Mixed metal oxide nanoparticles inhibit growth of Mycobacterium tuberculosis into THP-1 cells

Jafari, Alireza; Mosavi, Tahereh; Mosavari, Nader; Majid, Amin Malik Shah Abdul; Movahedzade, F.; Tebyaniyan, M.; Kamalzadeh, Morteza; Dehgan, M.; Jafari, Sahar; Arastoo, Shahrdad

doi:10.1016/j.ijmyco.2016.09.011

Cited by 28 publications

(11 citation statements)

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“…The toxicity of the QDs in THP-1 leukemic cells was also studied. Results showed that the 8ZnO/2Ag ratio was the most effective since it had high antibacterial capacity against Mtb both in vivo and in vitro but without affecting the cellular viability of THP-1 cells [ 81 ]. In 2016, R. Jalal et al used different techniques (fluorescence and scanning electron microscopy, flow cytometry and DNA extraction) to elucidate the mechanism of action of ZnO QDs administered alone and in combination with two antibiotics ciprofloxacin and ceftazidime.…”

Section: Zno Nanoplatforms For Bacterial Infectionmentioning

confidence: 99%

ZnO Nanostructures for Drug Delivery and Theranostic Applications

2018

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In the last two decades, zinc oxide (ZnO) semiconductor Quantum dots (QDs) have been shown to have fantastic luminescent properties, which together with their low-cost, low-toxicity and biocompatibility have turned these nanomaterials into one of the main candidates for bio-imaging. The discovery of other desirable traits such as their ability to produce destructive reactive oxygen species (ROS), high catalytic efficiency, strong adsorption capability and high isoelectric point, also make them promising nanomaterials for therapeutic and diagnostic functions. Herein, we review the recent progress on the use of ZnO based nanoplatforms in drug delivery and theranostic in several diseases such as bacterial infection and cancer.

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Section: Zno Nanoplatforms For Bacterial Infectionmentioning

confidence: 99%

ZnO Nanostructures for Drug Delivery and Theranostic Applications

2018

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“…On the other hand, using just silver nanoparticles may be ineffective against some bacteria, because the antibacterial properties of silver nanoparticles are weak. Based on previous studies, synergistic effects of silver and zinc oxide nanoparticles (AgZnONPs) have been proven effective on various bacteria . Zinc oxides has been recognized as antibacterial material in recent years.…”

Section: Introductionmentioning

confidence: 99%

Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage

et al. 2017

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The World Health Organization acknowledges tuberculosis as a global threat.Tuberculosis infection is one of the top 10 causes of death worldwide. Nanotechnology and microbiology researchers are looking for new and safe nano drugs for eliminating Mycobacterium tuberculosis, the causative agent of tuberculosis. In this study, AgZnO nano-crystals (AgZnONCs) is synthesized via the decomposition of the precursor of oxalate method. Characterization of AgZnONCs were evaluated. Next, various concentrations of AgZnONCs, as well AgZnONCs+Rifampicin, were prepared. The MTT assay was employed to study the viability of human macrophage cell lines (THP-1) exposed to AgZnONCs. The bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were studied by Minimum Bactericidal Concentration (MBC) test. Subsequently, THP-1 were infected by H 37 Rv strain of M. tuberculosis (H 37 RvMtb). Also, bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were compared with ex-vivo conditions. The MBC of AgZnONCs and AgZnONCs+Rifampicin were ratios of 1:4 and 1:32 respectively (p-value <0.05). Also, more than 50% and 80% of THP-1 were alive in ratios of 1:4 and 1:32 in the presence of AgZnONCs, respectively. All phagocytic H 37 RvMtb were killed in the presence of AgZnONCs+Rifampicin (p-value <0.05),

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“…In 2016, studies have suggested the ability of Ag-ZnO NPs to inhibit the growth of the standard laboratory strain of M. tuberculosis H37Rv 32. However, there is no sufficient data regarding the antibacterial effects of Ag, ZnO, and Ag-ZnO NPs against the drug-resistant clinical strains of M. tuberculosis .…”

Section: Discussionmentioning

confidence: 99%

<p>The Anti-Mycobacterial Activity Of Ag, ZnO, And Ag- ZnO Nanoparticles Against MDR- And XDR-<em>Mycobacterium tuberculosis</em></p>

Heidary¹,

Bostanabad²,

Amini³

et al. 2019

IDR

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BackgroundNowadays, tuberculosis (TB) is one of the top ten leading causes of mortality worldwide. The emergence of multidrug-resistant (MDR) – and extensively drug-resistant (XDR) – Mycobacterium tuberculosis (M. tuberculosis) is identified as one of the most challenging threats to TB control. Thus, new and safe nano-drugs are urgently required for the elimination of TB. The aim of this study was to investigate the anti-bacterial effects of Ag, ZnO, and Ag-ZnO nanoparticles (NPs) on MDR- and XDR-M. tuberculosis.Materials and methodsIn this study, Ag, ZnO, and Ag-ZnO NPs were synthesized by the chemical reduction and chemical deposition methods. NPs were characterized using ultraviolet–visible spectroscopy, dynamic light scattering, and transmission electron microscopy. Then, various dilutions of NPs were prepared and their minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined against M. tuberculosis strains using the broth microdilution and agar microdilution methods. Finally, MTT test and cell culture assay were performed.ResultsThe effects of concentrations of 1–128 µg/mL Ag NPs, ZnO NPs, 2Ag: 8ZnO, 8Ag:2ZnO, 3Ag: 7ZnO, 7Ag:3ZnO, and 5Ag:5ZnO on M. tuberculosis strains were investigated. MIC results showed the inhibitory effect of 1 µg/mL of all NPs against XDR-M. tuberculosis. In addition, the concentrations of 4 µg/mL Ag, 8 µg/mL 5Ag:5ZnO, 8 µg/mL 7Ag:3ZnO, 32 µg/mL 3Ag:7ZnO, 16 µg/mL 8Ag:2ZnO, and 64 µg/mL 2Ag:8ZnO inhibited MDR-M. tuberculosis growth. However, MBC results indicated the inability of Ag, ZnO and Ag-ZnO NPs, either in combination or alone, to kill MDR- or XDR-M. tuberculosis.ConclusionTo the best of our knowledge, this is the first study to evaluate the effects of Ag and ZnO NPs against MDR and XDR strains of M. tuberculosis. According to the results, Ag and ZnO NPs showed bacteriostatic effects against drug-resistant strains of M. tuberculosis. Therefore, these NPs may be considered as promising anti-mycobacterial nano-drugs. However, further studies are required to affirm the bactericidal effects of these NPs against TB.

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Mixed metal oxide nanoparticles inhibit growth of Mycobacterium tuberculosis into THP-1 cells

Cited by 28 publications

References 0 publications

ZnO Nanostructures for Drug Delivery and Theranostic Applications

ZnO Nanostructures for Drug Delivery and Theranostic Applications

Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage

<p>The Anti-Mycobacterial Activity Of Ag, ZnO, And Ag- ZnO Nanoparticles Against MDR- And XDR-<em>Mycobacterium tuberculosis</em></p>

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