Mixed culture polyhydroxyalkanoate-rich biomass assessment and quality control using thermogravimetric measurement methods

Chan, Clement Matthew; Johansson, Peter; Magnusson, Per; Vandi, Luigi‐Jules; Arcos-Hernández, Mónica V.; Halley, P. J.; Laycock, Bronwyn; Pratt, Steven; Werker, Alan

doi:10.1016/j.polymdegradstab.2017.07.029

Cited by 40 publications

(23 citation statements)

References 22 publications

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“…VFAs were analyzed by gas chromatography (GC) as previously reported [11]. Biomass PHA content was evaluated by thermogravimetric analysis (Q500 TA Instruments) as previously described [21,22] and PHA composition was assessed by GC [23]. Total suspended solids (TSS) and volatile suspended solids (VSS) measurements were made according to standard methods [24].…”

Section: Methodsmentioning

confidence: 99%

Influence of temperature on mixed microbial culture polyhydroxyalkanoate production while treating a starch industry wastewater

Grazia

Quadri

Majone

et al. 2017

Journal of Environmental Chemical Engineering

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Influence of temperature on mixed microbial culture polyhydroxyalkanoate production while treating a starch industry wastewater, Journal of Environmental Chemical Engineeringhttp://dx.Engineering (Brisbane, Queensland, Australia); 4 Promiko AB (Lomma, Sweden).Corresponding author: Alan Werker (alan@werker.se) Graphical abstractHighlights  Enrichment biomass at 15 and 25 °C gave similar PHA accumulation potential  PHA accumulation process robust to temperatures from 15 to 30°C  Kinetics change with temperature according to modified Arrhenius equation  Mixed culture PHA production is more efficient at lower temperatures  Bioprocess is adaptable to anticipated seasonal water temperature changes AbstractThe influence of temperature on the production of enrichment biomass and polyhydroxyalkanoates by activated sludge was evaluated within a practical case study. Two laboratory-scale sequencing batch reactors (SBRs) were operated in parallel over 131 days to treat a wastewater from a potatostarch modification facility, and produce surplus activated sludge biomass with PHA accumulation potential. The SBRs were operated similarly but at different temperatures (15 and 25°C).Temperature did not influence wastewater treatment performance (average 97% COD removal).Replicate PHA accumulation experiments were conducted on the SBR surplus biomass at 15, 20, 25 and 30°C. Surplus biomass accumulated PHA with acetic acid to between 60 and 65%, gPHA/gVSS for all temperatures tested and with estimated Arrhenius temperature coefficients (θ) of 1.048 for the PHA production, and 1.062 for the COD consumption specific rates. The MMC PHA production process was adaptable within a common range anticipated for seasonal fluctuation of temperature for the influent wastewater and PHA production feedstocks without loss of performance. This outcome suggests a broad range in the practical feasibility and reliability with MMC method and process implementations. PHA production was predicted to be more efficient at lower temperatures. Strategies can be engineered in the bioprocess design and operations to accommodate for temperature shifts in practical applications of PHA production as a route to resource recovery from organic waste management services.

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Section: Methodsmentioning

confidence: 99%

Influence of temperature on mixed microbial culture polyhydroxyalkanoate production while treating a starch industry wastewater

Grazia

Quadri

Majone

et al. 2017

Journal of Environmental Chemical Engineering

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“…Surplus activated sludge biomass was harvested from a pilot-scale biological process treating a potato starch manufacturing factory wastewater (AKM, Denmark). The biomass was used to accumulate PHA using a pilot scale aerobic fed-batch reactor and processing which has been described in full in a previous study [Chan et al, 2017a;Morgan-Sagastume et al, 2014].…”

Section: Mixed Culture Phbv Accumulationmentioning

confidence: 99%

“…The dried biomass was then sent to a pilot-scale extraction process whereby the PHBV was extracted with butanol to a maximum temperature 140 ○ C over 53 min [Chan et al, 2017a]. The solvent containing dissolved polymer was then separated from the biomass suspended solids by filtering and the solvent mix was cooled to form a gel, then pressed to exude excess solvent.…”

