Mitotic activity and acid hydrolase levels in human epidermis following a single dose of ultraviolet radiation

Mier, P.D.; Hurk, José J.M.A.; Bauer, F.W.; Grood, R.M.; Roelfzema, H.

doi:10.1111/j.1365-2133.1977.tb12538.x

Cited by 12 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the epidermis, and particularly the stratum granulosum, thickened, levels of acid phosphatase rose and reached a peak at around 3-4 days after treatment. Similar increases have been observed for other lysosomal enzymes in human epidermis following mitotic stimulus by ultraviolet radiation (Mier et al, 1977). The present results also confirm biochemically the histochemical results of Reid & Jarrett (1967), who showed that acid phosphatase in the stratum granulosum increased in activity during the hyperplastic response of normal skin and apparently normal psoriatic skin to vitamin A.…”

Section: Discussionsupporting

confidence: 91%

Changes in epidermal acid phosphatase levels in response to chemical irritation

Gibson

1982

Br J Dermatol

View full text Add to dashboard Cite

Topical sodium laurate produced little or no immediate change in the amount of rat epidermal acid phosphatase but the levels of thid enzyme progressively increased to reach a maximum at about 3 days. This coincided with acanthosis and thickening of the stratum granulosum, indicating epidermal repair. The increase depended o protein synthesis as it could be blocked by treatment with cycloheximide. This inhibitory effect was associated histologically with loss of the stratum granulosum, supporting the belief that the expression of acid phosphatase is closely linked with this layer during normal keratinization.

show abstract

Section: Discussionsupporting

confidence: 91%

Changes in epidermal acid phosphatase levels in response to chemical irritation

Gibson

1982

Br J Dermatol

View full text Add to dashboard Cite

show abstract

“…These cytokines and mediators are generally believed to exert pro-inflammatory and hyperproliferative effects on skin. At the cellular level, UV exposure causes apoptosis of some proliferating keratinocytes (sunburn cell formation) (16), and increased keratinocyte cell DNA synthesis and cell proliferation (17). Immunologically, UV exposure has been shown to cause defective Langerhans cell antigen presentation (18), induce cytokine secretion including IL-1 produced by keratinocytes, and suppress cell-mediated immunity both locally and systemically [reviewed in (19)].…”

mentioning

confidence: 99%

Effects of UVB irradiation on keratinocyte growth factor (KGF) and receptor (KGFR) expression in cultured human keratinocytes

Zhou

Lee

Kooshesh

et al. 1996

Experimental Dermatology

View full text Add to dashboard Cite

Keratinocyte growth factor (KGF) and its receptor (KGFR) are thought to play important roles in normal keratinocyte growth and differentiation. Since UVB radiation is known to influence keratinocyte growth, we sought to determine whether UVB would alter the expression of KGF and KGFR. Using a reverse-transcription coupled polymerase chain reaction (RT-PCR), the present study examined the expression of KGF and KGFR mRNA in cultured normal human keratinocytes exposed to UVB irradiation. Total cellular RNA was extracted from cultured keratinocytes at various time points after irradiation, reverse transcribed and used for PCR amplification using primers specific for KGF and KGFR. Constitutive expression of KGFR mRNA, but not KGF mRNA, was detected in normal cultured human keratinocytes. After UVB irradiation at 300 J/m2, the KGF mRNA remained undetectable while the KGFR mRNA level was significantly decreased. The down-regulation of KGFR mRNA expression was also confirmed by Northern blot analysis. Immunohistochemical studies demonstrated a decreased positive signal of KGFR in human keratinocytes after UVB irradiation. Our results suggest a possible role for the KGF-KGFR signalling pathway in the skin after exposure to UVB, and that UVB-induced growth inhibition of keratinocytes in hyperproliferative skin disorders may be related to downregulation of KGFR.

show abstract

Penetration of keratinocyte-derived cytokines into basement membrane

1997

View full text Add to dashboard Cite

Keratinocytes are known to produce a wide variety of cytokines which are believed to play a significant role in cutaneous inflammatory and immunologic reactions. Considering the array of proteolytic enzymes present in the skin and the transient nature of cytokines produced from keratinocytes, it is unclear whether cytokines released by keratinocytes cross the basement membrane and contribute to distal inflammatory and immune reactions. To investigate the ability of cytokines released from human keratinocytes to cross basement membrane, we used a two chamber culture model. Keratinocytes were plated in the upper chamber coated with a reconstituted basement membrane matrix (matrigel) on a microporous membrane. To augment cytokine production, we exposed keratinocytes to 300 J/m2 UVB; 24 h later the supernatants were collected, and the levels of cytokine were measured by ELISA. IL-1alpha, IL-6, and TNF-alpha were found to be increased after UVB irradiation in the upper chamber, and significant amounts (70-80%) of each cytokine were detected in the lower chamber. Our results indicate that keratinocyte-derived cytokines are available for interactions below the basement membrane and present circumstantial evidence that the production of those cytokines from keratinocytes contributes to the elevation of circulation after the UVB exposure.

show abstract

Mitotic activity and acid hydrolase levels in human epidermis following a single dose of ultraviolet radiation

Cited by 12 publications

References 12 publications

Changes in epidermal acid phosphatase levels in response to chemical irritation

Changes in epidermal acid phosphatase levels in response to chemical irritation

Effects of UVB irradiation on keratinocyte growth factor (KGF) and receptor (KGFR) expression in cultured human keratinocytes

Penetration of keratinocyte-derived cytokines into basement membrane

Contact Info

Product

Resources

About