Mitotic karyotype analysis in the<i>Vigna</i>genus by means of an image analyser

Venora, Gianfranco; Saccardo, F.

doi:10.1080/00087114.1993.10797255

Cited by 7 publications

(7 citation statements)

References 11 publications

(5 reference statements)

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“…The current TCLs of the Vigna cultivated species were similar to those reported by Shamurailatpam et al . () but considerably different from those described in several other reports (in which the TCLs of Vigna species were <20 μm; Venora & Saccardo ; Venora et al . ; de A Bortoleti et al .…”

Section: Discussioncontrasting

confidence: 92%

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Molecular cytogenetic characterisation and phylogenetic analysis of the seven cultivated Vigna species (Fabaceae)

She

XiangHui

et al. 2014

Plant Biol J

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The genomic organisation of the seven cultivated Vigna species, V. unguiculata, V. subterranea, V. angularis, V. umbellata, V. radiata, V. mungo and V. aconitifolia, was determined using sequential combined PI and DAPI (CPD) staining and dual-colour fluorescence in situ hybridisation (FISH) with 5S and 45S rDNA probes. For phylogenetic analyses, comparative genomic in situ hybridisation (cGISH) onto somatic chromosomes and sequence analysis of the internal transcribed spacer (ITS) of 45S rDNA were used. Quantitative karyotypes were established using chromosome measurements, fluorochrome bands and rDNA FISH signals. All species had symmetrical karyotypes composed of only metacentric or metacentric and submetacentric chromosomes. Distinct heterochromatin differentiation was revealed by CPD staining and DAPI counterstaining after FISH. The rDNA sites among all species differed in their number, location and size. cGISH of V. umbellata genomic DNA to the chromosomes of all species produced strong signals in all centromeric regions of V. umbellata and V. angularis, weak signals in all pericentromeric regions of V. aconitifolia, and CPD-banded proximal regions of V. mungo var. mungo. Molecular phylogenetic trees showed that V. angularis and V. umbellata were the closest relatives, and V. mungo and V. aconitifolia were relatively closely related; these species formed a group that was separated from another group comprising V. radiata, V. unguiculata ssp. sesquipedalis and V. subterranea. This result was consistent with the phylogenetic relationships inferred from the heterochromatin and cGISH patterns; thus, fluorochrome banding and cGISH are efficient tools for the phylogenetic analysis of Vigna species.

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Section: Discussioncontrasting

confidence: 92%

“…Cytogenetic studies on cultivated and wild Vigna species have primarily been restricted to karyomorphological descriptions ( e.g . Sen & Bhowal ; Frahm‐Leliveld ; Joseph & Bouwkamp ; Li ; Rao & Chandel ; Venora & Saccardo ; Venora et al . ; Chen ; Osuji & Edeoga ; Shamurailatpam et al .…”

Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic characterisation and phylogenetic analysis of the seven cultivated Vigna species (Fabaceae)

She

XiangHui

et al. 2014

Plant Biol J

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“…Karyomorphometric analyses of the four I, Tgna species were partially published in Venora & Saccardo (1993). The four species revealed rather symmetrical karyotypes, thus fitting the first four of the 12 categories Stebbins (1971) used to describe karyotype asymmetry.…”

Section: Resultsmentioning

confidence: 99%

“…Karyotype morphometry was made possible by the use of an automated system of image analysis IBAS 2000 by Kontron-Zeiss, which allowed to measure chromosomal indices with a level of precision that cannot be achieved with manual systems (Venora et al, 1991). Slides were prepared and analysed according to Venora & Saccardo (1993).…”

Section: Methodsmentioning

confidence: 99%

Karyomorphological and heterochromatin similarities among fourVigna species

Pignone¹,

Galasso²,

Venoral

1995

Genet Resour Crop Evol

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SummaryKaryomorphological similarities among four species of the genus Vigna, V. unguiculata, V. luteola, V. oblongifolia and V. vexillata, were evaluated. Two different approaches were followed: cytomorphometrical determinations, carried out with an automated image analysis system to improve resolution, yielded some karyotypical indices; the distribution of heterochromatin bands was interpreted using a recently reported algorithm.Both methods led to similar results: the most karyologically similar species to V unguiculata appears to be V. Iuteola, while V. obIongifolia appears to be the least related one.Abbreviations: B SG -barium-saline-Giemsa banding; CMA -chromomycin A3; H 33258 -bis-benzimid derivative Hoechst 33258; DAPI -4~,6-diamidino-2-phenylindol-dihydrochloride; TF% -total form; SYi -similarity index; Rec -resemblance between chromosomes

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“…This system offers many advantages over conventional cytological and chromosomal techniques. It enables the analysis of a large number of chromosome spreads, saving the time and effort; besides, the accuracy of the data is high and the reproducibility of the image manipulation is complete and very sensitive helping to reduce error factor to minimal (KOO et al 2002;SELVI and BIGAZZI 2001;AL-TURKI et al 2000;JOSEPH et al 1999;KONIG and EBERT 1997;VENORA and SACCARDO 1993;ERRICO et al 1991;FUKUI and KAKEDA 1990).…”

Section: Discussionmentioning

confidence: 97%

Karyological studies of five taxa ofSideritisL. (Lamiaceae) sectionHesiodiaBenth. from Turkey

2008

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Mitotic karyotype analysis in theVignagenus by means of an image analyser

Abstract: SUMMARY -Detailed karyotypes of fourMarker chromosomes were also identified within each species, thus making possible the species identification by using their correspondent karyotype.

Cited by 7 publications

References 11 publications

Molecular cytogenetic characterisation and phylogenetic analysis of the seven cultivated Vigna species (Fabaceae)

Molecular cytogenetic characterisation and phylogenetic analysis of the seven cultivated Vigna species (Fabaceae)

Karyomorphological and heterochromatin similarities among fourVigna species

Karyological studies of five taxa ofSideritisL. (Lamiaceae) sectionHesiodiaBenth. from Turkey

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