannamei

“…There were also some reports about AMPs' antiviral activity. Due to some recent results about antiproteinase activity [6,11,13,14,23,24], AMPs' action mechanism involved in the antibacterial process should be reconsidered and given further perfection. Once the action mechanism was thoroughly solved, AMPs could be considered as the prophylactic target to build some strategies to control some diseases in shrimp aquaculture [25].…”

“…To date, four major types of AMPs were identified, including penaeidins, crustins, antilipopolysaccharide factors, and lysozymes [6]. These AMP molecules have been extensively reported in several shrimps in the past decade, for example, Fenneropenaeus chinensis [7], Procambarus clarkii [8], Penaeus monodon [9], Fenneropenaeus indicus [10], Marsupenaeus japonicus [11], and so on. In recent years, there were also some novel results about AMPs' biological activities.…”

A double WAP domain-containing protein PmDWD from the black tiger shrimp Penaeus monodon is involved in the controlling of proteinase activities in lymphoid organ

“…It was only recently that the DWDs had been identified from penaeid shrimp Litopenaeus vannamei [20], Marsupenaeus japonicus [21] and Fenneropenaeus chinensis [22]. They are called double WAP domain proteins or DWD for the whole molecule contains two WAP domains without any other extra motifs unlike those of crustins.…”