Mixed species biofilms of Fusobacterium necrophorum and Porphyromonas levii impair the oxidative response of bovine neutrophils in vitro

Lockhart, J. Scott; Buret, André G.; Storey, Douglas G.; Anderson, Stefanie Judith; Morck, Douglas W.

doi:10.1016/j.anaerobe.2017.05.008

Cited by 9 publications

(9 citation statements)

References 47 publications

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“…can help identify whether or not the species have unique roles in lesions. Given that F. necrophorum has been shown to generate mixed-species biofilms with P. levii to impair the neutrophil response ( 29 ), it is possible that the Fusobacterium sp. that we isolated in this study may interact in a similar way.…”

Section: Discussionmentioning

confidence: 99%

Identification and Quantification of Bovine Digital Dermatitis-Associated Microbiota across Lesion Stages in Feedlot Beef Cattle

et al. 2021

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Section: Discussionmentioning

confidence: 99%

Identification and Quantification of Bovine Digital Dermatitis-Associated Microbiota across Lesion Stages in Feedlot Beef Cattle

et al. 2021

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“…However, in periodontal disease, which has similar higher level microbial community structure to DD, Porphyromonas plays a significant role in influencing the metabolic activity of Treponema species (Tan et al, 2014). Additionally, Porphyromonas and Fusobacterium species generate mixed-species biofilms as a mechanism to impair the bovine innate immune system (Lockhart et al, 2017). While it is not evident that the same microbial strategies are involved in DD pathogenesis, it provides significant motivation to identify interactions between DDassociated species to determine a framework of the synergistic mechanisms used as pathogenesis mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Meta-Analysis of Bovine Digital Dermatitis Microbiota Reveals Distinct Microbial Community Structures Associated With Lesions

Caddey

Buck

2021

Front. Cell. Infect. Microbiol.

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Bovine digital dermatitis (DD) is a significant cause of infectious lameness and economic losses in cattle production across the world. There is a lack of a consensus across different 16S metagenomic studies on DD-associated bacteria that may be potential pathogens of the disease. The goal of this meta-analysis was to identify a consistent group of DD-associated bacteria in individual DD lesions across studies, regardless of experimental design choices including sample collection and preparation, hypervariable region sequenced, and sequencing platform. A total of 6 studies were included in this meta-analysis. Raw sequences and metadata were identified on the NCBI sequence read archive and European nucleotide archive. Bacterial community structures were investigated between normal skin and DD skin samples. Random forest models were generated to classify DD status based on microbial composition, and to identify taxa that best differentiate DD status. Among all samples, members of Treponema, Mycoplasma, Porphyromonas, and Fusobacterium were consistently identified in the majority of DD lesions, and were the best genera at differentiating DD lesions from normal skin. Individual study and 16S hypervariable region sequenced had significant influence on final DD lesion microbial composition (P < 0.05). These findings indicate that members of Treponema, Mycoplasma, Porphyromonas, and/or Fusobacterium may have significant roles in DD pathogenesis, and should be studied further in respect to elucidating DD etiopathogenic mechanisms and developing more effective treatment and mitigation strategies.

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“…In chronic wounds, the local environment is associated with low oxygen levels that enable the growth of these bacteria [40]. In addition, Fusobacteria and Porphyromonas are commonly found in combination and, in a laboratory setting, have been shown to form biofilm and could therefore impair the healing of wounds [45]. In line with this, the severe sample (S7) that was re-sampled after a wound duration of around three months showed an increased proportion of these two bacteria.…”

Section: Plos Onementioning

confidence: 84%

A shotgun metagenomic investigation of the microbiota of udder cleft dermatitis in comparison to healthy skin in dairy cows

et al. 2020

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Udder cleft dermatitis (UCD) is a skin condition affecting the fore udder attachment of dairy cows. UCD may be defined as mild (eczematous skin changes) or severe (open wounds, large skin changes). Our aims were to compare the microbiota of mild and severe UCD lesions with the microbiota of healthy skin from the fore udder attachment of control cows, and to investigate whether mastitis-causing pathogens are present in UCD lesions. Samples were obtained from cows in six dairy herds. In total, 36 UCD samples categorized as mild (n = 17) or severe (n = 19) and 13 control samples were sequenced using a shotgun metagenomic approach and the reads were taxonomically classified based on their k-mer content. The Wilcoxon rank sum test was used to compare the abundance of different taxa between different sample types, as well as to compare the bacterial diversity between samples. A high proportion of bacteria was seen in all samples. Control samples had a higher proportion of archaeal reads, whereas most samples had low proportions of fungi, protozoa and viruses. The bacterial microbiota differed between controls and mild and severe UCD samples in both composition and diversity. Subgroups of UCD samples were visible, characterized by increased proportion of one or a few bacterial genera or species, e.g. Corynebacterium, Staphylococcus, Brevibacterium luteolum, Trueperella pyogenes and Fusobacterium necrophorum. Bifidobacterium spp. were more common in controls compared to UCD samples. The bacterial diversity was higher in controls compared to UCD samples. Bacteria commonly associated with mastitis were uncommon. In conclusion, a dysbiosis of the microbiota of mild and severe UCD samples was seen, characterized by decreased diversity and an increased proportion of certain bacteria. There was no evidence of a specific pathogen causing UCD or that UCD lesions are important reservoirs for mastitis-causing bacteria.

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Mixed species biofilms of Fusobacterium necrophorum and Porphyromonas levii impair the oxidative response of bovine neutrophils in vitro

Cited by 9 publications

References 47 publications

Identification and Quantification of Bovine Digital Dermatitis-Associated Microbiota across Lesion Stages in Feedlot Beef Cattle

Identification and Quantification of Bovine Digital Dermatitis-Associated Microbiota across Lesion Stages in Feedlot Beef Cattle

Meta-Analysis of Bovine Digital Dermatitis Microbiota Reveals Distinct Microbial Community Structures Associated With Lesions

A shotgun metagenomic investigation of the microbiota of udder cleft dermatitis in comparison to healthy skin in dairy cows

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