Mixotrophic growth regime as a strategy to develop microalgal bioprocess from nutrimental composition of tequila vinasses

Abstract

The selection of a suitable growth regime can increase the physiological performance of microalgae and improve bioprocess based on these microorganisms from agro-industrial residues. Thus, this study assessed the biotechnology capacity-biomass production, biochemical composition, and nutrient uptake-from tequila vinasses (TVs) as the nutrient source of three indigenous microalgae-Chlorella sp., Scenedesmus sp., and Chlamydomonas sp.-cultured under heterotrophic and mixotrophic conditions. The results demonstrated that under the mixotrophic regime, the three microalgae evaluated reached the highest nitrogen uptake, biomass production, and cell compound accumulation. Under this condition, Chlorella sp. and Scenedesmus sp. showed the highest nutrient uptake and biomass production, 1.7 ± 0.3 and 1.9 ± 0.3 g L −1 , respectively; however, the biochemical composition, mainly carbohydrates and proteins, varied depending on the microalgal strain and its growth regime. Overall, our results demonstrated the biotechnological capacity of native microalgae from TVs, which may vary not only depending on the microalgal strain but also the culture strategy implemented and the characteristics of the residue used, highlighting-from a perspective of circular bio-economy-the feasibility of implementing microalgal bioprocess to reuse and valorize the nutrimental composition of TVs through biomass and high-valuable metabolite production, depicting a sustainable strategy for tequila agro-industry in Mexico.

Untitled

“…ATP yang terbentuk dari reaksi fotokimia seharusnya mempercepat anabolisme dari sumber karbon organik pada kultur miksotrofik T. chui dan ini menjadi alasan untuk terjadinya peningkatan pertumbuhan pada kultur tersebut. Choix et al, (2021) menunjukkan bahwa kultur pada kondisi heterotrofik dan fotoautotrofik memiliki laju pertumbuhan sel yang lebih rendah dibandingkan pada kondisi miksotrofik. Pada kajian ini terlihat jelas bahwa T. chui berasimilasi dan tumbuh dengan adanya sumber karbon anorganik (CO2) dan organik (hidrolisat talas) dengan hadirnya cahaya (kondisi miksotrofik).…”

Current Panorama, Practice Gaps, and Recommendations to Accelerate the Transition to a Circular Bioeconomy in Latin America and the Caribbean

“…Some of the practices and initiatives for a CBE in LAC countries can be found in the existing literature, and the majority of them are reported in case studies. Waste seems to be the main starting point for CBE initiatives in LA countries [e.g., 13, 29, 97. Bioenergy production seems recurrent [20,87], mainly based on the use of waste-derived biogas, and consequently the production of biofertilizer, as well as the production of biodiesel [20].…”