Mixing behaviour of asymmetrical glycerol diether bolalipids with saturated and unsaturated phosphatidylcholines

Müller, Sindy; Meister, Annette; Otto, Christian F.; Hause, Gerd; Drescher, Simon

doi:10.1016/j.bpc.2018.04.005

Cited by 5 publications

(3 citation statements)

References 55 publications

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“…During this project, two novel classes of artificial bolalipids, the glycerol diether bolalipids [160,161] and alkyl-substituted single-chain bolalipids [162,163], were synthesized and characterized. Drescher and his group could show that these artificial bolalipids are miscible with unsaturated phospholipids [164,165], and that these mixed vesicles (bolasomes) show promising results regarding the stability of those bolasomes in artificial digestive media [166]. However, these bolalipid-doped liposomes require further investigation before they can be used as a drug delivery system.…”

Section: Principalmentioning

confidence: 99%

The Phospholipid Research Center: Current Research in Phospholipids and Their Use in Drug Delivery

Drescher

Hoogevest

2020

Pharmaceutics

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This review summarizes the research on phospholipids and their use for drug delivery related to the Phospholipid Research Center Heidelberg (PRC). The focus is on projects that have been approved by the PRC since 2017 and are currently still ongoing or have recently been completed. The different projects cover all facets of phospholipid research, from basic to applied research, including the use of phospholipids in different administration forms such as liposomes, mixed micelles, emulsions, and extrudates, up to industrial application-oriented research. These projects also include all routes of administration, namely parenteral, oral, and topical. With this review we would like to highlight possible future research directions, including a short introduction into the world of phospholipids.

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Section: Principalmentioning

confidence: 99%

The Phospholipid Research Center: Current Research in Phospholipids and Their Use in Drug Delivery

Drescher

Hoogevest

2020

Pharmaceutics

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“…As published previously [53,56], DSC measurements were carried out using a MicroCal VP-DSC differential scanning calorimeter (MicroCal Inc. Northampton, MA, USA). Before each measurement, the lipid dispersion (c = 3 mM) and the reference were degassed under vacuum while stirring.…”

Section: Differential Scanning Calorimetry (Dsc)mentioning

confidence: 99%

“…In accordance to a procedure described previously [56], vitrified specimens for cryo-TEM were prepared by a blotting procedure using an EM GP grid plunger (Leica, Wetzlar, Germany), and an atmospheric chamber with controlled humidity (80%) and temperature (T = 22 • C). For cryo-fixation, a drop of the sample solution (c = 3 mM) was placed onto an EM grid that is coated with a holey carbon film (C-flat, Protochip Inc., Raleight, NC, USA).…”

Section: Transmission Electron Microscopy Of Vitrified Specimens (Crymentioning

confidence: 99%

Bolalipid-Doped Liposomes: Can Bolalipids Increase the Integrity of Liposomes Exposed to Gastrointestinal Fluids?

Müller

Gruhle

Meister³

et al. 2019

Pharmaceutics

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The use of archaeal lipids and their artificial analogues, also known as bolalipids, represents a promising approach for the stabilization of classical lipid vesicles for oral application. In a previous study, we investigated the mixing behavior of three single-chain alkyl-branched bolalipids PC-C32(1,32Cn)-PC (n = 3, 6, 9) with either saturated or unsaturated phosphatidyl-cholines. We proved, that the bolalipids PC-C32(1,32C6)-PC and PC-C32(1,32C9)-PC show miscibility with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). In the present work, we extended our vesicle system to natural lipid mixtures using phosphatidylcholine from soy beans, and we investigated the effect of incorporated bolalipids on the integrity of these mixed liposomes (bolasomes) in different gastrointestinal fluids using a dithionite assay and a calcein release assay in combination with particle size measurements. Finally, we also studied the retention of calcein within the bolasomes during freeze-drying. As a main result, we could show that in particular PC-C32(1,32C6)-PC is able to increase the stability of bolasomes in simulated gastric fluid—a prerequisite for the further use of liposomes as oral drug delivery vehicles.

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Mixing behaviour of bilayer-forming phosphatidylcholines with single-chain alkyl-branched bolalipids: effect of lateral chain length

Müller

Kind

Gruhle

et al. 2019

Biophysical Chemistry

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Mixing behaviour of asymmetrical glycerol diether bolalipids with saturated and unsaturated phosphatidylcholines

Cited by 5 publications

References 55 publications

The Phospholipid Research Center: Current Research in Phospholipids and Their Use in Drug Delivery

The Phospholipid Research Center: Current Research in Phospholipids and Their Use in Drug Delivery

Bolalipid-Doped Liposomes: Can Bolalipids Increase the Integrity of Liposomes Exposed to Gastrointestinal Fluids?

Mixing behaviour of bilayer-forming phosphatidylcholines with single-chain alkyl-branched bolalipids: effect of lateral chain length

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