Section: Mixed Culture Phbv Accumulationmentioning

confidence: 99%

“…Optimisation of the post-processing steps could mitigate the adverse effect [Chan et al, 2017a]. The P3HB4HB furthermore exhibited residual mass after PHA decomposition, suggesting a higher level of impurity.…”

Section: Thermal Stabilitymentioning

confidence: 99%

“…average scission rates (RS) were also estimated using the following equations [Chan et al, 2017a] and are presented in Table 7-5:…”

Section: Thermal Stabilitymentioning

confidence: 99%

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Development of bio-derived and biodegradable polyhydroxyalkanoate (PHA)-based wood plastic composites (WPCs)

Chan¹

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In response to the concerns over the accumulation of plastic wastes, the wood plastic composites (WPCs) industry has been in particular interest in replacing the petroleum-based polymer matrices with biopolymers. Polyhydroxyalkanoates (PHAs) are potential candidates as these biopolymers can be degraded readily under ambient conditions and can be synthesised intracellularly by bacteria from renewable/waste resources. Their low melt viscosity can also be an advantage in processing. While there is a considerable body of literature on PHA-based WPCs, the majority of the studies have focused on mechanical property optimisation but their mechanical stability and biodegradability were poorly understood. Therefore, this thesis explored the fundamental characteristics of PHA/wood flour (WF) composites with focus on the micro-structure-property relationship and end-of-life behaviour. The extrusion processing setup for composites of a commercially available poly(3-hydroxybutyrateco-3-hydroxyvalerate) (PHBV) and WF was first optimised. A logical next step involved the optimisation of mechanical properties through the use of carefully selected chemical compatibilisers and additives. The expected benefits of chemical compatibilisers were outweighed when compared to the alteration of micro-structure by the non-reactive micro-sized talc filler. An innovative approach for improving processabilty and toughness was then investigated through the inclusion of tougher PHA copolymers including an in-house mixed culture PHBV, for further cost reduction. The potential of this strategy was demonstrated by the improvement, albeit marginal, in composite strain at break. The real-time studies of mechanical stability and biodegradability brought new insights to this biocomposite. Uncoated PHBV/WF composites were stable under indoor ambient conditions for at least 1 year. Exposure to outdoor environment, however, led to reduced mechanical stability. It was determined to be solely associated with the mould growth on the exposed hygroscopic wood particles. The unique advantage of PHBV/WF composites was demonstrated with respect to their significantly higher biodegradation rate in soil than PLA/WF and PE/WF composites. The presence of wood accelerated the biodegradation and reduced the mechanical stability of the composites in soil. A micro-mechanical schematic model was used to illustrate the phenomena. In addition, it was shown that PHA-based WPCs are physically and mechanically stable unless they are exposed to colonies of microorganisms such as moisture-induced mould or those found in soil communities. Overall, this thesis provided better insights into the strong correlations between the micro-structure and the properties of PHA-based WPCs, both initially and in the long-term. Wood particles played an important role in both their performance and biodegradation. To conclude, this thesis has paved the way for development of novel bio-derived and biodegradable composites which provide utility what would otherwise be a low-value stream from the f...

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Polyhydroxyalkanoates (PHAs) – Production, Properties, and Biodegradation

Koller

Mukherjee²

2022

Biodegradable Polymers in the Circular Plastics Economy

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Mixed culture polyhydroxyalkanoate-rich biomass assessment and quality control using thermogravimetric measurement methods

Cited by 40 publications

References 22 publications

Influence of temperature on mixed microbial culture polyhydroxyalkanoate production while treating a starch industry wastewater

Influence of temperature on mixed microbial culture polyhydroxyalkanoate production while treating a starch industry wastewater

Development of bio-derived and biodegradable polyhydroxyalkanoate (PHA)-based wood plastic composites (WPCs)

Polyhydroxyalkanoates (PHAs) – Production, Properties, and Biodegradation

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