Polyhydroxyalkanoates (<scp>PHAs</scp>) – Production, Properties, and Biodegradation

Koller, Martin; Mukherjee, Anindya

doi:10.1002/9783527827589.ch6

Cited by 8 publications

(8 citation statements)

References 181 publications

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“…The extraction process is a major cost factor besides feedstock cost for PHA production. Additionally, downstream processing has a large ecological footprint due to using chemicals, solvents, and energy [10]. In the past few years, several ongoing studies on PHA recovery aimed to reduce or substitute the use of chemicals and solvents to decrease the ecological footprint and maximize PHA yields [27].…”

Section: Downstream Processing and Economic Advantagesmentioning

confidence: 99%

“…Several companies have already adopted PHA production on a large scale using aerobic bacteria. However, high production costs are still significant, so commercialization is still not widespread [9,10]. Major expenses in the production of PHA are determined by the fermentation substrate's cost and the polymer's extraction from inside the cell [11].…”

Section: Introductionmentioning

confidence: 99%

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Using inorganic acids to stop purple phototrophic bacteria metabolism improves PHA recovery at a large scale

Srivastava

Villamil

Melero

et al. 2023

Biomass Conv. Bioref.

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Polyhydroxyalkanoate (PHA) production at a large scale by purple phototrophic bacteria (PPB) is hindered due to high production costs and limited recovery due to its consumption during starvation periods. The present study identified cost-effective inorganic acids as inactivation methods for PPB to obtain higher PHA recovery. The study was performed on reactors of different scales (10 L and 0.5 L) to grow PPB and recover PHA subsequently. The permanent feast strategy was adopted to obtain higher PHA in an anaerobic environment. As a result, the study achieved 33% (dry weight) PHA recovery using inorganic acid inactivation, while formaldehyde inactivation (traditional method) achieved significantly lower PHA recovery (20% only). The results from inorganic acid inactivation were further examined for their stability. The samples were stable even after day 14, and the PHA recovery was the same as on day 0. This pioneering study shows that inorganic acids can be used to inactivate the PPB metabolism to obtain higher PHA recovery; inorganic acid inactivation could be economical for large-scale PHA production. Graphical Abstract

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Section: Downstream Processing and Economic Advantagesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Using inorganic acids to stop purple phototrophic bacteria metabolism improves PHA recovery at a large scale

Srivastava

Villamil

Melero

et al. 2023

Biomass Conv. Bioref.

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“…The different known chemical structures of PHAs differ by the number of carbon atoms of the constituting monomer, and can be classified as short-chain (3–5 carbon atoms) or medium-chain (6–14 carbon atoms) PAHs [ 27 ]. The most common polymers belonging to this family are poly(3-hydroxybutyrate) and poly(4-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), poly(3-hydroxybutyrate-co-4-hydroxybutyrate), and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate).…”

Section: Bioplastics: Definitions and Classificationmentioning

confidence: 99%

“…PHB and PHBV were found to be broken down by depolymerases and hydrolases to 3-hydroxybutyric acid and both 3-hydroxybutyric acid and 3-hydroxyvaleric acid, respectively [ 27 ].…”

Section: Bioplastics Biodegradationmentioning

confidence: 99%

Anaerobic Biodegradability of Commercial Bioplastic Products: Systematic Bibliographic Analysis and Critical Assessment of the Latest Advances

et al. 2023

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Bioplastics have entered everyday life as a potential sustainable substitute for commodity plastics. However, still further progress should be made to clarify their degradation behavior under controlled and uncontrolled conditions. The wide array of biopolymers and commercial blends available make predicting the biodegradation degree and kinetics quite a complex issue that requires specific knowledge of the multiple factors affecting the degradation process. This paper summarizes the main scientific literature on anaerobic digestion of biodegradable plastics through a general bibliographic analysis and a more detailed discussion of specific results from relevant experimental studies. The critical analysis of literature data initially included 275 scientific references, which were then screened for duplication/pertinence/relevance. The screened references were analyzed to derive some general features of the research profile, trends, and evolution in the field of anaerobic biodegradation of bioplastics. The second stage of the analysis involved extracting detailed results about bioplastic degradability under anaerobic conditions by screening analytical and performance data on biodegradation performance for different types of bioplastic products and different anaerobic biodegradation conditions, with a particular emphasis on the most recent data. A critical overview of existing biopolymers is presented, along with their properties and degradation mechanisms and the operating parameters influencing/enhancing the degradation process under anaerobic conditions.

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“…It is acknowledged that there has been a growing need for bioplastics and biodegradable polymers in numerous fields owing to their diversified merits [ 1 ]. Polyesters are in high demanded in several sectors, such as pharmaceutical nanodelivery, food safety, and biomedical applications [ 2 , 3 ]. PHAs are efficient substitutes for petrochemical plastics from fossil fuels.…”

Section: Introductionmentioning

confidence: 99%

The Hyperproduction of Polyhydroxybutyrate Using Bacillus mycoides ICRI89 through Enzymatic Hydrolysis of Affordable Cardboard

2022

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Bioplastics are contemplated as remarkable substitutes for conventional plastics to accommodate green technological advancements. However, their industrial production has not been fully implemented owing to the cost of carbon resources. From another perspective, valorizing different paper mill wastes has become a prominent research topic. These materials may serve as an affording sustainable feedstock for bioplastic production. Adjustment of cardboard waste hydrolysate as suitable fermentation media for production of bacterial polyhydroxyalkanoates (PHAs) has been investigated. Cardboard samples were defibered and dried before enzymatic hydrolysis. The enzymatic degradation of commercial cellulase was monitored over 15 days. Interestingly, 18.2 ± 0.2 g/L glucose yield was obtained from 50 g cardboard samples using a 1.5% (v/v) enzyme concentration. The samples exhibited maximum weight loss values of 69–73%. Meanwhile, five soil samples were collected from local sites in Lodz, Poland. A total of 31 bacterial isolates were screened and cultured on Nile blue plates. Analysis of the 16S rRNA gene sequence of the most potent producer revealed 100% similarity to Bacillus mycoides. Cardboard hydrolysates whole medium, modified MSM with cardboard hydrolysate and nitrogen depleted MSM with cardboard hydrolysate were utilized for PHA production, followed by PHA productivity and cell dry weight (CDW) estimation compared to glucose as a standard carbon source. An impressive PHA accumulation of 56% CDW was attained when the waste hydrolysate was used as a carbon source. FTIR and NMR analysis of the isolated PHA indicated that functional groups of the polymer were related to PHB (polyhydroxybutyrate). Thermal analysis demonstrates that PHB and PHB-CB (PHB produced from cardboard hydrolysate) have degradation temperatures of 380 and 369 °C, respectively, which reflect the high thermal stability and heat resistance compared to the same properties for a standard polymer. This is the first demonstration of full saccharification of corrugated cardboard paper waste for high-level production of PHA. In addition, the attained PHB productivity is one of the highest levels achieved from a real lignocellulosic waste.

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Polyhydroxyalkanoates (PHAs) – Production, Properties, and Biodegradation

Cited by 8 publications

References 181 publications

Using inorganic acids to stop purple phototrophic bacteria metabolism improves PHA recovery at a large scale

Using inorganic acids to stop purple phototrophic bacteria metabolism improves PHA recovery at a large scale

Anaerobic Biodegradability of Commercial Bioplastic Products: Systematic Bibliographic Analysis and Critical Assessment of the Latest Advances

The Hyperproduction of Polyhydroxybutyrate Using Bacillus mycoides ICRI89 through Enzymatic Hydrolysis of Affordable Cardboard

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